Preparation method and application of red fluorescent gold nanocluster and targeting complex thereof

A gold nanocluster, red fluorescence technology, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of few reports of functionalized fluorescent AuNCs and complex synthesis process, and achieve broad application prospects and fluorescence properties. Stable and good water solubility

Inactive Publication Date: 2020-12-29
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the synthesis process of fluorescent AuNCs is relatively complicated, and there are few reports on functionalized fluorescent AuNCs.

Method used

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  • Preparation method and application of red fluorescent gold nanocluster and targeting complex thereof
  • Preparation method and application of red fluorescent gold nanocluster and targeting complex thereof
  • Preparation method and application of red fluorescent gold nanocluster and targeting complex thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of red fluorescent gold nanoclusters (AuNCs@Lzm, AuL):

[0035] (1) (4mM, 107.1μL) tetrachloroauric acid aqueous solution and (7.14x10 -2 mM, 1mL) lysozyme aqueous solution was added to the microwave tube, and stirred for 5min;

[0036] (2) Transfer the mixed solution obtained in step (1) to a microwave reactor, and react with microwave at 50° C. for 5 minutes;

[0037] (3) Dialyzing the product obtained in step (2) with a dialysis bag with a molecular weight cut-off of 8,000 to 14,000 Da for 48 hours to finally obtain a red fluorescent gold nanocluster solution;

[0038] (4) Further making red fluorescent gold nanocluster powder.

Embodiment 2

[0040] Morphological characterization of red fluorescent gold nanoclusters:

[0041] A small amount of dry red fluorescent gold nanocluster powder prepared was dispersed in water, dropped onto a copper grid, and after drying, the morphology of the sample was analyzed by a transmission electron microscope.

[0042] The electron microscope images of the prepared red fluorescent gold nanoclusters are shown in figure 1 , it can be seen from the figure that the red fluorescent gold nanoclusters are spherical, and the lattice spacing is 0.25nm, indicating that it is similar to the (111) crystal plane of face-centered cubic (fcc) Au / Ag, with a value of 0.23nm.

Embodiment 3

[0044] Fluorescence Spectrometry of Red Fluorescent Gold Nanoclusters:

[0045] Take 1 mL of the red fluorescent gold nanocluster solution and add it into the fluorescent cup, and measure the fluorescence spectrum of the cluster with a fluorometer.

[0046] The fluorescence spectra of the prepared red fluorescent gold nanoclusters are shown in image 3 , it can be seen from the figure that the optimal excitation wavelength of the cluster is 380nm, and the optimal emission wavelength is 650nm, which is located in the near-infrared region and avoids the interference of intracellular autofluorescence. The inset is a picture taken with UV irradiation (365 nm).

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Abstract

The invention provides a preparation method and application of a red fluorescence gold nanocluster and a targeting complex thereof. According to the invention, lysozyme with good biocompatibility is used as a protective agent and a reducing agent, and reacts with tetrachloroauric acid through a microwave method to synthesize a red fluorescence gold nanocluster, and the prepared gold nanocluster and hyaluronic acid are self-assembled through a physical adsorption effect so as to obtain a targeting gold nanocluster complex, wherein the complex can successfully recognize tumor cells in a targeting manner; and the prepared red fluorescence gold nanocluster is good in water solubility, stable in fluorescence property, excellent in biocompatibility and low in toxicity, and has wide application prospects in the aspects of bioimaging, biomarkers and tumor recognition.

Description

technical field [0001] The invention relates to nanomaterials and targeting complexes, in particular to a preparation method and application of red fluorescent gold nanoclusters and targeting complexes. Background technique [0002] In recent years, fluorescent gold nanoclusters (AuNCs), as promising new fluorescent nanomaterials, have attracted much attention due to their unique physicochemical properties and favorable optical properties. As reported in the literature, fluorescent AuNCs usually consist of a few to hundreds of Au atoms with a size smaller than 2 nm, which is comparable to the Fermi wavelength of electrons. Unlike large gold nanoparticles (AuNPs), ultrasmall fluorescent AuNCs do not show surface plasmon resonance absorption in the visible region, but possess significant fluorescence emission in the near-infrared (NIR) in the visible region. By designing and controlling the size, AuNCs can have excellent properties such as large Stokes shift, quantum confinem...

Claims

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Application Information

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IPC IPC(8): C09K11/58C09K11/06G01N21/64
CPCC09K11/06C09K11/58C09K2211/188G01N21/6428G01N2021/6439
Inventor 李英奇李文艳
Owner SHANXI UNIV
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