Preparation method of nutrient suitable for blood purification patient and obtained nutrient

A technology of blood purification and nutrients, applied in the field of molecular biology, to solve health problems, maintain low molecular protein concentration, and improve survival

Pending Publication Date: 2020-11-20
北京西峰科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composition of the ultrafiltrate separated by the separation membrane with this pore size is similar to that lost by blood purification patients during the blood purification process. The ultrafiltrate is injected into the patient's body as a nutrient, which can not only effectively improve blood purification. The malnutrition status of patients, especially the ability to maintain low molecular protein concentration in the body, can effectively solve the health problems of current blood purification patients

Method used

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  • Preparation method of nutrient suitable for blood purification patient and obtained nutrient
  • Preparation method of nutrient suitable for blood purification patient and obtained nutrient
  • Preparation method of nutrient suitable for blood purification patient and obtained nutrient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] This embodiment provides a method for preparing nutrients suitable for blood purification patients, specifically as follows:

[0057] Take 200mL dialysate prepared by 1.38g dialysis A powder and 0.588g dialysis B powder (both dialysis A powder and dialysis B powder were purchased from Beijing Lianyi Kang Medical Instrument Co., Ltd.), add it to 800mL normal fresh human blood, and then add 50IU heparin sodium anticoagulant, stirred evenly to obtain membrane separation stock solution, and then membrane separation.

[0058] Membrane separation using figure 1 In the device shown, the membrane separator is a dialyzer, and the dialyzer adopts a dialyzer from Fresenius Company of Germany, the model is FX80, and the pore diameter of the separation membrane used is 3.3nm, and the membrane area is 1.8m 2 .

[0059] The specific process of membrane separation is as follows: the original solution of membrane separation is passed through the dialyzer at a flow rate of 100 mL / min b...

Embodiment 2

[0067] This embodiment provides a method for preparing nutrients suitable for blood purification patients, specifically as follows:

[0068] Take 600mL normal fresh human blood, add 50IU heparin sodium anticoagulant, stir evenly, and then perform membrane separation.

[0069] Membrane separation using image 3 In the device shown, the membrane separator is a dialyzer, and the dialyzer adopts a dialyzer from Fresenius Company of Germany, the model is FX80, and the pore diameter of the separation membrane used is 3.3nm, and the membrane area is 1.8m 2 .

[0070] The specific process of membrane separation is as follows: normal blood added with anticoagulant passes through the dialyzer at a flow rate of 100mL / min through the blood pump, and at the same time, 1200mL of dialysate (that is, the diluent, which consists of 8.28g of dialyzed A powder) is added to the other side of the dialyzer. Prepared with 3.528g of dialysis B powder and deionized water), using a peristaltic pump t...

Embodiment 3

[0078] This embodiment provides a method for virus inactivation of the obtained ultrafiltrate.

[0079] Take the nutrients suitable for blood purification patients prepared by the method in Example 1, and carry out virus inactivation, as follows.

[0080] The nutrients suitable for blood purification patients prepared by the method in Example 1 were further inactivated by S / D inactivation. The specific operation is: add trinitrobutyl phosphate (TNBP) and Tween 80 to the ultrafiltrate (i.e. nutrients) so that the final mass concentrations are 0.3% and 1.0% respectively, and incubate at 25±1°C for 6h, Virus inactivation can be completed. Add soybean oil equivalent to 1 / 5 of the volume of the ultrafiltrate to the inactivated ultrafiltrate, stir evenly, let stand to separate layers, repeat the operation 3 times, extract TNBP and Tween 80, and obtain inactivated ultrafiltrate liquid.

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Abstract

The invention belongs to the technical field of molecular biology, and particularly discloses a preparation method of a nutrient suitable for a blood purification patient and the obtained nutrient. The preparation method of the nutrient comprises the step of carrying out membrane separation on any one of normal human blood, plasma and serum, wherein the pore diameter of an adopted separation membrane is 2-20nm. The components of an ultrafiltrate separated by the separation membrane with the pore diameter are similar to those of substances lost by the blood purification patient in a blood purification process, and experiments prove that when the ultrafiltrate is used as the nutrient to be input into the body of the patient, the malnutrition condition of the blood purification patient can beeffectively improved, particularly the concentration of in-vivo low-molecular-weight protein can be maintained, and the health problem of the blood purification patient at present is effectively solved.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a biological product, especially a method for preparing nutrients suitable for blood purification patients and the obtained nutrients. Background technique [0002] Blood purification is a clinical treatment method to control electrolyte and pH balance by removing toxins and wastes from the body and removing excess body fluids from the body. Blood purification can replace part of the renal function and is the main alternative treatment for patients with end-stage renal disease. For patients with end-stage renal disease, regular maintenance blood purification therapy can prolong the patient's survival period, improve the quality of life, and reduce complications. [0003] However, loss of appetite, indigestion, and gastrointestinal dysfunction due to micro-inflammatory state, accumulation of toxins, and drugs for treatment of complications (such as iron and phosphorus b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/38A61K35/14A61K31/198A61K31/568A61K31/573A61K31/575A61K31/7004A61K31/714A61K38/04A61K38/08A61K38/17A61K38/22A61K38/24A61K38/27A61K38/28A61K38/35A61P3/02
CPCA61K35/14A61K31/198A61K31/7004A61K31/568A61K31/714A61K38/28A61K31/575A61K38/27A61K38/22A61K38/35A61K38/24A61K38/38A61K38/085A61K38/043A61K38/1729A61K38/1754A61K31/573A61P3/02
Inventor 刘自军李建中
Owner 北京西峰科技有限责任公司
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