A Strain of Trametes pilosae with Efficient Decolorization of Lignin

A technology of Trametes and lignin, applied in the field of microbial engineering, can solve the problems of low lignin enzyme activity, high enzyme load, and low decolorization efficiency, and achieve the effect of short decolorization time and simple operation

Active Publication Date: 2022-04-15
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently studied strains of white rot fungi have shortcomings such as generally low ligninase activity, high enzyme load, and low decolorization efficiency, and there are few strains with industrial application prospects.

Method used

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  • A Strain of Trametes pilosae with Efficient Decolorization of Lignin
  • A Strain of Trametes pilosae with Efficient Decolorization of Lignin
  • A Strain of Trametes pilosae with Efficient Decolorization of Lignin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: Isolation, screening and species identification of Trametes hirsuta X-13 strain

[0053] 1. Separation and screening

[0054] The fruiting bodies of fungi growing well on dead trees or fallen trees in Yuelu Mountain, Hunan Province were washed with sterile water and wiped with 75% alcohol, and then cut into pieces with a sterilized scalpel in an ultra-clean bench. 0.5cm×0.5cm bacterial block, the bacterial block was inoculated on the PDA agar medium, and placed in a constant temperature incubator at 28°C for cultivation.

[0055] After the fungal mycelium grows on the PDA agar medium, cut off the 0.5cm×0.5cm mycelium with a thin layer of medium with an inoculation spatula, and inoculate it in a new PDA agar medium.

[0056] Obtain pure colonies of microorganisms after repeated inoculation and culture for 2 to 3 generations, inoculate the isolated pure colonies on PDA-aniline blue agar medium and PDA-guaiacol agar medium respectively, and place them in a c...

Embodiment 2

[0062] Example 2: Detection of lignin-degrading enzyme produced by Trametes hirsuta X-13 strain

[0063] The Trametes hirsuta X-13 mycelium obtained in Example 1 was inoculated on the PDA-guaiacol agar medium, and then the PDA-guaiacol agar medium was inverted at 28°C for constant temperature cultivation In the box, cultivate for 3-5 days, observe and record the discoloration of the culture medium around the colony.

[0064] The result is as Figure 5-6 As shown, compared with the control that was not inoculated with Trametes hirsuta X-13, the colonies on the PDA-guaiacol agar medium inoculated with this bacterium had larger and clear reddish-brown around and at the bottom circle, the diameter of the reddish-brown circle is 31 mm, which indicates that the strain has a strong lignin-degrading enzyme ability.

Embodiment 3

[0065] Embodiment 3: the detection of peroxidase produced by Trametes hirsuta (Trametes hirsuta) X-13 strain

[0066] The Trametes hirsuta X-13 mycelium obtained in Example 1 was inoculated on the PDA-aniline blue agar medium, and then the PDA-aniline blue agar medium was placed upside down in a 28°C constant temperature incubator, and cultivated From 3 to 5 days, observe and record the decolorization of the culture medium around the colony.

[0067] The result is as Figure 7-8 As shown, compared with the control, the blue color of the PDA-aniline blue agar medium inoculated with the bacterium almost disappeared, the diameter of the faded circle was 55mm, and the hyphae still showed natural color, which indicated that the bacterium could degrade and utilize Aniline blue, instead of adsorbing aniline blue, which indicates that the strain has a strong ability to produce lignin peroxidase or manganese peroxidase.

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Abstract

The invention discloses a strain of Trametes hirsuta X‑13 with high-efficiency decolorization of lignin, which belongs to the technical field of microbial engineering. After culturing for 11 days under the condition of ℃ and 180rpm, the decolorization rate of the bacteria to lignin can reach 52.43%. The decolorization effect of this strain on lignin-containing solution is better than that of most strains of the same species reported at home and abroad, and the decolorization time is shorter and the operation is simple. It is suitable for industrial treatment of lignin-containing waste liquid such as paper industry waste liquid .

Description

technical field [0001] The invention relates to a high-efficiency decolorization of lignin Trametes pilosa, which belongs to the technical field of microbial engineering. Background technique [0002] With the continuous increase of people's demand for different kinds of paper products, the paper industry has developed rapidly, but a large amount of colored wastewater produced in the production process of the paper industry is becoming an increasingly serious source of pollution. The waste liquid of the paper industry contains a large amount of lignin and its How to effectively treat paper industry waste liquid and reduce its impact on the environment is an important problem to be solved urgently. [0003] Lignin is an insoluble polymer with a three-dimensional network space structure formed by styrene-acrylic units of different structures connected to each other through carbon-carbon bonds and ether bonds. Natural lignin is insoluble in water and most solvents, and it is di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N9/02C12N9/08C02F3/34C02F103/28C12R1/645
CPCC12N1/14C12N9/0061C12N9/0065C12Y110/03002C12Y111/01013C02F3/347C02F2103/28C12R2001/645C12N1/145
Inventor 刘高强马江山张家顺刘勇男王晓玲
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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