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A kind of production technology of tremella oligosaccharide

A production process, tremella technology, applied in the direction of sugar derivatives, sugar derivatives, sugar derivatives preparation, etc., can solve the problems of insoluble in water, large molecular weight, strong viscosity, etc., to reduce production environment requirements, composition The effect of simple, controllable degradation

Active Publication Date: 2022-03-08
FUJIAN GLOBAL OCEAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems in the prior art that tremella polysaccharides have huge molecular weight, complex structure, insoluble in water, strong viscosity and limited application range, the present invention discloses a production process of tremella oligosaccharides, the specific scheme is as follows:

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  • A kind of production technology of tremella oligosaccharide

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Embodiment 1

[0041] The preparation method of tremella oligosaccharide in the present embodiment, concrete steps are as follows:

[0042] Step 1: screening and culturing strains, preparing components and their concentrations are: tremella polysaccharide 2g / L, peptone 5g / L, yeast powder 1g / L, iron phosphate 0.01g / L, and the solvent is tap water, the pH of the mixture is =7.4, sterilize the culture medium at 121°C for 20 minutes, at a temperature of 28°C and a shaking speed of 180rpm, select rotten Tremella fungus for shaking culture in the culture liquid, dilute the shaken culture liquid by more than one dilution, and apply The components and their concentrations are as follows: tremella polysaccharide 15g / L, agarose 6g / L, peptone 5g / L, yeast powder 1g / L, iron phosphate 0.01g / L, and the solvent is tap water, and the pH of the mixture is = 7.4. Sterilize at 121°C for 20 minutes to screen the medium plate, make more than three parallel samples for each dilution, incubate the plate upside down...

Embodiment 2

[0047] The preparation method of tremella oligosaccharide in the present embodiment, concrete steps are as follows:

[0048] Step 1, strain screening and cultivation, preparation of each component and its concentration are: white fungus polysaccharide 1g / L, peptone 3g / L, yeast powder 0.5g / L, iron phosphate 0.01g / L, and the solvent is tap water, the mixed solution pH = 7.4, sterilized at 121°C for 20 minutes in the culture medium, at a temperature of 28°C and a shaking speed of 180rpm, select rotten white fungus and shake it in the culture medium, then dilute the shaken culture medium by more than one dilution Apply to each component and its concentration is: tremella polysaccharide 10g / L, agarose 6g / L, peptone 3g / L, yeast powder 0.5g / L, iron phosphate 0.01g / L, and the solvent is tap water, the mixed solution pH = 7.4, sterilize at 121°C for 20 minutes on the plate of the screening medium, make more than three parallel samples for each dilution, and culture the plate upside dow...

Embodiment 3

[0053] The preparation method of tremella oligosaccharide in the present embodiment, concrete steps are as follows:

[0054] Step 1, strain screening and cultivation, preparation of each component and its concentration are: tremella polysaccharide 3g / L, peptone 8g / L, yeast powder 2.5g / L, iron phosphate 0.02g / L, and the solvent is tap water, the mixed solution pH = 7.4, sterilized at 121°C for 20 minutes in the culture medium, at a temperature of 28°C and a shaking speed of 180rpm, select rotten white fungus and shake it in the culture medium, then dilute the shaken culture medium by more than one dilution Apply to each component and its concentration is: tremella polysaccharide 20g / L, agarose 9g / L, peptone 8g / L, yeast powder 2.5g / L, iron phosphate 0.02g / L, and the solvent is tap water, the mixed solution pH = 7.4, sterilize at 121°C for 20 minutes on the plate of the screening medium, make more than three parallel samples for each dilution, and culture the plate upside down at...

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Abstract

The invention discloses a production process of tremella oligosaccharides, which relates to the field of sugar compound processing. Screening and purifying strains by selecting rotten tremella fungus, expanding and culturing the strains, and inoculating the strains into sterilized Fermentation is carried out in fresh white fungus, and finally the fermented white fungus is dried, gelatinized, acid-treated, high-temperature treated, crushed and sieved to obtain the required white fungus oligosaccharides. The beneficial effects of the present invention are: by selecting rotten white fungus to cultivate and purify bacterial strains, the production environment requirements and production costs for the existence of the bacteria are reduced, biological, chemical and physical methods are used to degrade the white fungus polysaccharides, and efficient and controllable degradation of the white fungus polysaccharides is realized, It reduces the difficulty of industrialization and is suitable for industrial application.

Description

technical field [0001] The invention relates to the field of saccharide compound processing, in particular to a production process of tremella oligosaccharides. Background technique [0002] Oligosaccharides refer to straight-chain or branched low-polymerized sugar substances composed of 2-20 identical or different monosaccharides, also known as oligosaccharides, including two categories: ordinary oligosaccharides and functional oligosaccharides. Common oligosaccharides include sucrose, maltose, and lactose, which are familiar to people. These sugars can be digested and absorbed by the body, and have no growth-promoting effect on intestinal beneficial bacteria. Xylose, soybean oligosaccharides, chitosan oligosaccharides, tremella oligosaccharides, algin oligosaccharides, etc., cannot be digested and absorbed by the body, but have a variety of physiological activities, and have broad market space in the fields of food, agriculture, and medicine. . [0003] Tremella is a kin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12P19/00C12P19/12C08B37/00C07H3/06C07H1/00C07H1/06
CPCC12P19/04C12P19/00C12P19/12C08B37/0003C08B37/006C07H3/06C07H1/00C07H1/06
Inventor 赵守辉林志魁
Owner FUJIAN GLOBAL OCEAN BIOTECH
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