Application of compound amino acid in preparation of medicine for improving sensitivity of bacteria to antibiotics

A compound amino acid and antibiotic technology, applied in the field of biomedicine, can solve the problems of unsatisfactory bacterial effect and limited effect, and achieve the effects of less adverse reactions, low nitrogen content and high purity

Active Publication Date: 2020-10-09
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to overcome the limited effect of a single amino acid combined with antibiotics to improve th

Method used

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  • Application of compound amino acid in preparation of medicine for improving sensitivity of bacteria to antibiotics
  • Application of compound amino acid in preparation of medicine for improving sensitivity of bacteria to antibiotics
  • Application of compound amino acid in preparation of medicine for improving sensitivity of bacteria to antibiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 compound amino acid improves the sensitivity of cefazolin Escherichia coli drug-resistant bacteria to cefazolin

[0046] 1.1 Artificial passage of drug-resistant bacteria-cefazolin Escherichia coli-resistant bacteria

[0047]Taking Escherichia coli K12 as the starting strain, it was continuously passaged in the cefazolin liquid medium containing 1 / 2 minimum inhibitory concentration (MIC), and streaked on the plate every five generations to obtain single clones and determine their The MIC of cefazolin; until the MIC of the obtained strain is 80 μg / mL, which is 64 times that of the starting strain (for details, see figure 1 ), named as cefazolin-resistant Escherichia coli (K12-R CZO ).

[0048] 1.2 Compound amino acids improve the sensitivity of Escherichia coli and cefazolin-resistant Escherichia coli to cefazolin

[0049] Experimental materials: three compound amino acids with a total amino acid content of 5%, 8.5%, and 11.4%, respectively. The molar con...

Embodiment 2

[0076] Example 2 Compound amino acids improve the sensitivity of bacteria to antibiotics and have antibiotic specificity

[0077] 2.1 Significantly improve the bactericidal efficiency of cephalosporin antibiotics

[0078] It was further studied that after adding 0.125, 0.5, 2.0, 8.0, 32, 64 mM compound amino acids, cephalosporin antibiotics (10 μg / mL ceftriaxone, 200 μg / mL ceftiofur) acted on Escherichia coli K12 for 6 hours, and the bacterial survival rate impact; results see Figure 8 .

[0079] It can be seen from the figure that after 0.125, 0.5, 2.0, 8.0, 32, 64 mM compound amino acids synergized with ceftriaxone, the bacterial survival rates were 73.1%, 54.3%, 13.5%, 0.115%, 0.00827%, 0.00345% and 0.00173%. K12 The survival rate decreased by 1.35 times to 42352 times; after synergy with ceftiofur, the bacterial survival rate was 61.9%, 77.7%, 20.7%, 0.00907%, 0.00589%, 0.00213% and 0.000711%, and the survival rate of the sensitive bacteria K12 decreased by the fold. U...

Embodiment 3

[0089] Example 3 The effect of compound amino acids in synergy with cefazolin on the survival rate of other species of bacteria

[0090] Select other gram-negative bacteria such as Vibrio alginolyticus, Vibrio parahaemolyticus, Pseudomonas aeruginosa, and gram-positive bacteria Bacillus subtilis as the research objects to investigate the bactericidal effect of compound amino acids in synergy with cefazolin; Among them, the compound amino acid concentration was 2mM, cefazolin was 80μg / mL, and the relative survival rate of bacteria was calculated after incubation for 6h; the results are shown in Figure 12 .

[0091] It can be seen from the figure that after the addition of 2 mM compound amino acids, cefazolin reduced the survival rate of Vibrio alginolyticus from 62.1% to 13.9%, and the synergy factor was 4.5 times ( Figure 12 A); led to the reduction of the survival rate of Vibrio parahaemolyticus from 79.3% to 1.54%, and the synergy factor was 51.6 times ( Figure 12 B); l...

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Abstract

The invention belongs to the technical field of biological medicine, and particularly relates to an application of compound amino acid in preparation of a medicine for improving sensitivity of bacteria to antibiotics. The experiments prove that the compound amino acid and the antibiotics are combined for use, so that the sensitivity of bacteria to the antibiotics can be improved, the antibiotics are promoted to enter bacterial cells, the content of the antibiotics in the cells is remarkably increased, and the sterilization effect of the antibiotics is improved, thereby reducing the survival rate of the bacteria and treating diseases caused by bacterial infection.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the application of compound amino acids in the preparation of drugs for improving the sensitivity of bacteria to antibiotics. Background technique [0002] In the early twentieth century, the discovery and application of antibiotics brought bacterial infections under control. However, with the emergence of drug-resistant bacteria and superbugs, the effectiveness of antibiotics in treating bacterial diseases has been reduced or even ineffective, and human beings are facing new challenges. At present, the speed of bacteria developing drug resistance is gradually accelerating, and the difficulty and time of new drug development are gradually increasing. If this continues, there may be a dangerous situation where there will be no available drugs in the near future, seriously threatening human health and life safety. [0003] Amino acids, as small molecular compo...

Claims

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Application Information

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IPC IPC(8): A61K45/06A61K31/198A61K31/4172A61K31/405A61K31/401A61K31/546A61K31/43A61K31/407A61K31/4709A61K31/7036A61P31/04
CPCA61K45/06A61K31/198A61K31/4172A61K31/405A61K31/401A61K31/546A61K31/43A61K31/407A61K31/4709A61K31/7036A61P31/04A61K2300/00Y02A50/30
Inventor 李惠彭博彭宣宪赖诗诗
Owner SUN YAT SEN UNIV
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