Valve material with synergistic anticoagulation and anti-calcification functions and preparation method thereof
A valve and functional technology, applied in the field of valve materials with synergistic anticoagulant and anticalcification functions and its preparation, can solve problems such as easy calcification and thrombus formation, and achieve improved service life, good antithrombotic activity, and improved anticoagulant effect Effect
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Embodiment 1
[0031] A valve material with synergistic anticoagulant and anticalcification functions, the preparation method of which comprises the following steps:
[0032] (1) Cut the degreased porcine pericardium material into an appropriate size, stretch it and immerse it in a glutaraldehyde solution with a concentration of 1% and a pH value of 7, change the solution for 24 hours, and continue the treatment for 72 hours;
[0033] (2) Immerse the valve material treated in step (1) into a sealing solution (pH 7.4) containing polyethyleneimine (10mg / mL), treat it at room temperature for 0.5h, and seal the residual glutaraldehyde after cross-linking. The aldehyde group;
[0034] (3) Transfer the glutaraldehyde valve obtained in the above steps to MES buffer, add heparin (5mg / mL) and N,N'-dicyclohexylcarbodiimide (0.5mg / mL), and add Under these conditions, cross-linking treatment at 4°C for 24 hours;
[0035] (4) After washing the valve material obtained after the above reaction liquid tre...
Embodiment 2
[0037] A valve material with synergistic anticoagulant and anticalcification functions, the preparation method of which comprises the following steps:
[0038] (1) Cut the degreased pig pericardium material into a suitable size, stretch it and immerse it in a glutaraldehyde solution with a concentration of 0.5% and a pH value of 8.5 for 24 hours, and then transfer to a 1% glutaraldehyde solution Medium treatment for 48 hours;
[0039] (2) Immerse the valve material treated in step (1) into a sealing solution (pH7.4) containing polylysine (5 mg / mL), treat it at room temperature for 6 hours, and seal the glutaraldehyde cross-linked residual The aldehyde group;
[0040] (3) Transfer the glutaraldehyde valve obtained in the above steps to PBS buffer, add bivalirutin (1 mg / mL) and 1-ethyl-(3-dimethylaminopropyl) carbodiimide salt salt (0.3mg / mL), under the condition of pH6, cross-linking treatment at 25°C for 4h;
[0041] (4) After washing the valve material obtained after the a...
Embodiment 3
[0043] A valve material with synergistic anticoagulant and anticalcification functions, the preparation method of which comprises the following steps:
[0044] (1) Cut the degreased bovine pericardium material into an appropriate size, stretch it and immerse it in a glutaraldehyde solution with a concentration of 1% and a pH value of 6.5 for 24 hours, and then transfer to a 0.5% glutaraldehyde solution Medium treatment for 48 hours;
[0045] (2) Immerse the valve material after the treatment in step (1) into a sealing solution (pH 7.4) containing hexamethylenediamine (4 mg / mL), and treat it at room temperature for 3 hours to seal the residual aldehyde group after glutaraldehyde crosslinking group;
[0046] (3) Transfer the glutaraldehyde valve obtained in the above steps to PBS buffer, add hirudin (2 mg / mL) and 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride ) (0.4mg / mL), under the condition of pH5, cross-linking treatment at 37°C for 2h;
[0047] (4) After washi...
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