A kind of quantum dot fluorescent microsphere and preparation method thereof
A technology of fluorescent microspheres and quantum dots, applied in chemical instruments and methods, luminescent materials, measuring devices, etc., can solve the problems of reducing labeling efficiency and difficulty in controlling the concentration of activators, achieve low non-specific adsorption performance, and improve labeling efficiency , the effect of improving stability
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[0037] see figure 1 As shown, an embodiment of the present invention provides a method for preparing quantum dot fluorescent microspheres, including:
[0038]S1, dissolving the quantum dots in a mixed solution formed by an oil-soluble monomer and a cross-linking agent to obtain an oil-phase mixture, wherein the cross-linking agent contains at least one carbon-carbon double bond;
[0039] S2, disperse the oil phase mixture in the emulsifier solution to form quantum dot micelles;
[0040] S3, adding quantum dot micelles and an initiator into water, and carrying out a polymerization reaction under nitrogen protection to obtain quantum dot seed microspheres;
[0041] S4, adding functional monomers containing epoxy groups, azide groups or aldehyde groups into the polymerization reaction system, the quantum dot seed microspheres and the functional monomers are subjected to a graft reaction, and after the reaction is completed, purification treatment is performed to obtain a surface...
Embodiment 1
[0073] This embodiment provides a method for preparing quantum dot fluorescent microspheres, and the specific steps are as follows:
[0074] 1) Mix styrene and ethylene glycol diacrylate in a mass ratio of 5:20 to form a mixed solution, and then dissolve ZnCdSe / ZnS quantum dots in the mixed solution to configure a quantum dot-benzene with a quantum dot mass concentration of 20% Ethylene / ethylene glycol diacrylate solution;
[0075] 2) Weigh 0.2 g of sodium dodecyl sulfonate, dissolve it in 100 mL of water to form emulsion micelles, and then add 4 mL of 20% quantum dot-styrene / ethylene glycol diacrylate solution, which is configured as an emulsifier. The mass concentration is 0.2%, and the mixture is stirred at 20 °C for 10 minutes to make the oil phase mixture evenly dispersed in the emulsion micelles to form quantum dot micelles;
[0076] 3) Add 5 mL of styrene and 50 mL of 1 mg / mL potassium peroxodisulfate aqueous solution to the above quantum dot micelles, stir evenly, pas...
Embodiment 2
[0078] 1) Dissolving ZnCdSe / ZnS quantum dots in styrene to prepare a quantum dot-styrene solution with a quantum dot mass concentration of 20%;
[0079] 2) Weigh 0.2 g of sodium dodecyl sulfonate, dissolve it in 100 mL of water to form emulsion micelles, and then add 4 mL of 20% quantum dot-styrene solution, that is, the mass concentration configured as an emulsifier is (0.2%) , and stirred for 10 min at 20 °C to make the oil phase mixture evenly dispersed in the emulsion micelles to form quantum dot micelles;
[0080] 3) Add 5 mL of styrene and 50 mL of 1 mg / mL potassium peroxodisulfate aqueous solution to the above quantum dot micelles, stir evenly, pass nitrogen for 30 min to remove oxygen in the system, then heat up to 70 °C for 8 h, add 1 mL of methacrylic acid glycidyl ester, the reaction was continued for 4 hours, and finally the product was purified by centrifugation to obtain the quantum dot fluorescent microspheres.
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