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Preparation method of polydopamine mediated magnetic bimetallic nano-enzyme

A technology of bimetallic nano and polydopamine, applied in chemical synthesis and nano material fields, to achieve high catalytic activity, excellent stability and biocompatibility, good stability and biocompatibility

Pending Publication Date: 2021-01-12
江西维邦生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Based on the foregoing, the inventors have developed a polydopamine-mediated magnetic bimetallic (palladium / platinum) nanozyme (Fe 3 o 4 @PDA@Pd / Pt), currently, there is no Fe 3 o 4 Related reports on the preparation and application of @PDA@Pd / Pt

Method used

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  • Preparation method of polydopamine mediated magnetic bimetallic nano-enzyme
  • Preparation method of polydopamine mediated magnetic bimetallic nano-enzyme
  • Preparation method of polydopamine mediated magnetic bimetallic nano-enzyme

Examples

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Embodiment 1

[0025] Embodiment 1: 100nm particle diameter Fe 3 o 4 Preparation of @PDA@Pd / Pt

[0026] 1. Preparation of Fe 3 o 4 : Weigh ferric chloride, polyethylene glycol-3000 and sodium acetate and dissolve them in ethylene glycol so that the final concentrations are 10, 15, and 60 mg / mL respectively, and react in a sealed autoclave at 200°C for 12 hours. After the reaction, magnetic Separated, washed 3 times with water, dried to obtain Fe 3 o 4 ;

[0027] 2. Preparation of Fe 3 o 4 @PDA: take Fe3 o 4 Disperse in Tris-HCl buffer solution (0.05M, pH=8.5) to make the final concentration 50μg / mL, add dopamine hydrochloride (10mg / mL) to make the final concentration 50μg / mL, and stir the reaction in the dark (250r / min) 15h, magnetically separated after the reaction, washed three times with water and redissolved in water to obtain Fe 3 o 4 @PDA;

[0028] 3. Preparation of Fe 3 o 4 @PDA@Pd / Pt: Take a certain amount of Fe 3 o 4 @PDA was dispersed in polyvinylpyrrolidone soluti...

Embodiment 2

[0029] Embodiment 2: 200nm particle diameter Fe 3 o 4 Preparation of @PDA@Pd / Pt

[0030] 1. Preparation of Fe 3 o 4 : Weigh ferric chloride, polyethylene glycol-4000 and sodium acetate and dissolve them in ethylene glycol so that the final concentrations are 20, 15, and 60 mg / mL respectively, and react in a sealed autoclave at 200°C for 16 hours. After the reaction, magnetic Separated, washed 3 times with water, dried to obtain Fe 3 o 4 ;

[0031] 2. Preparation of Fe 3 o 4 @PDA: take Fe 3 o 4 Disperse in Tris-HCl buffer solution (0.05M, pH=8.5) to make the final concentration 50μg / mL, add dopamine hydrochloride (10mg / mL) to make the final concentration 100μg / mL, and stir the reaction in the dark (250r / min) 20h, magnetically separated after the reaction, washed three times with water and redissolved in water to obtain Fe 3 o 4 @PDA;

[0032] 3. Preparation of Fe 3 o 4 @PDA@Pd / Pt: Take a certain amount of Fe 3 o 4 @PDA was dispersed in polyvinylpyrrolidone sol...

Embodiment 3

[0033] Example 3: 1 μm particle size Fe 3 o 4 Preparation of @PDA@Pd / Pt

[0034] 1. Preparation of Fe 3 o 4 : Weigh ferric chloride, polyethylene glycol-10000 and sodium acetate and dissolve them in ethylene glycol so that the final concentrations are 40, 35, and 80 mg / mL respectively, and react in a sealed autoclave at 200°C for 20 hours. After the reaction, magnetic Separated, washed 3 times with water, dried to obtain Fe 3 o 4 ;

[0035] 2. Preparation of Fe 3 o 4 @PDA: take Fe 3 o 4 Disperse in Tris-HCl buffer solution (0.05M, pH=8.5) to make the final concentration 200μg / mL, add dopamine hydrochloride (50mg / mL) to make the final concentration 500μg / mL, and stir the reaction in the dark (250r / min) 30h, magnetically separated after the reaction, washed three times with water and redissolved in water to obtain Fe 3 o 4 @PDA;

[0036] 3. Preparation of Fe 3 o 4 @PDA@Pd / Pt: Take a certain amount of Fe 3 o 4 @PDA was dispersed in polyvinylpyrrolidone solution ...

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Abstract

The invention discloses a preparation method of a polydopamine mediated magnetic bimetallic nano-enzyme. The preparation method comprises the following steps: taking ferric trichloride as an iron source, preparing ferroferric oxide nanoparticles through a solvothermal method, coating the surfaces of the ferroferric oxide nanoparticles with a polydopamine thin layer, mediating sodium chloropalladate and potassium chloroplatinate for in-situ reduction, and enabling palladium / platinum to grow on the surface of the polydopamine thin layer, thereby obtaining the magnetic bimetallic nano-enzyme. According to the preparation method, ferroferric oxide is used as a magnetic core to endow the nano-enzyme with magnetism, so that the recycling of the nano-enzyme is realized, rich palladium / platinum growth sites can be provided through polydopamine mediated growth of palladium / platinum, the prepared nano-enzyme has excellent stability and biocompatibility, and meanwhile, due to the synergistic effect of palladium / platinum double metals, the prepared nano-enzyme has excellent enzyme catalytic activity.

Description

technical field [0001] The invention belongs to the field of chemical synthesis and nanomaterials, in particular to a polydopamine-mediated magnetic bimetallic (palladium / platinum) nanozyme (Fe 3 o 4 @PDA@Pd / Pt) preparation method. Background technique [0002] Enzymes are proteins or RNAs with potent catalytic activity and high specificity for their substrates. Enzyme-catalyzed reaction is a type of chemical reaction that reduces the reaction energy through the participation of enzymes, so that the reaction can proceed normally. The characteristic of this reaction is that only a very small amount of enzyme is required to produce a large number of reaction products, so it is widely used in the fields of medicine, biology and chemistry. However, due to their structural properties, traditional enzymes (proteins and RNAs) often have insufficient stability and strict catalytic conditions. Nanozymes have attracted more and more attention from the industry due to their high sta...

Claims

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Application Information

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IPC IPC(8): B01J31/06B01J23/89B01J31/28B82Y30/00B82Y40/00
CPCB01J31/069B01J23/8906B01J31/28B01J31/003B82Y30/00B82Y40/00B01J35/33
Inventor 赖卫华熊勇华章钢刚彭娟李响敏刘文娟伍燕华
Owner 江西维邦生物科技有限公司
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