Medical application of gigantol and drug for resisting propionibacterium acnes

A technology of Propionibacterium acnes and dendrobium, which is applied in the field of medicine and biology, and can solve problems such as irritation, teratogenic risk, and easy drug resistance

Active Publication Date: 2020-10-16
HEFEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, topical drugs for the treatment of acne include antibiotics and retinoic acids. The former has problems such as drug resistance, while the latter has certain irritation and potential teratogenic risks. Therefore, it is necessary to explore topical drugs that are safe and less resistant Anti-acne drugs appear particularly urgent

Method used

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  • Medical application of gigantol and drug for resisting propionibacterium acnes
  • Medical application of gigantol and drug for resisting propionibacterium acnes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Antibacterial circle experiment.

[0016] Drug preparation: dissolve dendrobium phenol with DMSO, and prepare a mother solution with a concentration of 160 mg / mL. During the experiment, the mother liquor of dendrobium was diluted 4 times with sterile liquid medium to prepare the experimental concentration of dendrobium (40 mg / mL).

[0017] Experimental method: Determination by agar diffusion method, take a 12 cm diameter plate, add 10 mL of medium. After condensation, use a sterile cotton swab to dip in different bacterial solutions (Propionibacterium acnes, Staphylococcus aureus or Escherichia coli) to be evenly spread on the surface of the culture medium, and let it dry at room temperature for 3-5 minutes. A sterile Oxford cup with a diameter of 6 mm was placed on an agar plate, and 50 μL of dendrobium at a concentration of 40 mg / mL was added to the cup. The control well was DMSO (25%), and three replicate controls were set for each condition. The diam...

Embodiment 2

[0019] Embodiment 2: Determination of minimum inhibitory concentration (MIC).

[0020] Experimental method: use the liquid dilution method to measure. In the 96-well cell culture plate, set up medium control wells, bacterial liquid control wells (Propionibacterium acnes, Staphylococcus aureus or Escherichia coli), and dendrobium liquid control wells. Equally dilute dendrobium with liquid medium (concentration: 40 mg / mL-1.25 mg / mL), add 100 μL of diluted dendrobium liquid and 10 μL of test bacteria solution (Propionibacterium acnes, golden yellow Staphylococcus or Escherichia coli), after culturing at 37°C for 24 h, the absorbance (OD) value at 600 nm wavelength of each well was detected by MTT method. The minimum inhibitory concentration (MIC) was defined as the critical drug concentration before the OD value suddenly increased.

[0021] Experimental results: as attached figure 2 As shown, the MICs of dendrobium phenol to P. acnes and Escherichia coli were both 10 mg / mL, an...

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Abstract

The invention discloses medical application of gigantol and a drug for resisting propionibacterium acnes. The gigantol is applied to preparation of the drug for resisting the propionibacterium acnes,has no toxic and side effects to human bodies, can be used as the drug for resisting the propionibacterium acnes to replace conventional antibiotic drugs which cause relapse and infections easily andis prone to produce drug resistance in some aspects and becomes the first choice clinically, and has great application prospects.

Description

technical field [0001] The invention relates to a medical application of dendrobium phenol and a medicine for resisting Propionibacterium acnes, and belongs to the field of medical biotechnology. Background technique [0002] At present, Dendrobium phenol (Gigantol, CAS: 67884-30-4), a bibenzyl phenolic substance, is extracted from the root of Dendrobium and is one of the medicinal active ingredients of Dendrobium. Dendrobium can inhibit the release of inflammatory factors in mouse macrophages induced by bacterial lipopolysaccharide through the nuclear transcription factor NF-κB pathway, and reduce the apoptosis of mouse glomerular mesangial cells induced by high glucose. In the high glucose-induced injury model of human retinal microvascular endothelial cells, it was found that dendrobium can reduce the level of intracellular reactive oxygen species and protect the injured endothelial cells. In a diabetic mouse model, it was found that the polyphenol extract of Dendrobium ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/09A61P31/04A61P17/10
CPCA61K31/09A61P31/04A61P17/10Y02A50/30
Inventor 王香存王伟张炀程彦汪义媛沈倩檀晓华秦藴怡胡洁
Owner HEFEI NORMAL UNIV
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