Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bone hemostatic material with osteogenesis inducing activity and preparation method thereof

A technology of hemostatic material and osteogenic activity, which is applied in the field of bone hemostatic material with inductive osteogenic activity and its preparation, and can solve the problems of bone implant material requirements, failure to achieve hemostatic effect, and unsatisfactory biocompatibility, etc. Achieve good biocompatibility, short production cycle and low production cost

Inactive Publication Date: 2020-12-04
TIANJIN ZHONGJIN BIOLOGICAL DEV CO LTD
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these materials are degradable, they are far less adhesive than traditional bone wax, and are easily washed away by the blood flow during the operation, failing to achieve the expected hemostatic effect.
Moreover, the biocompatibility of most of the above materials cannot meet the requirements of bone implant materials, and there are biological safety problems in cytotoxicity, blood compatibility, pyrogen reaction, etc.
Therefore, there is no ideal orthopedic hemostatic material at present.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bone hemostatic material with osteogenesis inducing activity and preparation method thereof
  • Bone hemostatic material with osteogenesis inducing activity and preparation method thereof
  • Bone hemostatic material with osteogenesis inducing activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A method for preparing a bone hemostatic material with osteogenic activity, comprising the steps of:

[0054] (1) Grinding: use a grinder to grind the cortical bone, and use liquid nitrogen to keep the temperature low during the grinding process. The cortical bone blocks were crushed into 100-6 mesh cortical bone particles.

[0055] (2) Degreasing: The degreasing solution is a mixture of chloroform / methanol with a volume ratio of 1:1. Add 0.1 times of methanol and 0.2 times of chloroform to the reaction tank in turn, and react at room temperature After 12 hours, replace the degreasing solution and degrease again.

[0056] (3) Decalcification: Prepare a decalcification solution (0.6mol / L hydrochloric acid), soak the cortical bone particles in the decalcification solution, and the decalcification solution just submerges the cortical bone particles. The reaction was carried out at room temperature for 26 hours. Replace the decalcification solution and decalcify twice ag...

Embodiment 2

[0063] A method for preparing a bone hemostatic material with osteogenic activity, comprising the steps of:

[0064] (1) Grinding: use a grinder to grind the cortical bone, and use liquid nitrogen to keep the temperature low during the grinding process. The cortical bone blocks were crushed into 100-6 mesh cortical bone particles.

[0065] (2) Degreasing: The degreasing solution is a mixture of chloroform / methanol with a volume ratio of 1:1. Add 0.3 times of methanol and 0.7 times of chloroform to the reaction tank in turn, and react at room temperature After 16 hours, replace the degreasing solution and degrease again.

[0066] (3) Decalcification: prepare a decalcification solution (0.5mol / L hydrochloric acid), soak the cortical bone particles in the decalcification solution, and the decalcification solution just covers the cortical bone particles. The reaction was carried out at room temperature for 28 hours. Replace the decalcification solution and decalcify twice again...

Embodiment 3

[0073] A method for preparing a bone hemostatic material with osteogenic activity, comprising the steps of:

[0074] (1) Grinding: use a grinder to grind the cortical bone, and use liquid nitrogen to keep the temperature low during the grinding process. The cortical bone blocks were crushed into 100-6 mesh cortical bone particles.

[0075] (2) Degreasing: The degreasing solution is a mixture of chloroform / methanol with a volume ratio of 1:1. Add methanol 0.5 times the weight of cortical bone grains and chloroform 1.5 times the weight of the cortical bone into the reaction tank in turn, and react at room temperature After 18 hours, replace the degreasing solution and degrease again.

[0076] (3) Decalcification: Prepare a decalcification solution (0.4mol / L hydrochloric acid), soak the cortical bone particles in the decalcification solution, and the decalcification solution just covers the cortical bone particles. The reaction was carried out at room temperature for 28 hours. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Molecular weightaaaaaaaaaa
Molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention provides a preparation method of a bone hemostatic material with osteogenesis inducing activity and the bone hemostatic material prepared by the preparation method. The bone hemostatic material comprises an active bone extract accounting for 0.5-5% of the mass of the bone hemostatic material, and further comprises a dispersing agent accounting for 10-23% of the mass of the bone hemostatic material and a powder adjuvant accounting for 72.9-89.5% of the mass of the bone hemostatic material, wherein the active bone extract comprises the following components in percentage by mass: 21.3-62% of a coagulant, 19.5-53.7% of a binder, 2.7-11% of a regulator, 6.1-23.4% of an osteogenesis inducing agent and 1.4-7.5% of a bacteriostatic agent. The invention further provides the preparation method of the hemostatic material. According to the bone hemostatic material with osteogenesis inducing activity and the preparation method thereof, the bone hemostatic material has good adhesion and good hemostatic effect, can be degraded, avoids the problem that a bone wound occupies a space or is difficult to repair due to the existence of foreign matters, and has good biological safety and biocompatibility.

Description

technical field [0001] The invention belongs to the technical field of bone hemostatic materials, and in particular relates to a bone hemostatic material with osteogenic activity and a preparation method thereof. Background technique [0002] Clinically, bone wound bleeding is difficult to stop bleeding by vasoconstriction, and conventional intraoperative hemostatic methods such as electrocoagulation, clamps, and hemostatic materials are not effective. The commonly used bone hemostatic material in clinic is bone wax. The hemostatic bone wax generally contains beeswax or vaseline. The principle of hemostasis is to mechanically plug the capillary bleeding of the bone wound surface. However, this kind of material is not degradable. The presence of foreign bodies causes bone wounds to occupy space, and makes the bone wounds difficult to repair and cannot heal well. In addition, it also increases the risk of postoperative infection. [0003] Researchers at home and abroad have d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L24/00
CPCA61L24/0005A61L24/001A61L24/0015A61L24/0042A61L2300/412A61L2400/04A61L2430/02
Inventor 李湘杰任芳丁力王长江盖增祝君梅朱晓丽刘红云聂卫
Owner TIANJIN ZHONGJIN BIOLOGICAL DEV CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com