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Method for extracting purified phospholipid from clams

A clam and phospholipid technology, applied in the direction of chemical instruments and methods, edible phospholipid composition, silicon oxide, etc., to achieve the effect of convenient quality and reliable purification and extraction

Active Publication Date: 2021-01-22
ZHEJIANG GONGSHANG UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Typically, crude total lipids are extracted using organic solvents (including methanol, dichloromethane, chloroform, etc.), and the challenge is to purify impurities and low-abundance phospholipid molecular species in non-polar triglycerides MS

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  • Method for extracting purified phospholipid from clams
  • Method for extracting purified phospholipid from clams
  • Method for extracting purified phospholipid from clams

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Embodiment Construction

[0027] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but not as a basis for limiting the present invention.

[0028] Example.

[0029] 1.1 Materials and reagents

[0030] Graphite, 3-aminopropyltriethoxysilane (APTES) and tetraethylorthosilicate (TEOS) were purchased from SigmaAldrich (St. Louis, MO, USA). Acetonitrile and methanol were purchased from chromatographic grade Merck (Darmstadt, Germany). By supplying hydrogen peroxide (H 2 o 2 ), phosphoric acid (H 3 PO 4 ), hydrochloric acid (HCl), acetone, isopropanol, potassium permanganate (KMnO 4 ) and Urea Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Representative chemical standards of phosphatidylcholine (PC) 14:0 / 14:0, phosphatidylethanolamine (PE) 15:0 / 15:0 and phosphatidylinositol (PI) 16:0 / 16:0 were purchased from From Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Ultrapure water (18.2 MΩ·cm) was produced using a MilliQ system ...

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Abstract

The invention discloses a method for extracting purified phospholipid from clams. Themethod comprises the following steps of: (1) collecting clam tissues, homogenizing the clam tissues by using a high-speed dispersion machine, and performing drying under vacuum freezing to obtain clam powder; (2) carrying out ultrasonic treatment by using chloroform and methanol, and extracting crude lipid from the clam powder; (3) washing the crude lipid with cold acetone to obtain a crude lipid extract; and (4) performing adsorption extraction on phospholipid in the crude lipid extract by using a solid-phaseextraction method by taking a compound of silicon dioxide fiber nanoparticles and graphene oxide nanoparticles as an adsorbent. According to the method, highly purified phospholipid can be effectively and reliably extracted from the clams, so that lipidomics analysis and phenotypic analysis can be conveniently carried out.

Description

technical field [0001] The invention relates to a method for extracting and purifying phospholipids from clams, belonging to the technical field of food manufacturing and testing. Background technique [0002] Clams are shellfish, one of the most abundant marine molluscs, and are widely farmed and harvested worldwide. Asian clams (Corbicula afluminea) are mainly distributed in East Asian countries, especially China, Korea and Japan. The Food and Agriculture Organization of the United Nations (FAO) reports global capture and aquaculture production (2018) of over 6.6 million tonnes of clams, indicating that clams are the most productive species of shellfish. Due to its unique nutritional composition, clams are attractive to customers in coastal areas, and the huge consumption of clams has attracted extensive attention in the study of their nutritional composition, including minerals, lipids, proteins, etc. [0003] Recent studies have reported that clams are rich in polyunsa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/10B01J20/20B01J20/10C01B33/18C01B32/198B82Y30/00B82Y40/00G01N30/02G01N30/72G01N30/06G01N30/14
CPCC07F9/103B01J20/20B01J20/103C01B33/18C01B32/198B82Y30/00B82Y40/00G01N30/02G01N30/72G01N30/06G01N30/14C01P2004/16C01P2004/80G01N2030/062G01N2030/143G01N2030/065
Inventor 沈清宋恭帅王萍亚赵巧灵
Owner ZHEJIANG GONGSHANG UNIVERSITY