Method for preparing anticancer active compound CHA by utilizing sea crab symbiotic aspergillus fumigatus
A technology based on anti-cancer activity and Aspergillus fumigatus, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low yield and low CHA production, and achieve simple methods, low prices, and favorable promotion Applied effect
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Embodiment 1
[0039] A method for preparing the anticancer active compound CHA by utilizing the symbiotic Aspergillus fumigatus of sea crabs, comprising the steps of:
[0040] The first step is to prepare the fermentation medium: weigh 120 g / L of sucrose, 5 g / L of ammonium acetate, 0.84 g / L of magnesium sulfate heptahydrate, 0.016 g / L of ferrous sulfate heptahydrate, and 1.2 g / L of potassium dihydrogen phosphate L. Sodium tartrate 2 g / L and sodium glutamate 2.4 g / L, weighed, mixed and dissolved to obtain fermentation broth, put 50 mL of fermentation broth in a 250 mL Erlenmeyer flask, and sterilize in a sterilizing pot at 121°C for 30 minutes , after cooling for later use;
[0041] The second step, the operation process of seed liquid fermentation: After thawing the Aspergillus fumigatus symbiotica stored at -80°C, spot the sample on the center of the solid plate medium PDA with an inoculation needle, place it in a constant temperature incubator at 28°C, and activate it for 7 days , obtain...
Embodiment 2
[0049] A method for preparing the anticancer active compound CHA by utilizing the symbiotic Aspergillus fumigatus of sea crabs, comprising the steps of:
[0050] The first step is to prepare the fermentation medium: weigh 120 g / L of sucrose, 5 g / L of ammonium acetate, 0.84 g / L of magnesium sulfate heptahydrate, 0.016 g / L of ferrous sulfate heptahydrate, and 1.2 g / L of potassium dihydrogen phosphate L, sodium tartrate 2 g / L and sodium glutamate 2.4 g / L, mixed and dissolved after weighing to obtain a fermentation broth, 3 L of fermentation broth in a 5 L reactor, sterilized at 121 ° C for 30 min in a sterilizer, Ready to use after cooling;
[0051] The second step, the operation process of seed liquid fermentation: After thawing the Aspergillus fumigatus symbiotica stored at -80°C, spot the sample on the center of the solid plate medium PDA with an inoculation needle, place it in a constant temperature incubator at 28°C, and activate it for 7 days , obtain fresh solid plate med...
Embodiment 3
[0059] A method for preparing the anticancer active compound CHA by utilizing the symbiotic Aspergillus fumigatus of sea crabs, comprising the steps of:
[0060] The first step is to prepare the fermentation medium: weigh 120 g / L of sucrose, 5 g / L of ammonium acetate, 0.84 g / L of magnesium sulfate heptahydrate, 0.016 g / L of ferrous sulfate heptahydrate, and 1.2 g / L of potassium dihydrogen phosphate L, sodium tartrate 2 g / L and sodium glutamate 2.4 g / L, mixed and dissolved after weighing to obtain a fermented liquid, 30 L fermented liquid was packed in a 50 L reactor, sterilized by steam for 30 min, cooled for backup use;
[0061] The second step, the operation process of seed liquid fermentation: After thawing the Aspergillus fumigatus symbiotica stored at -80°C, spot the sample on the center of the solid plate medium PDA with an inoculation needle, place it in a constant temperature incubator at 28°C, and activate it for 7 days , obtain fresh solid plate medium of Aspergillu...
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