Determination method of nucleoside content in Poria cocos
A detection method and nucleoside technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of low content, and achieve the effects of high detection efficiency, reduced errors, and strong practicability.
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Embodiment 1
[0076] 1. The conditions of the HPLC detection adopted in the present embodiment are as follows: Zorbax SB-Aq chromatographic column (5 μm, 4.6 × 250mm, Agilent), detection wavelength: 260nm, column temperature 30°C, flow rate: 0.8mL / min, Injection volume: 10 μL, the mobile phase is acetonitrile and water, and the gradient elution conditions are shown in Table 2 below:
[0077] Table 2 gradient elution conditions
[0078] time / min water / % Acetonitrile / % 0～12 100 0 12～20 100→98 0→2 20～45 98→80 2→20 45～46 80→100 20→0 46～60 100 0
[0079] The meaning of percent sign in the table is volume percent.
[0080] 2. Preparation of internal standard solution
[0081] Accurately weigh geniposide, place it in a 10mL volumetric flask, add methanol to dissolve to a constant volume, and prepare a 0.6mg / mL internal standard solution.
[0082] 3. Preparation of mixed reference solution containing internal standard
[0083] Accurately measure th...
Embodiment 2
[0125] The conditions for the determination of liquid chromatography adopted in this comparative example are as follows: Zorbax SB-Aq chromatographic column (5 μm, 4.6 × 250mm, Agilent), detection wavelength 260nm, column temperature 30 ℃, flow rate 0.5mL / min, injection volume: 10 μL, the mobile phase is acetonitrile and water, the gradient elution conditions of this comparative example are shown in the following table 9:
[0126] Table 9 gradient elution conditions
[0127] time / min Acetonitrile / % water / % 0～5 0→5 100→95 5～10 5 95 10～20 5→20 95→80 20～25 20 80 25～26 20→0 80→100 26～40 0 100
[0128] The meaning of percent sign in the table is volume percent.
[0129] Prepare the Poria cocos need testing solution that does not contain internal standard according to the method of embodiment 1, detect by the gradient elution condition of table 9, gained chromatogram is as follows Figure 4 shown. It can be seen from the figure...
Embodiment 3
[0131] The conditions for the determination of the liquid chromatography method used in this comparative example are as follows: Zorbax SB-Aq chromatographic column (5 μ m, 4.6 × 250 mm, Agilent), detection wavelength: 260 nm, column temperature 30 ° C, flow rate: 0.5 mL / min, sample injection Volume: 10 μL, mobile phase: acetonitrile and 0.05% formic acid / water solution (V / V), the gradient elution conditions of this comparative example are shown in Table 9 below:
[0132] Table 10 gradient elution conditions
[0133] time / min Acetonitrile / % 0.05% formic acid / water 0～12 0 100 12～17 0→2 100→98 17～27 2 98 27～45 2→20 98→80
[0134] The meaning of percent sign in the table is volume percent.
[0135] Prepare the Poria cocos need testing solution that does not contain internal standard according to the method of embodiment 1, detect by the gradient elution condition of table 10, gained chromatogram is as follows image 3 shown. It can be s...
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