Modified base aptamer for specifically targeting osteoarthritis synovial cells and application of modified base aptamer

A technology for osteoarthritis and synoviocytes, applied in the field of modified base aptamers, can solve the problems of uncertain binding force, inability to prove specificity, inapplicability to OA synoviocytes, etc., to reduce cumbersome steps, The screening process is simple and the effect of specificity enhancement
CN112094846AActive Publication Date: 2020-12-18SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
Publication Date
2020-12-18

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Abstract

The invention provides a modified base aptamer for specifically targeting osteoarthritis synovial cells. The aptamer has a nucleotide sequence as shown in SEQ ID NO.1 or 2. Through modifying an aptamer library, performing positive selection on the osteoarthritis synovial cells and performing negative reverse screening on various kinds of skeletal muscle derived cells, the modified base aptamer forspecifically targeting the osteoarthritis synovial cells is finally obtained. The synovial cell aptamer sequence is screened from the modified aptamer library; the screening process is simple and convenient; complicated steps of a traditional method are greatly reduced; due to the modified structure, the binding force with target cells is higher; the specificity of the synovial cell aptamer is specifically and greatly enhanced; the aptamer targeted binding defect of existing osteoarthritis disease derived synovial cells is overcome; and in the subsequent process, modified groups AminedU in the target aptamer can directly carry nanometer particles to deliver drugs, interfering RNA, miRNA, exosomes and the like to act on different target points to perform specific treatment on synovitis.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a modified base aptamer specifically targeting osteoarthritis synoviocytes and an application thereof. Background technique

[0002] Osteoarthritis (OA) is a common type of arthritis in the elderly population, which may eventually lead to the destruction and loss of joint function, seriously affecting the quality of life of the elderly, and is a heavy burden on individuals and society. The occult nature of early OA diseases and the complexity of differential diagnosis are also the reasons for the delay in treatment and poor prognosis of these diseases. The basic pathological features of OA are cartilage degeneration, subchondral bone hyperplasia, and synovitis; while persistent chronic synovitis, synovial membrane hyperplasia accompanied by bone invasion and destruction inflammation. In the above-mentioned synovitis diseases, since there are no blood vessels on the surface of articu...

Claims

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