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Preparation method of microbial agent for preventing and treating wheat root nematodes

A microbial agent and root nematode technology, applied in botany equipment and methods, biocides, nematicides, etc., can solve the problems of ozone layer destruction, high volatility, high input cost, etc., and achieve strong nematode killing ability , rapid reproduction and broad market prospects

Inactive Publication Date: 2020-11-10
LUOHE SHENGLIANG BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, chemical control mainly includes chemical toxic substances such as: methane, mianlong, metabam, chloropicrin, etc. to fumigate the land to kill nematodes. However, such drugs are often highly volatile and toxic, and will destroy the ozone layer. It is harmful to the environment. Although the biological treatment method is better than the chemical treatment method, it needs to invest a lot of cost to cultivate the natural enemies of root-knot nematodes. Not only the investment cost is high, but the time period is also long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In the present embodiment, a kind of preparation method of the microbial inoculant of preventing and treating wheat root nematode, the steps are as follows:

[0025] 1) The preparation includes the following raw materials in parts by weight: 40 parts of Bacillus subtilis-Bacillus thuringiensis mixed fermentation broth, 50 parts of jellylike Bacillus-Paecilomyces lilacinus mixed fermentation broth, 30 parts of Paenibacillus polymyxa, and Bacillus licheniformis 30 parts of Bacillus, 20 parts of Bacillus amyloliquefaciens fermentation broth, 10 parts of Verticillium chlamydoides, 10 parts of potassium fulvic acid, 4 parts of modified biochar, 1 part of chitin, 1 part of colloidal chitin;

[0026] 2) Mix Paenibacillus polymyxa, Bacillus licheniformis, and Bacillus amyloliquefaciens fermentation broth with modified biochar, potassium fulvic acid, and chitin first, and then spray Verticillium chlamydoides in the mixed raw materials and stirred evenly, then left for 8h to obta...

Embodiment 2

[0035] In the present embodiment, a kind of preparation method of the microbial inoculant of preventing and treating wheat root nematode, the steps are as follows:

[0036] 1) The preparation includes the following raw materials in parts by weight: 45 parts of Bacillus subtilis-Bacillus thuringiensis mixed fermentation broth, 55 parts of jellylike Bacillus-Paecilomyces lilacinus mixed fermentation broth, 35 parts of Paenibacillus polymyxa, Bacillus licheniformis 32 parts of Bacillus, 22 parts of Bacillus amyloliquefaciens fermentation broth, 12 parts of Verticillium chlamydoides, 12 parts of potassium fulvic acid, 6 parts of modified biochar, 1.5 parts of chitin, 1.5 parts of colloidal chitin;

[0037] 2) Mix Paenibacillus polymyxa, Bacillus licheniformis, and Bacillus amyloliquefaciens fermentation broth with modified biochar, potassium fulvic acid, and chitin first, and then spray Verticillium chlamydoides in the mixed raw materials and stirred evenly, then left for 9h to ob...

Embodiment 3

[0046] In the present embodiment, a kind of preparation method of the microbial inoculant of preventing and treating wheat root nematode, the steps are as follows:

[0047] 1) The preparation includes the following raw materials in parts by weight: 50 parts of Bacillus subtilis-Bacillus thuringiensis mixed fermentation broth, 60 parts of jellylike Bacillus-Paecilomyces lilacinus mixed fermentation broth, 40 parts of Paenibacillus polymyxa, Bacillus licheniformis 35 parts of Bacillus, 25 parts of Bacillus amyloliquefaciens fermentation broth, 15 parts of Verticillium chlamydoides, 15 parts of potassium fulvic acid, 7 parts of modified biochar, 2 parts of chitin, 2 parts of colloidal chitin;

[0048] 2) Mix Paenibacillus polymyxa, Bacillus licheniformis, and Bacillus amyloliquefaciens fermentation broth with modified biochar, potassium fulvic acid, and chitin first, and then spray Verticillium chlamydoides in the mixed raw materials and stirred evenly, then left for 10h to obtai...

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PUM

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Abstract

The invention discloses a preparation method of a microbial agent for preventing and treating wheat root nematodes, and mainly aims to solve the problems of poor effect and environmental pollution ofan existing root nematode prevention and treatment mode. The preparation method comprises the following steps: preparing the following raw materials: bacillus subtilis and bacillus thuringiensis mixedfermentation liquor, bacillus mucilaginosus and paecilomyces lilacinus mixed fermentation liquor, paenibacillus polymyxa, bacillus licheniformis, bacillus amyloliquefaciens fermentation liquor, verticillium chlamydosporium, potassium fulvate, modified biomass charcoal, chitin and colloidal chitin; stirring paenibacillus polymyxa, bacillus licheniformis, bacillus amyloliquefaciens fermentation liquor, the modified biomass charcoal, potassium fulvic acid, chitin and verticillium chlamydosporium to obtain a mixture A; mixing bacillus subtilis and bacillus thuringiensis mixed fermentation liquor,bacillus mucilaginosus and paecilomyces lilacinus mixed fermentation liquor and colloidal chitin to obtain a mixture B; and uniformly mixing the mixture A and the mixture B to obtain the microbial agent for preventing and treating the wheat root nematode.

Description

technical field [0001] The invention relates to the technical field of preparation of microbial inoculum, in particular to a method for preparing a microbial inoculum for preventing and controlling wheat root nematodes. Background technique [0002] Root nematodes, also known as root-knot nematodes, are dioecious. The larvae are elongated and worm-like, harmful to vegetables, and have a wide range of hosts, and can survive for one year without hosts. The aboveground part of the damaged plant grows short and slow, with abnormal leaf color, few fruits, low yield, and even premature death of the plant. [0003] At present, there are two main measures to kill root-knot nematodes: one is to treat the soil with chemical agents; the other is to carry out biological treatment, that is, to find the natural enemies of nematodes, expand the cultivation, and put them into the soil to carry out a virtuous biological cycle. Among them, chemical control mainly includes chemical toxic sub...

Claims

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Application Information

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IPC IPC(8): A01N63/36A01N63/23A01N63/22A01N63/20A01N43/16A01P5/00A01P21/00
CPCA01N43/16
Inventor 高岩杨世杰廖伟沈新磊郑凌云李学军杨毅
Owner LUOHE SHENGLIANG BIOLOGICAL SCI & TECH
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