Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

In-vitro lung mechanical perfusate as well as preparation method and application thereof

A technology of perfusion liquid and lungs, applied in the field of isolated lung mechanical perfusion liquid and its preparation, can solve problems such as single source of Steen liquid, effective treatment of pneumonia, limited perfusion repair, etc., to improve the qualification rate of lung donors and reduce oxidative damage , the effect of reducing the preparation cost

Active Publication Date: 2021-01-29
WUXI PEOPLES HOSPITAL +1
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mechanical perfusion time of Steen solution on the isolated lung before transplantation is mostly about 4 hours. The lack of perfusion time limits the perfusion repair of the isolated donor lung by EVLP technology. Some diseases with relatively long treatment time, such as pneumonia caused by infection, are difficult to Get effective treatment during perfusion
The source of Steen fluid is single and must rely on imports. Its price is very high, and the price of perfusion fluid will bring an unbearable economic burden to patients

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In-vitro lung mechanical perfusate as well as preparation method and application thereof
  • In-vitro lung mechanical perfusate as well as preparation method and application thereof
  • In-vitro lung mechanical perfusate as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0049] One embodiment of the present invention also provides a method for preparing the above-mentioned isolated lung mechanical perfusate, including the following steps S10-S20.

[0050] Step S10: Provide raw materials according to the above-mentioned components of the mechanical perfusate of the isolated lung.

[0051] Step S20: Mix dextran, albumin, glutamine, amino acid, sorbitol, glucose and inorganic salts with sterile injection water.

[0052] In some of these embodiments, dextran, glutamine, amino acids, sorbitol, glucose and inorganic salts are dissolved in sterile injection water respectively, or multiple components are mixed and dissolved, albumin is dissolved in physiological saline for injection, filtered, Configure different solutions, and mix the different solutions configured.

[0053] In some preferred embodiments, step S20 includes the following steps S21-S29:

[0054] Step S21: Dissolving dextran and glucose with sterile injection water, and filtering at 0...

Embodiment 1

[0076] (1) Preparation of mechanical perfusion fluid for isolated lungs

[0077] 1. Dextran-40 glucose solution: Take 30g of dextran with a molecular weight of about 40kDa and 25g of anhydrous glucose, dissolve it in sterile water for injection to 500mL, and filter it at 0.22μm to obtain it;

[0078] 2. Human serum albumin solution: Take 80g of human serum albumin with a molecular weight of about 66kDa, dissolve it in sterile water for injection to 400mL, and filter it at 0.22μm to obtain it;

[0079] 3. Sodium chloride solution: Take 9g of sodium chloride, dissolve it in sterile water for injection to 1000mL, and filter it at 0.22μm to get it;

[0080] 4. Calcium chloride solution: take 0.5 g of anhydrous calcium chloride, dissolve it in sterile water for injection to 10 mL, and filter at 0.22 μm to obtain the solution;

[0081] 5. Composite potassium and magnesium aspartate solution: take 0.452 g of anhydrous potassium aspartate and 0.4 g of anhydrous magnesium aspartate, d...

Embodiment 2

[0106] The difference between this example and Example 1 lies in the different concentrations of the components contained in the isolated lung mechanical perfusion fluid, as shown in Table 3. Other steps and process parameters are basically the same as those in Example 1.

[0107] table 3

[0108] components Concentration g / L Human albumin solution 75 Dextran-40 2.4 glucose solution 2.0 glutamine solution 0.2 amino acid 0.2 Sorbitol 0.3

[0109] The isolated lung mechanical perfusion solution prepared in Example 2 was used for lung isolated perfusion repair, which can be maintained for 11 hours, and no new edema, hematoma or emphysema appeared in the lung.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to in-vitro lung mechanical perfusate as well as a preparation method and application thereof. The in-vitro lung mechanical perfusate comprises dextran, mammalian albumin, inorganic salt, glutamine, amino acid, sorbitol and glucose. The in-vitro lung mechanical perfusate can improve the energy metabolism of the in-vitro lungs, maintains a good gas exchange function within 12hours, and does not cause damage to the in-vitro lungs. Besides, the in-vitro lung mechanical perfusate can obviously reduce the consumption of the in-vitro lung mechanical perfusate in the actual useprocess, is matched with electrolyte replacement liquid for use, only consumes 2L of the in-vitro lung mechanical perfusate in the 12h EVLP process, effectively prolongs the EVLP implementation timeon the basis of cost reduction, and prolongs the preservation time of the in-vitro lungs.

Description

technical field [0001] The invention relates to the technical field of organ preservation, in particular to a mechanical perfusion solution for isolated lungs and its preparation method and application. Background technique [0002] With the continuous maturity of lung transplantation technology, lung transplantation has become the standard treatment for end-stage lung diseases that cannot be cured by drugs or surgery. However, the number of patients on the waiting list for lung transplantation is increasing year by year, and the shortage of transplantable lungs is becoming more and more serious. The utilization rate of donor lungs is only about 5%, which can only meet the lung transplantation needs of a very small number of people. [0003] The reason for the shortage of qualified donor lungs is that most of the donor lungs provided to the transplant team suffered from lung injury or related pulmonary complications before the death of the donor, and were not completely suit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0247
Inventor 陈静瑜卢艳卫栋林祥华刘峰刘强钟嘉伟杨振坤张勃毛禹康周鹏林冠金
Owner WUXI PEOPLES HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com