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Preparation process and application of highland barley beta-glucan extract

A technology of glucan and extract, which is applied in the field of extraction and preparation of highland barley β-glucan, which can solve the problems that the stability cannot meet the application requirements, unfavorable solid-liquid separation, difficult equipment operation, etc., and achieve good free radical scavenging And anti-radiation effect, good safety and stability, good free radical scavenging effect

Active Publication Date: 2020-11-06
BEIJING DONGFANG MIAOSEN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the patented "Method for Extracting Highland Barley β-Glucan and Dietary Fiber by Composite Enzyme Method" undergoes 17 steps to obtain the final product. During the extraction process, it is first gelatinized at high temperature (55-90°C), and then amylase is added to remove the starch. Starch gelatinization is not conducive to solid-liquid separation, and the plate-and-frame filtration method cannot be carried out. In industrial production, centrifugal filtration also has defects such as low feed-liquid yield, difficult equipment operation, high noise, and large floor space. Existing enzymes The stability of the extracted product cannot meet the needs of application in skin care products

Method used

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  • Preparation process and application of highland barley beta-glucan extract
  • Preparation process and application of highland barley beta-glucan extract
  • Preparation process and application of highland barley beta-glucan extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Preparation of barley β-glucan extract

[0060] (1) Raw material pretreatment: dry the highland barley at 50°C, crush, pass through a 0.5mm sieve, and set aside;

[0061] (2) Enzymatic extraction: the pretreated raw materials were extracted by water, the ratio of solid to liquid m / m was 1:15, the pH was adjusted to 7.5, 0.05wt% lipase was added into the water, reacted at 50°C for 1 h, and the pH was adjusted to 5.5 , add 0.05wt% pectinase into the water, and react at 50°C for 1h;

[0062] (3) Coarse filtration: use a frame filter equipped with a filter paper plate with a pore size of 15 μm to achieve solid-liquid separation and collect the coarse filtrate;

[0063] (4) Removal of starch and decolorization: Add 0.02% by weight α-high temperature resistant amylase to the crude filtrate, react at 85°C for 10 minutes, then add activated carbon with a weight percentage of 2% of the crude filtrate, heat up to 95°C for 1 hour, use Plate and frame filtration equippe...

Embodiment 2

[0067] Example 2 Preparation of barley β-glucan extract

[0068] (1) Raw material pretreatment: dry the highland barley at 70°C, crush, pass through a 1.5 mm sieve, and set aside;

[0069] (2) Enzymatic extraction: the pretreated raw materials were extracted with water, the ratio of solid to liquid m / m was 1:30, the pH was adjusted to 7, 0.01wt% lipase was added into the solvent, reacted at 60°C for 3 h, and the pH was adjusted 5.0, add 0.10wt% pectinase in the amount of solvent, and react at 55°C for 3h;

[0070] (3) Coarse filtration: use a frame filter equipped with a filter paper plate with a pore size of 25 μm to achieve solid-liquid separation and collect the crude filtrate;

[0071] (4) Removal of starch and decolorization: Add 0.05% by weight of α-high temperature resistant amylase to the crude filtrate, react at 90°C for 5 minutes, then add activated carbon with a mass percentage of 3% of the crude filtrate, heat up to 100°C for 1 hour, use Plate and frame filtratio...

Embodiment 3

[0075] Example 3 Preparation of Highland Barley-β-glucan Extract

[0076] (1) Raw material pretreatment: dry the highland barley at 60°C, crush, pass through a 1.0 mm sieve, and set aside;

[0077] (2) Enzymatic extraction: the pretreated raw materials are extracted with water, the ratio of solid to liquid m / m is 1:10, the pH is adjusted to 8, 0.10wt% lipase is added into the solvent, reacted at 40°C for 5 hours, and the pH is adjusted to 6.0 , adding 0.01wt% pectinase into the solvent, reacting at 60°C for 5h;

[0078] (3) Coarse filtration: use a frame filter equipped with a 20 μm pore size filter paper plate to achieve solid-liquid separation and collect the crude filtrate;

[0079] (4) Removal of starch and decolorization: add 0.03% by weight α-high temperature resistant amylase to the crude filtrate, react at 80°C for 15 minutes, then add 1% activated carbon to the crude filtrate, heat up to 80°C for 0.5h, Filter with a plate frame equipped with a 0.5 μm pore size filte...

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Abstract

The invention provides a preparation method and application of a highland barley beta-glucan extract. The preparation method comprises the following steps: (1) pretreatment of highland barley: dryingthe highland barley at 50-70 DEG C, crushing the highland barley and conducting sieving with a screen having a pore diameter of 0.5-1.5 mm; (2) water extraction of the pretreated highland barley: under the condition that a solid-liquid ratio m / m is 1: 10 to 1: 80, adjusting a pH value to 7-8, adding lipase accounting for 0.01-0.10 wt% of the water amount of a solvent, conducting reacting at 40-60DEG C for 1-5 hours, adjusting the pH value to 5-6, adding pectinase accounting for 0.01-0.10 wt% of the amount of the solvent, and performing reacting at 50-60 DEG C for 1-5 hours; and (3) solid-liquid separation for preparation of a coarse filtrate. According to the preparation method, the extraction rate of highland barley beta-glucan is increased, and the stability of the extract can be improved; and experiments prove that the obtained beta-glucan extract has good effects of scavenging free radicals and preventing radiation.

Description

technical field [0001] The invention belongs to the field of extraction and preparation of highland barley β-glucan, and in particular relates to the preparation of highland barley β-glucan extract suitable for skin care products. Background technique [0002] Highland barley (Hordeum vulgare. L. var. nudum Hook. f.) Also known as naked barley and barley, it belongs to one of the barley varieties of the genus Barley in the family of Gramineae in plant taxonomy, and is mainly distributed in Tibet, Qinghai, Gansu, Aba of Sichuan and Ganzi Prefecture. Highland barley is an irreplaceable staple food for farmers and herdsmen in Tibetan areas. It has the characteristics of high protein, high fiber, high vitamins, low fat, and low sugar. β-glucan is one of the main active components in highland barley. It is the main component of endosperm and aleurone cell wall, and the content is highest in aleurone cell wall (the main component of bran). β-glucan in highland barley is far away ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A61K8/73A61Q19/00
CPCC08B37/0024C08B37/0003A61K8/73A61Q19/00
Inventor 邱显荣徐定华杨雪峰李静魏铮
Owner BEIJING DONGFANG MIAOSEN BIOTECH CO LTD
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