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Method for detecting concentration of antiepileptic drug in serum

An anti-epileptic drug and concentration technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of long sample collection time, high configuration requirements, and long sample collection time.

Pending Publication Date: 2020-10-23
南京品生医学检验实验室有限公司
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Problems solved by technology

The article "Simple and AccurateQuantitative Analysis of Ten Antiepileptic Drugs in Human Plasma by Liquid Chromatography / Tandem Mass Spectrometry" reported a method for the simultaneous detection of ten antiepileptic drugs. Only two isotope internal standards were used for the ten substances, and matrix interference would be relatively large. Sample collection The time is relatively long, and each injection takes more than 10 minutes; the article "Development, validation and clinical application of an online-SPE-LC-HRMS / MS for simultaneous quantification of phenobarbital, phenytoin, carbamazepine, and its active metabolite carbamazepine 10,11-epoxide "Introduced online solid phase extraction processing samples, high configuration requirements, long sample collection time, etc.

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  • Method for detecting concentration of antiepileptic drug in serum
  • Method for detecting concentration of antiepileptic drug in serum
  • Method for detecting concentration of antiepileptic drug in serum

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Experimental program
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Embodiment 1

[0082] 1. Experimental materials and instruments

[0083] 1. Materials

[0084] The samples came from serum samples collected from the outpatient clinic of Shanghai Zhongshan Hospital in November 2019.

[0085] (1) Instruments: Xevo TQ-S triple quadrupole mass spectrometer (Waters Corporation); UPLC I-Class ultra-high performance liquid chromatography system (with automatic sampler, Waters Corporation); SCILOGEX D2012 high-speed desktop centrifuge (USA) ; ultrapure water instrument (ELGA LabWater, UK); multi-tube vortex mixer (Vortex genie2, US); adjustable pipette (Eppendorf 0.5-10 μL, 10-100 μL, 100-1000 μL); glassware, graduated cylinder, etc. .

[0086] (2) Reagent consumables: MS grade methanol (Fisher, USA); HPLC grade methanol (Honeywell, USA); MS grade acetonitrile (Fisher, USA); HPLC grade acetonitrile (Honeywell, USA); MS grade formic acid (Fisher, USA) ;Column model: ACQUITY UPLC BEH C 18 (2.1 x 50 mm, 1.7 μm) (Waters Corporation).

[0087] (3) Standards: Stand...

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Abstract

The invention relates to a method for detecting the concentration of an antiepileptic drug in serum. The method is high in sensitivity, high in specificity, good in accuracy and simple in pretreatmentprocess, separation and detection of the antiepileptic drug in serum are completed within 6.0 min, the accuracy and precision of the method basically meet requirements, the method can be used for quantitative analysis of the antiepileptic drugs in the serum clinically, and a simple and rapid detection method is provided for concentration monitoring of the antiepileptic drugs clinically.

Description

technical field [0001] The invention belongs to the technical field of blood detection, and specifically relates to a method for detecting the concentration of antiepileptic drugs in serum, wherein the antiepileptic drugs are respectively: levetiracetam (Levetiracetam, LTT), 10-hydroxycarbazepine (Hydroxycarbazepine, HCBZ ), carbamazepine (CBZ), oxcarbazepine (Oxcarbazepine, OCP), phenytoin (5,5-Diphenylhydantoin, PHT), lamotrigine (Lamotrigine, LMT), valproic acid (2-Propylpentanoic acid, VPA), phenobarbital (Phenobarbital, PBB), topiramate (Topiramate, TPM) and clonazepam (Clonazepam, CNP). Background technique [0002] Epilepsy is a group of chronic diseases caused by a variety of etiologies, with sudden abnormal discharge of brain neurons, characterized by recurrent, paroxysmal, and stereotyped central nervous system dysfunction, leading to transient brain dysfunction. The etiology of epilepsy is complex and diverse, including genetic factors, brain diseases, systemic o...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/7266G01N2030/045
Inventor 成晓亮李美娟
Owner 南京品生医学检验实验室有限公司