Lactobacillus rhamnosus LRa05 for antagonizing shigella, screening method and application of lactobacillus rhamnosus LRa05

Abstract

The invention discloses lactobacillus rhamnosus LRa05, and the lactobacillus rhamnosus is collected in China General Microbiological Culture Collection Center, and the collection number is CGMCC 1.12734; the antagonistic ability of the lactobacillus rhamnosus has a good antagonistic effect on shigella, and the antagonistic ability of the lactobacillus rhamnosus is superior to that of common lactobacillus and commercial strain lactobacillus rhamnosus LGG; moreover, the lactobacillus rhamnosus has the characteristics of good acid resistance and bile tolerance; Animal test and human body test results show that a health-care food prepared from the lactobacillus rhamnosus LRa05 can effectively relieve symptoms including abdominal pain, diarrhea, fever and the like caused by the shigella; and besides, the lactobacillus rhamnosus belongs to probiotics, has been already incorporated into safe strains which can be applied to a food list directory, is excellent in safety, and can replace traditional antibiotics for use.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to Lactobacillus rhamnosus LRa05 antagonizing Shigella, a screening method and application thereof. Background technique [0002] Shigella Castellani (Shigella Castellani) is a kind of Gram-negative short bacillus, which is the most common pathogen of human bacillary dysentery. It belongs to Enterobacteriaceae and is divided into four groups: Shigella dysenteriae, Shigella flexneri bacilli, Shigella baumannii, and Shigella sonnei. No spores, no capsule, no flagella, most with pili. Enteropathogenic bacteria in humans and primates, causing bacillary dysentery. Every year, 200 million people worldwide are infected with shigella, and millions of people need to be hospitalized every year, and a large number of fatal cases occur in developing countries. It can kill as many as half a million people each year. Shigella-induced dysentery is primarily due to disruption of the intestinal...

AI Technical Summary

Problems solved by technology

Lactic acid bacteria are a general term for bacteria that can ferment carbohydrates to produce a large amount of lactic acid. They are widely found in naturally fermented dairy products, fermented traditional foods and the human intestine. Lactic acid bacteria can regulate the balance of intestinal flora, control endotoxins, and inhibit intestinal pathogenic The growth and reproduction of pathogens can have beneficial effects on the nutritional status, physiological functions, drug effects, immune responses, etc. of the body. It belongs to the beneficial bacteria in the intestinal tract, antagonizes the growth of other pathogenic bacteria, has no toxic side effects, and does not produce However, due to the different physiological, biochemical and antibacterial properties of different strains, there is currently no screening, identification and application of lactic acid bacteria that specifically antagonize Shigella

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