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Application of tea polyphenol or components thereof in preparation of enterococcus faecalis bacteriophage passivator

A technology of Enterococcus faecalis and passivating agent, applied in the field of chemistry, can solve problems such as affecting body health, lysis death, intestinal flora imbalance, etc., and achieve the effect of improving intestinal health

Active Publication Date: 2020-11-17
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a probiotic in the intestinal flora, Enterococcus faecalis will also be lysed and killed by Enterococcus faecalis phages, which will lead to intestinal flora imbalance and affect the health of the body

Method used

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  • Application of tea polyphenol or components thereof in preparation of enterococcus faecalis bacteriophage passivator
  • Application of tea polyphenol or components thereof in preparation of enterococcus faecalis bacteriophage passivator
  • Application of tea polyphenol or components thereof in preparation of enterococcus faecalis bacteriophage passivator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The influence of the tea polyphenols of different concentrations of embodiment 1 on enterococcus faecalis

[0025] (1) Preparation of Enterococcus faecalis:

[0026] (A) Activation of Enterococcus faecalis: Inoculate 100 μl of Enterococcus faecalis ATCC 19433 glycerol-preserved bacteria into 50 mL of TSB liquid medium (tryptone soybean broth liquid medium), and culture it with shaking at 37°C and 220 rpm for 12~ After 15 hours, obtain Enterococcus faecalis culture solution A; then, use an inoculation ring to pick a ring of Enterococcus faecalis culture solution A on solid TSA medium (tryptone soybean broth agar medium) for streak separation, and invert culture at 37°C 18-24 hours; then pick a single colony and insert it into TSB liquid medium, and culture it with shaking at 37°C and 220rpm for 5 hours.

[0027] (B) Obtaining Enterococcus faecalis in the logarithmic growth phase: inoculate 100 μl of activated Enterococcus faecalis into 50 mL of TSB liquid medium, and cu...

Embodiment 2

[0052] The influence of the EGCG of embodiment 2 different concentrations on enterococcus faecalis phage titer

[0053] (1) Preparation of Enterococcus faecalis: same as step (1) of Example 1.

[0054] (2) Preparation of EGCG solutions with different concentrations: the operation steps are the same as step (2) in Example 1, except that TP is replaced by EGCG.

[0055] (3) Effect of EGCG on Enterococcus faecalis: the operation steps are the same as step (3) in Example 1, except that TP is replaced by EGCG.

[0056] (4) The influence of different concentrations of EGCG on the phage titer of Enterococcus faecalis: the operation steps are the same as step (4) in Example 1, except that TP is replaced by EGCG.

[0057] (5) Experimental results:

[0058] (A) Effect of a final concentration of 1.0 mg / mL EGCG on Enterococcus faecalis: the results showed that EGCG had no effect on the growth of Enterococcus faecalis.

[0059] (B) The effect of different concentrations of EGCG on the ...

Embodiment 3

[0060] The influence of the GA of embodiment 3 different concentrations on enterococcus faecalis phage titer

[0061] (1) Preparation of Enterococcus faecalis: same as step (1) of Example 1.

[0062] (2) Preparation of GA solutions with different concentrations: the operation steps are the same as step (2) in Example 1, except that TP is replaced by GA.

[0063] (3) Effect of GA on Enterococcus faecalis: the operation steps are the same as step (3) in Example 1, except that TP is replaced by GA.

[0064] (4) Effect of different concentrations of GA on the titer of Enterococcus faecalis phage: the operation steps are the same as step (4) in Example 1, except that TP is replaced by GA.

[0065] (5) Experimental results:

[0066] (A) Effect of GA at a final concentration of 1.0 mg / mL on E. faecalis: The results showed that EGCG had no effect on the growth of E. faecalis.

[0067] (B) The effect of different concentrations of GA on the titer of Enterococcus faecalis phage: the ...

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Abstract

The invention discloses an application of tea polyphenol or components thereof in preparation of an enterococcus faecalis bacteriophage passivator. The inventor finds for the first time that the tea polyphenol or the components thereof have a remarkable passivation effect on enterococcus faecalis bacteriophage and have no remarkable influence on enterococcus faecalis host bacteria. GA and EGCG arecompounded together and have a synergistic passivation effect on the enterococcus faecalis bacteriophage. The the tea polyphenol or the components thereof can be widely applied to the fields of foodengineering and medicines, and have a remarkable effect of improving intestinal health.

Description

technical field [0001] The invention belongs to the field of chemistry, and particularly relates to the application of tea polyphenols or components thereof in the preparation of enterococcus faecalis phage inactivator. Background technique [0002] Enterococcus faecalis is a type of lactic acid bacteria belonging to the genus Enterococcus of the family Streptococcus. One of the dominant flora. In healthy humans and animals, Enterococcus faecalis is a type of microorganism that normally exists in the digestive tract. It has strong tolerance and colonization ability in the intestinal mucosa, and can form a certain intestinal protective barrier. Antibacterial substances such as bacteriocin produced by Enterococcus faecalis can inhibit the growth of pathogenic bacteria such as Escherichia coli and Salmonella, regulate the balance of intestinal flora, and improve the intestinal microenvironment; Enterococcus faecalis can inhibit urease-producing bacteria and spoilage bacteria i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/06A23L33/105
CPCC12N7/00A23L33/105C12N2795/00063A23V2002/00A23V2200/32A23V2250/214A23V2250/2132
Inventor 方祥董飒爽熊嘉麒杨继国
Owner SOUTH CHINA AGRI UNIV