Microbial remediation agent and preparation method thereof

A technology of microbial remediation and bacterial agent, applied in the field of microbial remediation, can solve the problems of complex process and high treatment cost, and achieve the effect of simple treatment process, short cycle and low cost

Active Publication Date: 2020-10-16
INNER MONGOLIA HENGSHENG ENVIRONMENTAL PROTECTION TECH ENG CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The first object of the present invention is to provide a low-cost, environmentally friendly, simple treatment process that can remove toxic and harmful substances from drilling cuttings, aiming at the problems of traditional physical and chemical treatment technology for drilling cuttings, such as complicated process, high processing cost and serious secondary pollution. Long-chain hydrocarbons or organic high-molecular oils are degraded into environmentally acceptable low-molecular, water and carbon dioxide and other harmless microbial remediation bacteria agents
[0005] The second object of the present invention is to provide a low-cost, environmentally friendly, simple treatment process that can remove toxic and harmful substances from drilling cuttings, aiming at the problems of traditional physical and chemical treatment technology for drilling cuttings, such as complicated process, high processing cost, and serious secondary pollution. Preparation method of microbial remediation bacterial agent that degrades long-chain hydrocarbons or organic high-molecular oils into environmentally acceptable low-molecular, water and carbon dioxide and other harmless substances

Method used

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  • Microbial remediation agent and preparation method thereof

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Effect test

Embodiment 1

[0026] Embodiment 1: a kind of microbiological restoration bacterium agent, it comprises the raw material of following weight portion: 10 parts of Acinetobacter calcium acetate; 20 parts of Bacillus subtilis; 5 parts of Bacillus licheniformis; 15 parts of Pseudomonas aeruginosa; Dentella 25 parts; Pseudomonas saccharolyticus 15 parts; Microbacterium oxidans 5 parts; Denitrifying Achromobacter 20 parts. Among them, Acinetobacter calcium acetate has the function of degrading alkanes; Bacillus subtilis has the function of producing surfactant; Bacillus licheniformis has the function of degrading alkanes; Pseudomonas aeruginosa has the function of producing surfactant; It has the function of degrading organic oil; Pseudomonas saccharolyticum has the function of degrading organic oil; Microbacterium oxidizer has the function of degrading organic oil; Achromobacter denitrifying has the function of degrading polymer; the above bacteria can be coordinated after being combined in propor...

Embodiment 2

[0028] Embodiment 2: the preparation method of embodiment 1 microbial repair bacterial agent, it comprises the steps: (1) take bacterial classification by following weight ratio; (2) bacterial classification activation; (3) primary seed fermentation; (4) Secondary seed fermentation; (5) industrial fermentation; (6) component deployment to prepare the finished microbial restoration liquid bacterial agent; among them,

[0029] (1) Take raw materials by weight ratio as follows: 10 parts of Acinetobacter calcoacetate; 20 parts of Bacillus subtilis; 5 parts of Bacillus licheniformis; 15 parts of Pseudomonas aeruginosa; 15 parts of pseudopallidum; 5 parts of microbacterium oxidizer; 20 parts of denitrifying achromobacter;

[0030] (2) Strain activation: the bacterial strains Acinetobacter calcium acetate bacterial classification, Bacillus subtilis bacterial classification, Bacillus licheniformis bacterial classification, Pseudomonas aeruginosa bacterial classification, alkane-eating...

Embodiment 3

[0036] Embodiment 3: a kind of microbiological restoration bacterium agent, it comprises the raw material of following weight portion: 1 part of Acinetobacter calcium acetate; 10 parts of Bacillus subtilis; 1 part of Bacillus licheniformis; 5 parts of Pseudomonas aeruginosa; Dentella 1 part; Pseudomonas saccharolyticus 10 parts; Microbacterium oxidans 1 part; Denitrifying Achromobacter 1 part. Among them, Acinetobacter calcium acetate has the function of degrading alkanes; Bacillus subtilis has the function of producing surfactant; Bacillus licheniformis has the function of degrading alkanes; Pseudomonas aeruginosa has the function of producing surfactant; It has the function of degrading organic oil; Pseudomonas saccharolyticum has the function of degrading organic oil; Microbacterium oxidizer has the function of degrading organic oil; Achromobacter denitrifying has the function of degrading polymer; the above bacteria can be coordinated after being combined in proportion Coo...

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Abstract

The invention discloses a microbial remediation microbial agent and a preparation method thereof. The microbial remediation microbial agent comprises the following raw material: acinetobacter calcoaceticus; bacillus subtilis; bacillus licheniformis; pseudomonas aeruginosa; gordonia paraffinivorans; pseudochrobactrum saccharolyticum; microbacterium oxydans; and achromobacter denitrificans. The preparation method comprises the following steps: (1) weighing the strains according to the weight ratio shown in the specification; (2) strain activation; (3) primary seed fermentation; (4) secondary seed fermentation; (5) industrial fermentation; and (6) blending of the components to obtain a finished product of the microbial remediation liquid inoculant. The microbial remediation agent can degradetoxic and harmful long-chain hydrocarbon substances or organic macromolecular petroleum substances in the drilling cuttings into harmless substances such as low molecules, water and carbon dioxide which are acceptable to the environment, and the degradation rate reaches 90% or above.

Description

Technical field: [0001] The invention belongs to the technical field of microbial repair, and in particular relates to a microbial repair bacterial agent and a preparation method thereof. Background technique: [0002] Drilling cuttings produced in the process of oil and gas exploration and development are the wastes discharged from the solid control system with the drilling fluid during the drilling process. It is a multiphase stable colloidal suspension system containing clay, various chemical treatment agents, sewage , dirty oil and drill cuttings are the main pollutants produced in the process of oil and gas exploration and development. They have the characteristics of high pH, ​​high COD, high stability, and poor biodegradability. Direct discharge will cause great harm to the ecological environment. Improper disposal and excessive discharge of drilling cuttings can cause serious pollution of various environmental media such as soil, surface water and groundwater. In or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02A62D101/20C12R1/01C12R1/125C12R1/10C12R1/385
CPCC12N1/20A62D3/02A62D2101/20
Inventor 李广平姚留栓刘世军梅洋李军王瑾刘开拓王兆礼思志银王哲
Owner INNER MONGOLIA HENGSHENG ENVIRONMENTAL PROTECTION TECH ENG CO LTD
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