Preparation method of chlorophyll copper-modified dendrimer copper complex nano diagnosis and treatment material
A technology of dendrimer and copper chlorophyll, applied in the field of preparation of functional nanomaterials, can solve problems such as undiscovered combination therapy
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Embodiment 1
[0072] (1) Dissolve copper chlorophyllin (13.88mg) in 10mL of DMSO, add EDC (18.4mg) and NHS (11.06mg) and stir for 1h, then add the solution dropwise to 50mL of the fifth generation polyamide-amine type Dendrimer G5.NH 2 (50mg) in an ultrapure aqueous solution (1mg / mL) and stirred for 72h. Then use a dialysis bag with a molecular weight cut-off of 5000 to dialyze for 3 days (2L / time, 3 times / day), and freeze-dry to obtain the dendrimer G5.NH modified by copper chlorophyll 2 -Cu Chl.
[0073] (2) Dissolve 56.9 mg of G5.NHAc-Cu Chl in ultrapure water (5 mL), add triethylamine (24.72 mg) and mix well, add acetic anhydride (24.9 mg) dropwise, stir for 24 hours, and then use MWCO Dialyze a dialysis bag of 5000 for 3 days (2 L / time, 3 times / day), and freeze-dry to obtain the acetylated dendrimer-copper chlorophyll conjugate G5.NHAc-Cu Chl.
[0074] (3) Dissolve G5.NHAc-Cu Chl (33.82mg) in physiological saline, add copper chloride dihydrate solid (8.73mg) and ultrasonically dispe...
Embodiment 2
[0079] Accurately weigh the G5.NH prepared in Example 1 2 -Cu Chl (0.86mg) was dissolved in 1mL aqua regia and the reaction was complete. After adding 3 mL of ultrapure water for dilution, ICP was used to test, and the concentration of copper element in the solution was 5.33 μg / ml. From this calculation, it was calculated that 5.1 copper chlorophyllins were connected to each acetylated fifth-generation dendrimer on average.
[0080] Weigh 1.2mg of G5.NH prepared in Example 1 2 -Cu Chl was tested using a thermogravimetric analyzer with a temperature range from 50°C to 800°C and a heating rate of 20°C / min. The results are attached figure 2 , the residual mass percentage measured at 800°C is 14.5%, which is relatively close to the ICP test result.
Embodiment 3
[0082] The G5.NHAc-Cu Chl, G5.NHAc-Chl / Cu, G5.NHAc and copper chlorophyll prepared in Example 1 were dissolved in ultrapure water to test the ultraviolet-visible spectrum. as attached image 3 As shown in A, the characteristic absorption peaks of copper chlorophyll are at 407nm and 630nm. as attached image 3 As shown in B, the maximum absorption peak of copper chloride aqueous solution is at 812nm; the acetylated dendrimer G5.NHAc has no obvious absorption peak at 400-1000nm; the acetylated dendrimer G5.NHAc-modified by chlorophyll copper Cu Chl and copper chlorophyll modified acetylated dendrimer copper complex G5.NHAc-Chl / Cu has characteristic absorption peaks at 400-1000nm consistent with copper chlorophyll. Among them, the acetylated dendrimer copper complex G5.NHAc-Chl / Cu modified by copper chlorophyll has no characteristic absorption peak at 812nm, indicating that there is no free copper ion in the solution.
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