Myositis and myasthenia gravis autoantibody detection kit and method in human body fluid

A myasthenia gravis and detection kit technology, which is applied in the field of biomedicine, can solve the problems of time-consuming detection process, large demand for antibodies, and low detection sensitivity, and achieve the effects of short detection cycle, reduced pollution, and simple operation
CN111693693BActive Publication Date: 2022-03-04SHAANXI MYBIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHAANXI MYBIOTECH CO LTD
Publication Date
2022-03-04

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Abstract

The invention discloses a kit and a method for detecting autoantibodies of myositis and myasthenia gravis in human body fluid. The detection kit includes a test strip, a working solution and a closed protein solution; the detection method includes mixing the working solution and the closed protein solution to form sample dilution solution, add the sample to be tested in the sample diluent; add the sample to be tested dropwise on the sample pad of the test strip; remove the absorbent pad and sample pad on the test strip, wash the carrier film, and then wash the carrier film The absorbent pad and the gold standard pad are fixed on both ends of the carrier film; the end of the gold standard pad of the test strip is immersed in the working solution, and the color development is observed. In the detection process of the present invention, the sample chromatographic method is inverted chromatography, and the carrier membrane washing method is ultrasonic washing, which effectively increases the detection sensitivity and specificity, and the strong positive serum can still detect antibodies after being diluted more than 10,000 times.
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Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a detection kit and method for myositis and myasthenia gravis autoantibodies in human body fluid. Background technique

[0002] Idiopathic inflammatory myopathies (IIMs) are rare autoimmune diseases characterized by muscle inflammation leading to proximal muscle atrophy and disability, various skin rashes, ulcers, malignancies, etc. symptom. Autoantibodies can be detected in body fluids of more than 50% of IIM patients, so autoimmunity is considered to play an important role in the pathogenesis of myositis. Current studies have shown that these autoantibodies target nuclear and cytoplasmic proteins, and they are usually divided into two subgroups, myositis-specific autoantibodies (MSAs) and myositis-associated autoantibodies (Myositis-associated autoantibodies, MAAs). A large number of clinically relevant studies have shown that MSAs and MAAs are important mark...

Claims

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