Method for simultaneously detecting nine chemical components in quisqualis indica

Abstract

The invention provides a method for simultaneously detecting nine chemical components in quisqualis indica, and relates to the technical field of analysis and detection. The method comprises the following steps: extracting quisqualis indica by adopting an alcohol-water solution to obtain a to-be-detected sample solution; and then performing UPLC-MS / MS. According to the invention, a to-be-detectedquisqualis indica sample is pretreated through a UPLC MS / MS method, qualitative identification can be rapidly achieved while gradient elution conditions are controlled, nine chemicals (trigonelline, vanillic acid, ferulic acid, syringic acid, catechinic acid, ellagic acid, malic acid, gallic acid and methyl gallate) in the quisqualis indica medicinal material are quantitatively detected, and reliable experimental data are provided for development and utilization and quality evaluation of the quisqualis indica medicinal material. The detection method provided by the invention has the advantagesof simple operation, fast analysis time of only 8min, fast separation speed and high sensitivity, makes up the blank of nine chemical detection methods in quisqualis indica at present, and is worthyof popularization and application.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a method for simultaneously detecting 9 chemical components in the genie. Background technique [0002] Shijunzi is the dry and ripe fruit of Shijunzi, a plant of the Genusiaceae family, Shijunzi. It is sweet and warm in nature, and returns to the stomach and spleen meridian. Its functions and indications are: killing insects and eliminating accumulation, strengthening the spleen and stomach, removing asthenic heat, and killing visceral insects. Modern pharmacological studies have shown that Shijunzi has various pharmacological effects such as antibacterial, antiviral, insecticidal, anti-oxidative, anti-inflammatory, anti-hyperlipidemia, anti-diabetic, and anti-cancer. The research on the chemical components of Shijunzi shows that it mainly includes organic acids, tannins, volatile compounds, etc., and the multi-components are the material basis of the various phar...

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Problems solved by technology

[0003] However, at present, there are few research reports on the quality control of Shijunzi. The 2020 edition of "Pharmacopoeia of the People's Republic of China" (hereinafter referred to as "Chinese Pharmacopoeia") makes Trigonelline as the index for content determination of Junzi; Chen Zhihua et al. (Chen Zhihua, Li Menglu, Lv Weiqi, et al. Determination and correlation analysis of trigonelline content in the fruit of 18 producing areas of C. chinensis [J]. Zhejiang Journal of Integrated Traditional Chinese and Western Medicine, 2018, 28(11): 972-975.) HPLC method was used to determine the contents of C. chinensis in different producing areas Content of trigonelline in Chinese herbal medicine; Jiang Shunyao et al. (Jiang Shunyao, Tian Songjiu. Analysis of Trigonelline in Herbal Herbs by High Performance Ion Exchange Chromatography[J]. Chinese Journal of Traditional Chinese Medicine, 2004,29(2):44-46 +96.) Analysis of Trigonelline in Herbs of Junzi by High Performance Ion Exchange Chromatography

However, the above-mentioned prior art only detects the trigonelline component of Gentleman, which is difficult to reflect the overall quality of the various pharmacological effects of Gentleman.

At present, there is no literature report on the determination of the multi-components of Junzi, and the establishment of a multi-component comprehensive evaluation method is of great significance for improving the quality control of Junzi

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