A method for constructing bone morphogenetic protein sustained-release system

Abstract

The present invention aims at the lack of high-quality carrier materials for bone morphogenetic proteins during storage and use, and utilizes the universality of self-polymerization of dopamine to combine positively charged polyelectrolytes, negatively charged polyelectrolytes, and bioactive ceramic powders The slow release system of bone morphogenetic protein was constructed by integrating it on the titanium substrate by layer-by-layer assembly, which realized the long-term sustained release of bone morphogenetic protein in the lesion site, and the sustained release system had high surface modulus, It has the advantages of stability, radiation resistance, and good biocompatibility, and can effectively promote the osteogenic differentiation of bone marrow mesenchymal stem cells, and is expected to effectively promote bone repair.

Description

technical field [0001] The invention relates to the preparation of a protein sustained-release system, in particular to a method for constructing a bone morphogenetic protein sustained-release system. Background technique [0002] Bone morphogenetic protein (BMP) is an important factor in promoting osteogenesis in vivo. It can induce the proliferation and differentiation of undifferentiated mesenchymal stem cells to osteoblasts across species, promote osteogenesis, and play a role in the repair of maxillofacial bone defects, It is widely used in implantology, plastic surgery and other fields. But currently, there are the following problems in the application process: [0003] 1) BMP is easy to diffuse rapidly in body fluids, and it is also easy to be decomposed by proteases. It is difficult to maintain the therapeutic concentration, and it cannot act on more target cells within an effective time. It is difficult to fully exert its inducing activity. [0004] 2) Periodic BM...

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Problems solved by technology

[0003] 1) BMP is easy to diffuse rapidly in body fluids, and it is also easy to be decomposed by proteases. It is difficult to maintain the therapeutic concentration, and it cannot act on more target cells within an effective time. It is difficult to fully exert its inducing activity

[0004] 2) Periodic administration of BMPs usually requires invasive procedures such as injections or infusions, which are painful and cumbersome methods, and large doses and frequent administration of BMPs are also impractical and expensive

[0006] 4) The half-life of BMP is relatively short, so direct and continuous administration at the desired site is necessary

[0007] 5) For larger bone defects, BMP cannot play the role of scaffold, so a suitable carrier material is needed to cooperate with it

These materials are plentiful and can be processed into various shapes, but their biggest problem is the lack of osteoconductivity compared to allograft bone grafts, they are hydrophobic, and the degradation products will lower the pH

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