Unlock instant, AI-driven research and patent intelligence for your innovation.

Preservation method of rhBMP-2-containing autologous skull flap with periosteum

A preservation method, the technology of rhbmp-2, is applied in the fields of medicine and bioengineering, which can solve the problems of uneven quality of bone flaps, high incidence of infection and absorption, increased patient pain and unnecessary disputes, and achieve simple process, Improve the probability of successful healing growth and the effect of easy operation

Active Publication Date: 2020-12-08
江西省元化低温医学科技有限公司
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, the quality of the preserved bone flap varies, and the incidence of infection and absorption after replantation is high, which leads to the patient's second operation, increases the patient's pain and unnecessary disputes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. The skull flap with periosteum removed during the operation (including the gnawed skull fragments) is placed in a preservation box with preservation solution C, and transported by cold chain to a 100,000-level ultra-clean aseptic operating table.

[0039] Preservation solution C is glycerol-fructose solution containing 10% glycerin and 500 U / ml gentamicin.

[0040] 2. Clean blood stains with isotonic saline on an ultra-clean aseptic operating table, and use a scalpel to remove soft tissues other than the periosteum. After cleaning up, soak in 2% povidone iodine solution for 30 minutes, and then use 75% alcohol to clean and remove iodine. Finally, rinse with isotonic saline and dry with sterile gauze.

[0041] 3. On an ultra-clean aseptic operating table, use a drill to grind a hole on the inner plate of the skull with a diameter of 5mm, and stop when it reaches the outer plate of the skull without piercing the outer plate. At an interval of about 5cm, drilled skull...

Embodiment 2

[0050] 1. The skull flap with periosteum removed during the operation (including the gnawed skull fragments) is placed in a preservation box with preservation solution C, and transported by cold chain to a 100,000-level ultra-clean aseptic operating table.

[0051] Preservation solution C is a glycerol-fructose sodium chloride solution containing 30% glycerol, and contains 0.8mg / ml levofloxacin.

[0052] 2. Clean blood stains with isotonic saline on an ultra-clean aseptic operating table, and use a scalpel to remove soft tissues other than the periosteum. After cleaning up, soak in 2% povidone iodine solution for 30 minutes, and then use 75% alcohol to clean and remove iodine. Finally, rinse with isotonic saline and dry with sterile gauze.

[0053] 3. On an ultra-clean aseptic operating table, use a drill to grind a hole on the inner plate of the skull with a diameter of 4mm, and stop when it reaches the outer plate of the skull without piercing the outer plate. At an interv...

Embodiment 3

[0062] 1. The skull flap with periosteum removed during the operation (including the gnawed skull fragments) is placed in a preservation box with preservation solution C, and transported by cold chain to a 100,000-level ultra-clean aseptic operating table.

[0063] Preservation solution C is a hydroxyethyl starch solution containing 75% glycerol and 1000 U / ml gentamicin.

[0064] 2. Clean blood stains with isotonic saline on an ultra-clean aseptic operating table, and use a scalpel to remove soft tissues other than the periosteum. After cleaning up, soak in 2% povidone iodine solution for 30 minutes, and then use 75% alcohol to clean and remove iodine. Finally, rinse with isotonic saline and dry with sterile gauze.

[0065] 3. On an ultra-clean aseptic operating table, use a drill to grind a hole on the inner plate of the skull with a diameter of 6mm, and stop when it reaches the outer plate of the skull without piercing the outer plate. At an interval of about 4cm, drilled ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preservation method of a rhBMP2-containing autologous skull flap with periosteum, the process is simple, operation is convenient, the method is an autologous skull flap in-vitro low-temperature preservation method of which the activity and the sterility of the skull flap can be kept to the maximum extent, meanwhile, recombinant human bone morphogenetic protein 2 (rhBMP2) is added, the probability of successful healing and growth of the skull flap is increased. The occurrence of skull flap absorption is reduced.

Description

technical field [0001] The invention relates to the fields of medicine and bioengineering, in particular to a method for preserving rhBMP-2-containing autologous skull flap with periosteum. Background technique [0002] Decompressive craniectomy is a commonly used treatment technique in neurosurgery. Brain tumors, cerebral hemorrhage, and craniocerebral trauma cause intracranial hypertension, which cannot be controlled by drugs. Decompressive craniectomy is required. In patients with large-scale skull defects, local scalp subsidence due to atmospheric pressure may lead to imbalances in intracranial pressure, brain tissue displacement, decreased blood flow in the cerebral hemispheres, and cerebrospinal fluid circulation disorders, resulting in a series of clinical manifestations, mainly including: Headaches, dizziness, irritability, seizures, unexplained malaise, and various mental disorders. Cranioplastic surgery can not only repair skull defects, restore the appearance and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0215A01N1/0221A01N1/0226A01N1/0294
Inventor 林卫军江柏龄
Owner 江西省元化低温医学科技有限公司