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Soil biological activity and productivity evaluation method based on soil enzyme activity measurement

A biological activity and activity determination technology, applied in the field of soil detection, can solve the problems affecting soil quality, soil enzyme activity measurement efficiency and accuracy reduction, etc.

Pending Publication Date: 2020-10-30
甘肃省地质矿产勘查开发局第三地质矿产勘查院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the measurement conditions are changed, the results obtained will be very different, and the efficiency and accuracy of soil enzyme activity determination will be reduced, which will directly affect the evaluation of soil quality.

Method used

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  • Soil biological activity and productivity evaluation method based on soil enzyme activity measurement
  • Soil biological activity and productivity evaluation method based on soil enzyme activity measurement
  • Soil biological activity and productivity evaluation method based on soil enzyme activity measurement

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Soil biological activity and productivity evaluation method based on the measurement of soil enzyme activity. Due to the wide variety of soil enzymes, there are many methods for measuring their activity, but there is no unified method. At the same time, there are many pretreatment items before the measurement of enzyme activity. The standards are not consistent enough to achieve a standardized way to effectively evaluate soil biological activity and productivity. According to the degree of influence of different soil enzymes on soil biological activity and productivity such as soil fertility and soil quality, five kinds of biogeochemical cycles that can represent the biogeochemical cycle of organic source nutrients such as carbon, nitrogen, phosphorus, and sulfur in the catalyst are determined. The five soil enzymes were urease, phosphatase, β-glucosidase, arylsulfatase and N-acetyl-β-D-glucosaminidase, and the NH in 1g soil after 24h 3 The milligrams of -N represent th...

Embodiment 2

[0034] (1) Determination of urease activity

[0035] The collected soil samples were removed from the samples (leaves, stones, etc.), crushed with a ceramic mortar, mixed well, passed through a 1mm sieve, and stored in a 4°C refrigerator for later use. Take 5g of fresh soil and place it in a 50mL Erlenmeyer flask, add 10mL 10% urea solution and 20mL citrate buffer solution with a pH value of 6.7, shake well, put a cork on it, incubate in a 37°C incubator for 24h, take it out, add 20mL (1.0 mol / L KCl+0.01mol / L HCl) solution was shaken for 30min at a speed of 160r / min, and the soil suspension after shaking was centrifuged at a speed of 4000r / min for 5min and then filtered, and 3ml of the filtrate was injected into a 50ml volumetric flask, and then 4ml sodium phenoxide solution and 3ml sodium hypochlorite solution, add and shake well, develop color and constant volume after 20min, use a 1cm cuvette within 1h, compare color on a spectrophotometer at a wavelength of 578nm, compare ...

Embodiment 3

[0058] (1) Determination of phosphatase activity

[0059] The collected soil samples were removed from the samples (leaves, stones, etc.), crushed with a ceramic mortar, mixed well, passed through a 1mm sieve, and stored in a 4°C refrigerator for later use. Take 2g of fresh soil and place it in a 200mL Erlenmeyer flask. The fresh soil sample should be stored at -20°C or 4°C. Add 0.5mL of toluene in a fume hood, stopper and shake lightly. After standing for 15min, add 20mL of 0.5% Disodium phenyl phosphate, and a soil control (use 20mL water to replace the matrix of phenyl disodium phosphate solution) and no soil control (use 2mL water to replace the soil sample), shake vigorously to mix the soil sample with the solution, and then put it in 37 Cultivate in a constant temperature incubator at ℃ for 12 hours. After the incubation, take out the sample, add 40mL of 0.3% aluminum sulfate solution, filter, take 3mL of the filtrate in a 50mL colorimetric tube, add 5mL of pH 9.4 buffer...

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Abstract

The invention discloses a soil biological activity and productivity evaluation method based on soil enzyme activity determination, which comprises the following steps: selecting a soil sample, and extracting and determining the activities of urease, phosphatase, beta-glucosidase, aryl sulfatase and N-acetyl-beta-D-glucosaminidase in the soil sample; evaluating the circulation and migration capacities of carbon, nitrogen, phosphorus and sulfur in the soil sample according to the activity of the five soil enzymes so as to evaluate the biological activity and productivity of the soil; and determining conformance of culture conditions, substrate types and concentrations thereof, soil sample storage modes and the like on activity measurement of the five soil enzymes. The five represented enzymes capable of catalyzing circulation of organic source nutrient elements such as carbon, nitrogen, phosphorus and sulfur in soil are selected to evaluate the soil quality, the invention provides the rapid, effective, accurate and stable determination method of corresponding enzyme activity, so that the comparability of experimental data can be realized according to a unified enzyme activity determination method when the soil quality is evaluated, the data processing and comparison are facilitated, and the method can be popularized and applied.

Description

technical field [0001] The invention belongs to the technical field of soil detection, and in particular relates to a soil biological activity and productivity evaluation method based on soil enzyme activity measurement. Background technique [0002] As a "life-like body", almost all biochemical reactions in soil are driven by enzymes. Soil enzyme activity can be used as an important indicator of soil biological activity and productivity such as "soil fertility", "soil quality" and "soil health". Soil enzymes promote the formation of soil humus, and the transformation of organic matter and organic residues into soil into soil humus is a very complex biochemical process, which is inseparable from the catalysis of various enzymes. [0003] Soil enzymes catalyze the biogeochemical cycle of organic source nutrients such as carbon, nitrogen, phosphorus, and sulfur. Various nutrients such as carbon, nitrogen, phosphorus, and sulfur in plant residues or animal residues enter the ...

Claims

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Application Information

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IPC IPC(8): C12Q1/34C12Q1/42C12Q1/44
CPCC12Q1/34C12Q1/42C12Q1/54C12Q1/58G01N33/24C12Q1/44G01N2333/98G01N2333/942G01N2333/924
Inventor 王玉功梁军余志峰刘婧晶拉毛吉李瑞仙殷陶刚李玉泽马亮周洁孜
Owner 甘肃省地质矿产勘查开发局第三地质矿产勘查院
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