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Fractionation method of spirulina bioactive substances

A technology for biologically active substances and fractionation, which is applied in the preparation methods of peptides, chemical instruments and methods, and fat production. mild effect

Active Publication Date: 2020-09-22
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although there have been many studies and reports on the methods of extracting a single active substance in spirulina, such as phycocyanin, allophycocyanin, chlorophyll, polysaccharides, etc., how to extract different active substances from spirulina step by step There is still a lack of research on extracting, not breaking or less damaging other components when extracting a substance, and ensuring extraction efficiency as much as possible and avoiding the inactivation of functional substances
Which extraction methods to choose for combination, the order of extraction, how to ensure the extraction efficiency, etc., there are currently no relevant technical reports published

Method used

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  • Fractionation method of spirulina bioactive substances
  • Fractionation method of spirulina bioactive substances
  • Fractionation method of spirulina bioactive substances

Examples

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Embodiment 1

[0060] Present embodiment is a kind of Spirulina bioactive substance fractionation method, and it comprises the steps:

[0061] (1) Phycocyanin extraction and purification, including (a)-(j):

[0062] (a) Weigh 100 grams of spirulina powder, add 1150 milliliters of 0.06M potassium phosphate buffer, mix well, and make a spirulina suspension with a final concentration of about 8%, so that the suspension is as uniform as possible, and the agglomerated part can be pressed and The method of stirring makes it fully dispersed and mixed.

[0063] (b) Place the spirulina suspension in a 2-liter Erlenmeyer flask, shake and extract for 3 hours in a shaker at 40° C. at 150 rpm.

[0064] (c) Add 30 g of calcium chloride to the spirulina suspension, and continue shaking in a shaker at 40° C. for 30 minutes.

[0065] (d) Centrifuge at 4500 g for 10 minutes, and collect the supernatant.

[0066] (e) Add 600 ml of 0.06M potassium phosphate buffer solution, resuspend the precipitate, shake a...

Embodiment 2

[0082] Present embodiment is a kind of Spirulina bioactive substance fractionation method, and it comprises the steps:

[0083] (1) Phycocyanin extraction and purification, including (a)-(j):

[0084] (a) Weigh 150 grams of spirulina powder, add 1725 milliliters of 0.06M potassium phosphate buffer, mix well, and make a spirulina suspension with a final concentration of about 8%, make the suspension as uniform as possible, and the agglomerated part can be pressed and The method of stirring makes it fully dispersed and mixed.

[0085] (b) The spirulina suspension was placed in a 5-liter reactor at 40° C. and stirred for 2 hours for extraction.

[0086] (c) Add 47 grams of calcium chloride to the spirulina suspension, and continue to stir in the reactor at 40° C. for 30 minutes.

[0087] (d) Centrifuge at 4500 g for 10 minutes, and collect the supernatant.

[0088] (e) Add 600 ml of 0.06M potassium phosphate buffer solution, resuspend the precipitate, shake and extract in a sh...

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Abstract

The invention relates to a fractionation method of spirulina bioactive substances. The method sequentially comprises the following steps: S1, extracting and purifying phycocyanin in spirulina powder serving as a raw material to enable the spirulina powder to become first-grade algal residues; S2, taking the first-grade algal residues as a raw material, extracting grease in the algal residues by adopting a supercritical carbon dioxide extraction method or an organic reagent extraction method, and further performing purifying to obtain glycolipid, so that the first-grade algal residues are changed into second-grade algal residues; and S3, placing the second-grade algal residues under a ventilation condition, after an organic reagent is fully volatilized, performing sub-packaging and storage,wherein the second-grade algal residues are rich in other proteins and polysaccharides and serve as crude products of the proteins and the polysaccharides. According to the invention, different active substances can be sequentially extracted step by step in a grading manner, the method also ensures the extraction efficiency and avoids the deactivation of functional substances, and the method is high in phycocyanin and grease extraction rate, has the characteristics of simplicity in operation, low energy consumption, easiness in industrial amplification and the like, can maximize the utilization value of spirulina, and provides technical support for development of spirulina products.

Description

technical field [0001] The invention relates to the technical field of extracting natural active ingredients, in particular to a method for fractionating and separating biologically active substances from spirulina. Background technique [0002] Spirulina (Spirulina) belongs to the Cyanophyta, is a kind of filamentous prokaryote formed by the aggregation of single cells or multi-cells, also known as "Arthrospira (Arthrospira)". At present, the spirulina cultivated on a large scale at home and abroad mainly include species such as Arthrospira platensis and Arthrospira maxima. Spirulina is not only rich in nutrition, but also rich in a variety of substances with biologically active functions, mainly including phycocyanin, γ-linolenic acid, polysaccharides, etc. [0003] Phycocyanin is a natural blue pigment that is scarce in the market, and it has been widely used in the food industry in the European and American markets. Phycocyanin is also a macromolecule with a variety of...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K1/36C07K1/34C07K1/30C07K1/14C11B1/04C11B1/10C08B37/00
CPCC07K14/795C11B1/04C11B1/104C08B37/0003Y02P20/54
Inventor 袁丽韩丹翔胡强余磊杨小红
Owner INST OF AQUATIC LIFE ACAD SINICA