Preparation method of spirulina polysaccharide

A technology of spirulina polysaccharides and spirulina, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problem of long extraction time of spirulina polysaccharides, the inability of spirulina to be self-sufficient, and the limitation of spirulina research, etc. problems, to achieve the effect of reducing the amount of alcohol, avoiding chemical residues, and being more practical

Inactive Publication Date: 2020-10-02
王志忠
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing methods for preparing spirulina polysaccharides, the extraction of spirulina polysaccharides takes a long time, high cost, and the activity of polysaccharides is easily damaged, so that spirulina polysaccharides cannot be truly industrially applied and produced. The technology of cultivating spirulina, as well as the large area and the place and research of breeding equipment lead to the inability of the spirulina to be self-sufficient during the experiment, which limits further research on spirulina. It reflects that there are some deficiencies in the broken wall of Spirulina cells, solid-liquid ratio, extraction temperature, extraction time, alcohol precipitation and decolorization, etc.

Method used

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  • Preparation method of spirulina polysaccharide

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Effect test

Embodiment 1

[0025] The invention provides a preparation method of spirulina polysaccharide, the specific steps of the preparation method of spirulina polysaccharide are as follows:

[0026] S1: Preparation of raw materials: 100 parts of spirulina, 10 parts of water, 100 parts of dimethyl sulfoxide, 50 parts of papain, 80 parts of trypsin, 0.2 part of powdered active polypeptide, the required auxiliary materials include: 95 parts by weight percentage 8 parts of ethanol, 10 parts of 95% phenolic formaldehyde and 5 parts of 95% acetone.

[0027] S2: Extraction of the mixture: Put the processed spirulina algae mud into the stainless steel heating pot, inject 100 parts of dimethyl sulfoxide and heat, dissolve the spirulina polysaccharide in dimethyl sulfoxide, heat to boiling, and extract 3-4h, the amount of the extract is three times that of the spirulina to obtain the presoak;

[0028] S3: Separation of the extract: inject the extracted mixture into 50 parts of papain, 80 parts of trypsin a...

Embodiment 2

[0036] The invention provides a preparation method of spirulina polysaccharide, the specific steps of the preparation method of spirulina polysaccharide are as follows:

[0037] S1: Preparation of raw materials: 200 parts of spirulina, 15 parts of water, 200 parts of dimethyl sulfoxide, 100 parts of papain, 90 parts of trypsin, 0.25 parts of powder active polypeptide, the required auxiliary materials include: 95 parts by weight percentage 9 parts of ethanol, 15 parts of 95% phenolic formaldehyde and 10 parts of 95% acetone.

[0038] S2: Extraction of the mixture: Put the processed spirulina algae mud into the stainless steel heating pot, inject 200 parts of dimethyl sulfoxide and heat, dissolve the spirulina polysaccharide in dimethyl sulfoxide, heat to boiling, and extract 3-4h, the amount of the extract is three times that of the spirulina to obtain the presoak;

[0039] S3: Separation of the extract: inject the extracted mixture into 100 parts of papain, 90 parts of trypsi...

experiment example 3

[0048] The invention provides a preparation method of spirulina polysaccharide, the specific steps of the preparation method of spirulina polysaccharide are as follows:

[0049] S1: Preparation of raw materials: 300 parts of spirulina, 20 parts of water, 300 parts of dimethyl sulfoxide, 150 parts of papain, 100 parts of trypsin and 0.3 part of powdered active polypeptide. The required auxiliary materials include: 95 parts by weight percentage 10 parts of ethanol and 20 parts of 95% phenolic aldehyde and 20 parts of 95% acetone;

[0050] S2: Extraction of the mixture: Put the processed spirulina algae mud into the stainless steel heating pot, inject 300 parts of dimethyl sulfoxide and heat, dissolve the spirulina polysaccharide in dimethyl sulfoxide, heat to boiling, and extract 3-4h, the amount of the extract is three times that of the spirulina to obtain the presoak;

[0051] S3: Separation of the extract: inject the extracted mixture into 150 parts of papain, 100 parts of t...

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Abstract

The invention discloses a preparation method of spirulina polysaccharide. The method comprises the following specific steps: preparing raw materials, extracting a mixture, separating an extract, carrying out cooling, carrying out purifying and precipitating, washing a precipitate, carrying out solid-liquid separation, and carrying out crushing and sieving. According to the invention, damage of spirulina to a polysaccharide structure in a high-temperature drying process is avoided, the yield and the quality of the polysaccharide are improved, the alcohol consumption required by decoloration isreduced, cost is saved, and the method is more practical. The extraction amount of spirulina polysaccharide is increased, the extracted polysaccharide is used for adjuvant therapy drugs on the market,and the effects of resisting tumors, resisting radiation, promoting DNA synthesis and enhancing immunity are achieved. The yield and the quality of the polysaccharide are improved, the alcohol consumption required by decoloration is reduced, the production cost can be greatly reduced, and the quality is improved. The polysaccharide is prepared by taking fresh spirulina as a raw material, any chemical reagent is not required to be added for treatment, chemical residues are avoided, and an experimental result is closer to a required result.

Description

technical field [0001] The invention relates to the technical field of spirulina polysaccharides, more specifically, the invention relates to a preparation method of spirulina polysaccharides. Background technique [0002] Spirulina, belonging to Cyanophyta, Cyanophyta, Oscillatoriaceae, and Spirulina, is an ancient low-level prokaryotic unicellular or multicellular aquatic plant. It is shaped like clockwork and spirals in blue-green, so it is also called Blue-green algae, the surface of the algae has no gelatinous sheath, it is not easy to be attached by microorganisms, there are air bubbles in the cells, and the buoyancy is good. The suitable growth temperature is generally 28°C-35°C, and 15°C and 40°C are the lowest and highest growth temperatures respectively. , and its mild and heat-resistant strains can be cultivated at 35°C-40°C. Spirulina has positive significance for the prevention and treatment of anemia. One gram of Spirulina is equal to the sum of vitamins and mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
CPCA61K31/715A61P35/00C08B37/0003C08B37/006
Inventor 王志忠穆洁王泰胡静巩东辉季祥孙君社王易王志国
Owner 王志忠
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