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A kind of spirulina bioactive substance fractionation separation method

A bioactive substance and fractionation technology, applied in the preparation method of peptides, chemical instruments and methods, fat production, etc., can solve the problems of no technical reports, disclosure, lack, etc., and achieve easy industrial scale-up, simple operation, and conditions mild effect

Active Publication Date: 2022-02-08
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although there have been many studies and reports on the methods of extracting a single active substance in spirulina, such as phycocyanin, allophycocyanin, chlorophyll, polysaccharides, etc., how to extract different active substances from spirulina step by step There is still a lack of research on extracting, not breaking or less damaging other components when extracting a substance, and ensuring extraction efficiency as much as possible and avoiding the inactivation of functional substances
Which extraction methods to choose for combination, the order of extraction, how to ensure the extraction efficiency, etc., there are currently no relevant technical reports published

Method used

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  • A kind of spirulina bioactive substance fractionation separation method
  • A kind of spirulina bioactive substance fractionation separation method
  • A kind of spirulina bioactive substance fractionation separation method

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Embodiment 1

[0060] Present embodiment is a kind of Spirulina bioactive substance fractionation method, and it comprises the steps:

[0061] (1) Phycocyanin extraction and purification, including (a)-(j):

[0062] (a) Weigh 100 grams of spirulina powder, add 1150 milliliters of 0.06M potassium phosphate buffer, mix well, and make a spirulina suspension with a final concentration of about 8%, so that the suspension is as uniform as possible, and the agglomerated part can be pressed and The method of stirring makes it fully dispersed and mixed.

[0063] (b) Place the spirulina suspension in a 2-liter Erlenmeyer flask, shake and extract for 3 hours in a shaker at 40° C. at 150 rpm.

[0064] (c) Add 30 g of calcium chloride to the spirulina suspension, and continue shaking in a shaker at 40° C. for 30 minutes.

[0065] (d) Centrifuge at 4500 g for 10 minutes, and collect the supernatant.

[0066] (e) Add 600 ml of 0.06M potassium phosphate buffer solution, resuspend the precipitate, shake a...

Embodiment 2

[0082] Present embodiment is a kind of Spirulina bioactive substance fractionation method, and it comprises the steps:

[0083] (1) Phycocyanin extraction and purification, including (a)-(j):

[0084] (a) Weigh 150 grams of spirulina powder, add 1725 milliliters of 0.06M potassium phosphate buffer, mix well, and make a spirulina suspension with a final concentration of about 8%, make the suspension as uniform as possible, and the agglomerated part can be pressed and The method of stirring makes it fully dispersed and mixed.

[0085] (b) The spirulina suspension was placed in a 5-liter reactor at 40° C. and stirred for 2 hours for extraction.

[0086] (c) Add 47 grams of calcium chloride to the spirulina suspension, and continue to stir in the reactor at 40° C. for 30 minutes.

[0087] (d) Centrifuge at 4500 g for 10 minutes, and collect the supernatant.

[0088] (e) Add 600 ml of 0.06M potassium phosphate buffer solution, resuspend the precipitate, shake and extract in a sh...

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Abstract

The invention relates to a method for fractionating and separating biologically active substances of spirulina, which carries out the following steps in sequence: S1: using spirulina powder as raw material, extracting and purifying phycocyanin in the spirulina powder, and the spirulina powder becomes first-grade Algae residue; S2: using the above-mentioned primary algae residue as raw material, using supercritical carbon dioxide extraction method or organic reagent extraction method to extract the oil in the algal residue, and further purifying to obtain glycolipids, the first-level algal residue becomes secondary algal residue Slag; S3: Place the secondary algal residue under ventilated conditions. After the organic reagent is fully volatilized, it is packaged and stored. The secondary algal residue is rich in other proteins and polysaccharides, and is used as a crude product of protein and polysaccharide. The invention can not only extract different active substances step by step, but also ensure the extraction efficiency and avoid the inactivation of functional substances. The method has a high extraction rate of phycocyanin and oil, and has the advantages of simple operation, low energy consumption, Easy industrial scale-up and other features, this scheme can maximize the value of spirulina utilization, and provide technical support for the development of spirulina products.

Description

technical field [0001] The invention relates to the technical field of extracting natural active ingredients, in particular to a method for fractionating and separating biologically active substances from spirulina. Background technique [0002] Spirulina (Spirulina) belongs to the Cyanophyta, is a kind of filamentous prokaryote formed by the aggregation of single cells or multi-cells, also known as "Arthrospira (Arthrospira)". At present, the spirulina cultivated on a large scale at home and abroad mainly include species such as Arthrospira platensis and Arthrospira maxima. Spirulina is not only rich in nutrition, but also rich in a variety of substances with biologically active functions, mainly including phycocyanin, γ-linolenic acid, polysaccharides, etc. [0003] Phycocyanin is a natural blue pigment that is scarce in the market, and it has been widely used in the food industry in the European and American markets. Phycocyanin is also a macromolecule with a variety of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/795C07K1/36C07K1/34C07K1/30C07K1/14C11B1/04C11B1/10C08B37/00
CPCC07K14/795C11B1/04C11B1/104C08B37/0003Y02P20/54
Inventor 袁丽韩丹翔胡强余磊杨小红
Owner INST OF AQUATIC LIFE ACAD SINICA