Casein calcium phosphorus microsphere collagen filling agent, preparation method of filling agent and preparation method of casein calcium phosphorus microspheres

A technology of calcium caseinate and collagen, which is applied in the field of medical materials, can solve the problems of ineffective stimulation of collagen repair and short maintenance time, and achieve the effect of improving effectiveness and maintenance time, improving effectiveness, and low preparation cost

Pending Publication Date: 2020-12-25
SHANGHAI MOYANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current collagen fillers generally have a short maintenance time and cannot effectively stimulate the repair of autologous collagen, so there is huge room for improvement

Method used

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  • Casein calcium phosphorus microsphere collagen filling agent, preparation method of filling agent and preparation method of casein calcium phosphorus microspheres

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0040] The present invention also proposes a preparation method of casein calcium phosphorus microsphere collagen filler, comprising the following steps:

[0041] Step 1: Select collagen. As the collagen, select animal source type I collagen without atelopeptide and animal source type III collagen without atelopeptide, and proceed to step 2 and step 3;

[0042] When the collagen chooses atelopeptide animal source type I collagen, atelopeptide animal source type III collagen, genetically recombinant human type I collagen and genetically recombinant human type III collagen, skip step 2 and step 3, Go to step 4 directly.

[0043] Step 2: Rinse the collagen without atelopeptide with purified water, add 100ml of 0.1-1.0mol / L acetic acid solution for every 1-10g of collagen, and then add 1-10mL of specific pepsin for stirring and hydrolysis to remove the telogen Peptide; the stirring speed is 50-1000RPM, the temperature is 10-30℃, and the hydrolysis time is 2-36h.

[0044] Step 3:...

Embodiment 1

[0056] Embodiment 1: a kind of casein calcium phosphorus microsphere collagen filler, the specific preparation method is as follows:

[0057] 1) Prepare 2 L of 0.3 mol / L calcium nitrate solution, add 2 g of casein to dissolve and mix well, add ammonia water to adjust to pH = 8.0, and obtain the first solution;

[0058] 2) Prepare 2L of 0.252mol / L diammonium hydrogen phosphate solution, adjust to pH=8.0, add the first solution for mixing, stir, adjust pH=8.0, react at 10°C for 48h, use 0.1 The μm membrane is suction-filtered to extract the precipitate to obtain a filter cake to obtain the first precipitate, wherein the stirring speed is 50rpm;

[0059] 3) Wash the filter cake formed by the first precipitate with 4L of purified water, then wash with 2L of absolute ethanol, and centrifuge to obtain the second precipitate after washing;

[0060] 4) Prepare 1 L of casein aqueous solution with a concentration of 10 mg / mL as a buffer, and resuspend the second precipitate using the b...

Embodiment 2

[0069] Embodiment 2: a kind of casein calcium phosphorus microsphere collagen filler, the specific preparation method is as follows:

[0070] 1) Prepare 5L of 3moL / L calcium nitrate solution, add 100g of casein to dissolve and mix well, add ammonia water to adjust to pH=10.5, and obtain the first solution;

[0071] 2) Prepare 5L of 5.04moL / L ammonium dihydrogen phosphate solution, adjust to pH = 10.5, add the first solution for mixing, stir, adjust pH = 10.5, react at 90°C for 12 hours, use the dish after the reaction is completed A disc centrifuge extracts the precipitate to obtain the first precipitate, wherein the stirring speed is 800rpm, and the disc centrifuge rotates at 300rpm;

[0072] 3) Wash the filter cake formed by the first precipitate with 12L of purified water, then wash with 8L of absolute ethanol, and centrifuge to obtain the second precipitate after washing;

[0073] 4) Prepare 2.5 L of casein aqueous solution with a concentration of 200 mg / mL as a buffer, a...

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Abstract

The invention provides a casein calcium phosphorus microsphere collagen filling agent, a preparation method of the filling agent and a preparation method of casein calcium phosphorus microspheres. Thecasein calcium phosphorus microspheres as a novel material are adopted, so that the effectiveness and the maintaining time of a traditional collagen filling agent are improved and prolonged, the biological safety is high, and expression of collagen can be stimulated; and the casein calcium phosphorus microspheres have extremely high application prospect and value. Terminal peptide-free animal collagen or genetic recombinant human collagen is used, the material immunogenicity is low, and the material purchasing / preparing cost is low, so that the collagen is suitable for large-scale industrialproduction. Auxiliary components such as glycerin or small molecular hyaluronic acid are added into the filling agent, so that the rheological property of the filling agent can be improved, and the filling agent has stronger filling performance and is easier to inject. The process provided by the invention has the advantages of wide application prospect, low cost, no harmful wastes, industrial production, high practicability and the like.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a casein calcium phosphorus microsphere collagen filler, a preparation method thereof and a preparation method of the casein calcium phosphorus microsphere. Background technique [0002] Casein is an amphipathic phosphoprotein, including a large number of glutamic acid and aspartic acid residues and a small amount of phosphorylated serine residues. Porous microspheres prepared by casein micelles and inorganic calcium and phosphorus components , can combine shape advantages and material advantages, and has broad application prospects in tissue engineering and medical fields. [0003] Collagen is a commonly used component of medical fillers, among which atelopeptide animal collagen or genetically recombinant human collagen is more suitable for use in the field of medical fillers due to its lower immunogenicity. However, the current collagen fillers generally have a short...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/22A61L27/24A61L27/60
CPCA61L27/227A61L27/24A61L27/50A61L27/60A61L2400/06A61L2430/02A61L2430/32
Inventor 林光明秦照宇江春阳
Owner SHANGHAI MOYANG BIOTECHNOLOGY CO LTD
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