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Polyethyleneimine modified porous silicon dioxide-coating LDH core-shell nano composite material and preparation method thereof

A technology of porous silica and nanocomposite materials, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve problems such as poor biological stability, and achieve the effect of low cost, simple operation, and promotion of DC maturity.

Inactive Publication Date: 2020-12-01
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention is to solve the existing LDH@SiO 2 The plasmid DNA combined with the shell-core nanocomposite material is easily degraded by nucleases and has poor biological stability. A polyethylenimine modified porous silica@LDH core-shell nanocomposite material and its preparation method are provided.

Method used

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  • Polyethyleneimine modified porous silicon dioxide-coating LDH core-shell nano composite material and preparation method thereof
  • Polyethyleneimine modified porous silicon dioxide-coating LDH core-shell nano composite material and preparation method thereof
  • Polyethyleneimine modified porous silicon dioxide-coating LDH core-shell nano composite material and preparation method thereof

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specific Embodiment approach 1

[0039] Specific Embodiment 1: In this embodiment, polyethyleneimine modified porous silica@LDH core-shell nanocomposite is a nanomaterial with shell-core structure, in which the shell is LDH, and the core is polyethyleneimine modified silica .

specific Embodiment approach 2

[0040] Specific embodiment 2: The preparation method of polyethyleneimine modified porous silica@LDH core-shell nanocomposite in this embodiment includes the following steps:

[0041] 1. Preparation of aminated porous silica:

[0042] Weigh cetyltrimethylammonium bromide at room temperature and dissolve in deionized water, add ether, ammonia water and ethanol under magnetic stirring, stir at 1000-5000r / min for 30-120min; mix ethyl orthosilicate with KH550 rapidly Add it dropwise into the reaction system, stir at 1000-5000r / min for 4-8h, add HCl to the system after the reaction is over to terminate the reaction; centrifuge the obtained reaction solution at 8000-12000r / min for 10-15min, discard the supernatant, and use it for precipitation After washing with absolute ethanol for 3-5 times, disperse in ethanol, then add HCl, stir at 70-75°C for 24-26 hours, then centrifuge at 8000-12000r / min for 15-20min, wash the precipitate with absolute ethanol for 3-5 and dry at room tempera...

specific Embodiment approach 3

[0050] Specific embodiment 3: The difference between this embodiment and specific embodiment 2 is that the volume ratio of the mass of cetyltrimethylammonium bromide to deionized water in step 1 is 0.5 g: (70-75) mL. Others are the same as in the second embodiment.

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Abstract

The invention discloses a polyethyleneimine modified porous silicon dioxide-coating LDH core-shell nano composite material and a preparation method thereof, and relates to a core-shell structure nanocomposite material and a preparation method thereof. The problems that plasmid DNA combined by an existing LDH-coated SiO2 shell-core nano composite material is easily degraded by nuclease, and the biological stability is poor are solved. The method comprises the following steps: 1, preparing aminated porous silicon dioxide; 2, preparing carboxylated porous silicon dioxide; 3, preparing polyethyleneimine modified porous silicon dioxide; and 4, preparing the polyethyleneimine modified porous silicon dioxide-coating LDH core-shell nano composite material. According to the core-shell nano composite material prepared by the method, plasmid DNA cannot be recognized by protease and nuclease and cannot be degraded, and the core-shell nano composite material has very high biological stability. Theinvention is applied to the field of gene delivery vector materials.

Description

technical field [0001] The invention relates to a core-shell structure nanocomposite material and a preparation method thereof. Background technique [0002] Silica nanoparticles have attracted increasing attention due to their high thermal / chemical stability, large specific surface area, uniform and controllable pore size and particle size, and strong modifiability. At the same time, silica nanoparticles can be biodegraded in the body to generate silicic acid, which can be absorbed by the human body or excreted through the urinary system, which has good biological safety. These characteristics of silica nanoparticles make it a potential nano-drug carrier and gene carrier, with many advantages, such as protecting DNA from nuclease degradation, good penetration effect, and can directly enter cells through biomembrane barriers, Plasmid DNA is released and the corresponding protein is expressed; it is effectively taken up by antigen-presenting cells, processed and presented to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/02A61K47/04A61K47/32B82Y5/00
CPCA61K9/5115A61K9/5138B82Y5/00
Inventor 赵凯金政
Owner HEILONGJIANG UNIV
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