Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hyaluronic acid artesunate, nano-micelle preparation of hyaluronic acid artesunate, and preparation method and use of nano-micelle preparation

A technology of artesunate and nano micelles, applied in nanotechnology, nanotechnology, nanomedicine, etc., to achieve the effects of good responsive release characteristics, strong tumor cytotoxicity, and strong targeting

Pending Publication Date: 2020-12-18
XIAMEN UNIV
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But up to now, there is no report of a kind of nano drug preparation combining ASA and HA

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hyaluronic acid artesunate, nano-micelle preparation of hyaluronic acid artesunate, and preparation method and use of nano-micelle preparation
  • Hyaluronic acid artesunate, nano-micelle preparation of hyaluronic acid artesunate, and preparation method and use of nano-micelle preparation
  • Hyaluronic acid artesunate, nano-micelle preparation of hyaluronic acid artesunate, and preparation method and use of nano-micelle preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Dissolve ASA in dimethyl sulfoxide, add the activator EDC·HCl according to the molar ratio of ASA and activator as 1:2, carry out carboxyl activation at 45°C for 0.5h under the protection of nitrogen, and then add The formamide solution of HA, the molar ratio of ASA to HA is 1:2, and the esterification reaction is carried out at 45°C for 48h under the protection of nitrogen;

[0049](2) Dialyzing the material obtained in step (1) to obtain HA-ASA dialyzate;

[0050] (3) After the HA-ASA dialysate obtained in step (2) is vacuum freeze-dried, the corresponding HA-ASA raw material is obtained;

[0051] (4) Dissolving the HA-ASA raw material obtained in step (3) in dimethyl sulfoxide to obtain an organic solution of HA-ASA, and ultrasonically dispersing at room temperature for 0.5 h;

[0052] (5) The organic solution of HA-ASA obtained in step (4) was slowly added to 9 times the volume of deionized water, placed on a shaking table at room temperature for 3 hours, and t...

Embodiment 2

[0056] (1) Dissolve ASA in dimethyl sulfoxide, add the activator EDC·HCl according to the molar ratio of ASA and activator at a ratio of 1:2, carry out carboxyl activation at 50°C for 1 h under nitrogen protection, and then add HA formamide solution, the molar ratio of ASA to HA is 1:2, and the esterification reaction is carried out at 50°C for 36h under the protection of nitrogen;

[0057] (2) Dialyzing the material obtained in step (1) to obtain HA-ASA dialyzate;

[0058] (3) After the HA-ASA dialysate obtained in step (2) is vacuum freeze-dried, the corresponding HA-ASA raw material is obtained;

[0059] (4) dissolving the HA-ASA raw material obtained in step (3) in dimethyl sulfoxide to obtain an organic solution of HA-ASA; ultrasonically disperse at room temperature for 1 h;

[0060] (5) Slowly add the organic solution of HA-ASA obtained in step (4) to 9 times the volume of deionized water, place it on a shaker at room temperature for 1 hour, and then dissolve the HA-ASA...

Embodiment 3

[0064] (1) Dissolve ASA in dimethyl sulfoxide, add the activator EDC·HCI according to the molar ratio of ASA and activator as 1:2, carry out carboxyl activation at 45°C for 2 hours under the protection of nitrogen, and then add HA formamide solution, the molar ratio of ASA to HA is 1:2, and the esterification reaction is carried out at 45°C for 36h under the protection of nitrogen;

[0065] (2) dialyze the material obtained in step (1) to obtain HA-ASA dialysate;

[0066] (3) After the HA-ASA dialysate obtained in step (2) is vacuum freeze-dried, the corresponding HA-ASA raw material is obtained;

[0067] (4) Dissolving the HA-ASA raw material obtained in step (3) in N,N-dimethylformamide to obtain an organic solution of HA-ASA; ultrasonically disperse at room temperature for 0.5h;

[0068] (5) Slowly add the organic solution of HA-ASA obtained in step (4) to 9 times the volume of deionized water, place it on a shaker at room temperature for 3 hours, and then perform dialysis...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses hyaluronic acid artesunate, a nano-micelle preparation of hyaluronic acid artesunate, and a preparation method and use of the nano-micelle preparation. The active ingredient hyaluronic acid artesunate consists of artesunate and hyaluronic acid, wherein artesunate serves as a main active ingredient, hyaluronic acid serves as a carrier and a targeting molecule and is connected with artesunate through a chemical bond to form hyaluronic acid artesunate, and then self-assembly is performed to form a nano-micelle and freeze-drying is performed to obtain the nano-micelle preparation. A particle size of the hyaluronic acid artesunate nano-micelle is 100-200 nm, the freeze-dried preparation is good in stability, and has a relatively good acidity responsive release characteristic after being injected into a body, and cytotoxicity tests prove that compared with the artesunate pure medicine, the nano-micelle preparation has stronger tumor cytotoxicity effects. The nano-micelle preparation has the characteristics of strong targeting property, long circulation and low toxicity.

Description

technical field [0001] The invention belongs to the technical field of antitumor drugs, and in particular relates to a nano-micelle preparation and its preparation method and application. Background technique [0002] Artesunate (Artesunate, abbreviated as ASA), Mr 384.42, the chemical name is dihydroartemisinin-l,2-α-succinic acid monoester, is a new antimalarial drug artemisinin with sesquiterpene lactones One of the important derivatives. As a new antimalarial drug with a new structure, ASA has the characteristics of high efficiency, quick action, low toxicity, and is not easy to produce tolerance; it has good solubility, flexible dosage form, and convenient administration, so it is widely used in clinical practice. Since it was successfully developed in 1980, many scholars have conducted in-depth research on the pharmacological effects of ASA, and found that ASA not only has antimalarial effects, but also has immune regulation, anti-tumor, liver protection, anti-inflamm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K9/19A61K31/357A61K47/10A61K47/26A61K47/61A61P35/00B82Y5/00
CPCA61K9/1075A61K9/19A61K31/357A61K47/10A61K47/26A61K47/61A61P35/00B82Y5/00
Inventor 侯振清陈诗端范仲雄王衍戈邢驰坤
Owner XIAMEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com