Preparation method and application of red fluorescent gold nanocluster and targeting complex thereof
A gold nanocluster, red fluorescence technology, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of few reports of functionalized fluorescent AuNCs and complex synthesis process, and achieve broad application prospects and fluorescence properties. Stable and good water solubility
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Embodiment 1
[0034] Preparation of red fluorescent gold nanoclusters (AuNCs@Lzm, AuL):
[0035] (1) (4mM, 107.1μL) tetrachloroauric acid aqueous solution and (7.14x10 -2 mM, 1mL) lysozyme aqueous solution was added to the microwave tube, and stirred for 5min;
[0036] (2) Transfer the mixed solution obtained in step (1) to a microwave reactor, and react with microwave at 50° C. for 5 minutes;
[0037] (3) Dialyzing the product obtained in step (2) with a dialysis bag with a molecular weight cut-off of 8,000 to 14,000 Da for 48 hours to finally obtain a red fluorescent gold nanocluster solution;
[0038] (4) Further making red fluorescent gold nanocluster powder.
Embodiment 2
[0040] Morphological characterization of red fluorescent gold nanoclusters:
[0041] A small amount of dry red fluorescent gold nanocluster powder prepared was dispersed in water, dropped onto a copper grid, and after drying, the morphology of the sample was analyzed by a transmission electron microscope.
[0042] The electron microscope images of the prepared red fluorescent gold nanoclusters are shown in figure 1 , it can be seen from the figure that the red fluorescent gold nanoclusters are spherical, and the lattice spacing is 0.25nm, indicating that it is similar to the (111) crystal plane of face-centered cubic (fcc) Au / Ag, with a value of 0.23nm.
Embodiment 3
[0044] Fluorescence Spectrometry of Red Fluorescent Gold Nanoclusters:
[0045] Take 1 mL of the red fluorescent gold nanocluster solution and add it into the fluorescent cup, and measure the fluorescence spectrum of the cluster with a fluorometer.
[0046] The fluorescence spectra of the prepared red fluorescent gold nanoclusters are shown in image 3 , it can be seen from the figure that the optimal excitation wavelength of the cluster is 380nm, and the optimal emission wavelength is 650nm, which is located in the near-infrared region and avoids the interference of intracellular autofluorescence. The inset is a picture taken with UV irradiation (365 nm).
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