Composite nanoprobe with targeted fluorescence/magnetic resonance bimodal imaging and photothermal therapy functions as well as preparation and application of composite nanoprobe

A dual-modality imaging and photothermal therapy technology, applied in the fields of nanomaterials and biomedicine, can solve the problems of inability to specifically identify tumor cells, poor stability of components, poor consistency, and inability to meet research and application requirements. Excellent photothermal stability, obvious killing effect of photothermal therapy, good biocompatibility and fluorescence stability

Active Publication Date: 2021-01-01
XUZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the photothermal therapy nanosystem prepared by the existing process has poor stability and consistency of components, poor biocompatibility, cannot specifically identify tumor cells, and the preparation method is complicated, which cannot meet the needs of research and application.

Method used

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  • Composite nanoprobe with targeted fluorescence/magnetic resonance bimodal imaging and photothermal therapy functions as well as preparation and application of composite nanoprobe
  • Composite nanoprobe with targeted fluorescence/magnetic resonance bimodal imaging and photothermal therapy functions as well as preparation and application of composite nanoprobe
  • Composite nanoprobe with targeted fluorescence/magnetic resonance bimodal imaging and photothermal therapy functions as well as preparation and application of composite nanoprobe

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Embodiment 1

[0051] The present invention first adopts the seed growth method to prepare the gold nano biconical AuNBP dispersion liquid, and purifies the AuNBP to obtain pure AuNBP nanoparticles; then uses sodium borohydride to reduce the disulfide bond in the BSA molecule into A mercapto group with a strong coordination effect is obtained to obtain denatured BSA (denatured BSA, dBSA), and it is modified on the surface of AuNBP; then the dBSA on the surface of AuNBP is used as a stabilizer to prepare Gd 2 o 3 Nanoparticles and gold nanoclusters to obtain a nanocomposite AuNBP-Gd with functions of fluorescence / magnetic resonance dual-modality imaging and photothermal therapy 2 o 3 / Au-dBSA. Coupling targeting recognition molecule AS1411 aptamer to form targeting nanoprobe AuNBP-Gd 2 o 3 / Au-dBSA-AS1411, the preparation process is as follows figure 1 shown. The specific implementation process is as follows:

[0052] S1. Preparation of AuNBP

[0053] First prepare Au nano-seeds, add ...

Embodiment 2

[0078] For further verification of AuNBP-Gd 2 o 3 / Au-dBSA-AS1411 targeting nanoprobe application in tumor diagnosis and treatment, in vitro cell experiments. Among them, human breast cancer cells (MDA-MB-231) and human skin fibroblasts (HSF) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences.

[0079] 1. Cytotoxicity Test

[0080] Evaluation of AuNBP-Gd by CCK8 method 2 o 3 / Au-dBSA Toxic Effects on Human Breast Cancer Cells (MDA-MB-231) and Human Skin Fibroblasts (HSF). The MDA-MB-231 cells and HSF cells in the logarithmic growth phase were inoculated in two 96-well culture plates respectively, and the inoculation concentration was about 0.5×10 5 cells / mL, put in 37℃, 5%CO 2 Incubate in the incubator for 24h. When it is observed under an inverted microscope that the cells are about to fill the culture wells, discard the old culture medium, wash it twice with sterilized PBS solution, divide the 96-well plate into 6 groups (6 wells / group), and...

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Abstract

The invention discloses a composite nanoprobe with targeted fluorescence/magnetic resonance bimodal imaging and photothermal therapy functions, as well as a preparation and application of the composite nanoprobe. The preparation method comprises the following steps of firstly, preparing AuNBP by adopting a seed growth method, separating and purifying the AuNBP to obtain pure AuNBP nanoparticles, and then modifying the surfaces of the AuNBP nanoparticles with sulfhydrylated BSA; modifying Gd2O3 nanoparticles and Au nanoclusters on the surfaces of AuNBP nanoparticles with the modified AuNBP nanoparticles as a stabilizer to obtain an AuNBP-Gd2O3/Au-dBSA composite nanomaterial; and finally enabling an aptamer AS1411 to be connected to the surface of AuNBP-Gd2O3/Au-dBSA to obtain the AuNBP-Gd2O3/Au-dBSA-AS1411 targeted composite nanoprobe. The AuNBP-Gd2O3/Au-dBSA-AS1411 composite nanoprobe provided by the invention has good biocompatibility, fluorescence stability and photothermal stability, also has fluorescence/magnetic resonance bimodal imaging and photothermal therapy functions, can enter tumor cells in a targeting manner, marks and traces the tumor cells, and is beneficial to earlydiagnosis and treatment of tumors.

Description

technical field [0001] The invention relates to the technical fields of nanomaterials and biomedicine, in particular to a composite nanoprobe with the functions of targeted fluorescence / magnetic resonance dual-mode imaging and photothermal therapy, and its preparation and application. Background technique [0002] Molecular imaging is a science that uses imaging technology to non-invasively detect molecules involved in physiological or pathological processes in vivo, and conduct qualitative and quantitative research on changes in molecules, genes and cells in vivo. Among various imaging techniques, magnetic resonance imaging (MRI) is a non-invasive imaging technique with high soft tissue resolution and spatial resolution, capable of providing multi-sequence, multi-parameter and multi-directional imaging, but its sensitivity is relatively low , fluorescence imaging technology has a very high sensitivity, but its penetration is limited, so the combination of magnetic resonance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/14A61K49/18A61K41/00A61P35/00A61P15/14
CPCA61K49/0056A61K49/0093A61K49/143A61K49/1869A61K41/0052A61P35/00A61P15/14
Inventor 庄银苹王永盖新亭李梦双陈莹徐凯吴长宇郑绍辉李菁菁韩翠平
Owner XUZHOU MEDICAL UNIV
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