Method for simultaneously detecting nine chemical components in quisqualis indica

A kind of chemical composition, gentleman's technology, applied in the field of analysis and detection, can solve the problem of difficult to reflect the overall quality and so on

Active Publication Date: 2020-12-22
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0003] However, at present, there are few research reports on the quality control of Shijunzi. The 2020 edition of "Pharmacopoeia of the People's Republic of China" (hereinafter referred to as "Chinese Pharmacopoeia") makes Trigonelline as the index for content determination of Junzi; Chen Zhihua et al. (Chen Zhihua, Li Menglu, Lv Weiqi, et al. Determination and correlation analysis of trigonelline content in the fruit of 18 producing areas of C. chinensis [J]. Zhejiang Journal of Integrated Traditional Chinese and Western Medicine, 2018, 28(11): 972-975.) HPLC method was used to determine the contents of C. chinensis in different producing areas Content of trigonelline in Chinese herbal medicine; Jiang Shunyao...

Method used

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  • Method for simultaneously detecting nine chemical components in quisqualis indica
  • Method for simultaneously detecting nine chemical components in quisqualis indica
  • Method for simultaneously detecting nine chemical components in quisqualis indica

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preparation example Construction

[0072] In the present invention, the preparation method of the linear curve preferably comprises the following steps:

[0073] The reference substance series solutions of 9 kinds of chemical components were prepared respectively; the 9 kinds of chemical components were trigonelline, malic acid, gallic acid, catechin, methyl gallate, vanillic acid, syringic acid, ellagic acid and arabic acid. ferulic acid;

[0074] The reference substance series solutions of the 9 chemical components were detected by UPLC-MS / MS to obtain the chromatographic peak areas of the 9 chemical components respectively, and the chromatographic peak areas were linearly fitted with the concentrations to obtain a linear curve.

[0075] The present invention prepares respectively the reference substance series solution of 9 kinds of chemical components; said 9 kinds of chemical components are trigonelline, malic acid, gallic acid, catechin, methyl gallate, vanillic acid, syringic acid, ellagic acid and feru...

Embodiment 1

[0099] (1) Preparation of reference substance series solutions

[0100] (1.1) prepare the reference substance mother liquor of 9 kinds of chemical components

[0101] Accurately weigh 36.45mg of trigonelline, add methanol to dissolve, transfer to a 25mL volumetric flask and set the volume to the mark, shake well, and obtain a concentration of 1.458mg / mL of trigonelline reference substance mother solution;

[0102] Accurately weigh 15.95mg of malic acid, add methanol to dissolve, transfer to a 25mL volumetric flask and set the volume to the mark, and shake well to obtain the mother solution of malic acid reference substance with a concentration of 1.458mg / mL;

[0103] Accurately weigh 12.25mg of gallic acid, add methanol to dissolve, transfer to a 25mL volumetric flask and set the volume to the mark, and shake well to obtain a gallic acid mother solution with a concentration of 0.49mg / mL;

[0104] Accurately weigh 1.175mg of catechin, add methanol to dissolve, transfer to a 25...

Embodiment 2

[0150] precision test

[0151]With the mixed reference substance solution prepared in embodiment 1 step (1.2), adopt the UPLC-MS / MS detection condition of embodiment 1 step (3.1) to carry out UPLC-MS / MS detection, record the peak area of ​​9 kinds of reference substances, within 1 day The intra-day precision was tested 6 times in a row; the inter-day precision was tested 3 times a day for 3 consecutive days. According to the linear regression equation in Table 4, the concentrations of the nine chemical components in the mixed reference solution were calculated, and the intra-day precision and inter-day precision were calculated through the peak concentrations of each chemical component. The results are shown in Table 5.

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Abstract

The invention provides a method for simultaneously detecting nine chemical components in quisqualis indica, and relates to the technical field of analysis and detection. The method comprises the following steps: extracting quisqualis indica by adopting an alcohol-water solution to obtain a to-be-detected sample solution; and then performing UPLC-MS/MS. According to the invention, a to-be-detectedquisqualis indica sample is pretreated through a UPLC MS/MS method, qualitative identification can be rapidly achieved while gradient elution conditions are controlled, nine chemicals (trigonelline, vanillic acid, ferulic acid, syringic acid, catechinic acid, ellagic acid, malic acid, gallic acid and methyl gallate) in the quisqualis indica medicinal material are quantitatively detected, and reliable experimental data are provided for development and utilization and quality evaluation of the quisqualis indica medicinal material. The detection method provided by the invention has the advantagesof simple operation, fast analysis time of only 8min, fast separation speed and high sensitivity, makes up the blank of nine chemical detection methods in quisqualis indica at present, and is worthyof popularization and application.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a method for simultaneously detecting 9 chemical components in the genie. Background technique [0002] Shijunzi is the dry and ripe fruit of Shijunzi, a plant of the Genusiaceae family, Shijunzi. It is sweet and warm in nature, and returns to the stomach and spleen meridian. Its functions and indications are: killing insects and eliminating accumulation, strengthening the spleen and stomach, removing asthenic heat, and killing visceral insects. Modern pharmacological studies have shown that Shijunzi has various pharmacological effects such as antibacterial, antiviral, insecticidal, anti-oxidative, anti-inflammatory, anti-hyperlipidemia, anti-diabetic, and anti-cancer. The research on the chemical components of Shijunzi shows that it mainly includes organic acids, tannins, volatile compounds, etc., and the multi-components are the material basis of the various phar...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/88G01N30/06Y02A50/30
Inventor 许文温秀萍徐伟
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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