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A method for high production of short-chain fatty acids using microbial symbiotic fermentation technology

A short-chain fatty acid, symbiotic fermentation technology, applied in the field of microorganisms, can solve problems such as difficult application

Active Publication Date: 2022-03-22
山东省大健康精准医疗产业技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The short-chain fatty acid prepared by the above method is a chemical material, which is mainly used as an external carbon source for biological nitrogen and phosphorus removal to improve the efficiency of nitrogen and phosphorus removal, and is difficult to apply in the fields of food and medicine.

Method used

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  • A method for high production of short-chain fatty acids using microbial symbiotic fermentation technology

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Embodiment 1

[0033] A method for high-yielding short-chain fatty acids using microbial symbiotic fermentation technology, comprising the following steps:

[0034] 1) Jerusalem artichoke pretreatment: wash 50kg of fresh Jerusalem artichoke, after the water on the surface is naturally dried, put it into a beater for beating, beat at 20Hz for 10 minutes, and obtain Jerusalem artichoke pulp;

[0035] 2) Seaweed pretreatment: Wash 50kg of seaweed, and after the moisture on the surface dries naturally, cut it into 2-5cm long and 2-5cm wide blocks, and dry it in a blast drying oven (70°C) until the water content is 10%, placed in a grinder to grind to 60 mesh, and sieved to obtain seaweed powder;

[0036] 3) Preparation of fermented pulp medium: take 10 kg of Jerusalem artichoke pulp prepared in step 1), 2 kg of seaweed powder prepared in step 2), and 0.1 kg of sodium glutamate, disperse in 87.9 kg of water, mix, and homogenize under a pressure of 20 MPa for 8 minutes , adjust the pH to 6, keep it...

Embodiment 2

[0040] A method for high-yielding short-chain fatty acids using microbial symbiotic fermentation technology, comprising the following steps:

[0041] 1) Jerusalem artichoke pretreatment: wash 50kg of fresh Jerusalem artichoke, after the water on the surface is naturally dried, put it into a beater for beating, beat at 20Hz for 10 minutes, and obtain Jerusalem artichoke pulp;

[0042] 2) Seaweed pretreatment: Wash 50kg of seaweed, and after the moisture on the surface is naturally dried, cut it into 2-5cm long and 2-5cm wide blocks, and dry it in a blast drying oven (70°C) until the moisture content is 10%, placed in a grinder to grind to 60 mesh, and sieved to obtain seaweed powder;

[0043] 3) Preparation of fermented pulp medium: take 20 kg of Jerusalem artichoke pulp prepared in step 1), 5 kg of seaweed powder prepared in step 2), and 0.5 kg of sodium glutamate, disperse in 74.5 kg of water, mix, and homogenize under a pressure of 20 MPa for 8 minutes , adjust the pH to 7,...

Embodiment 3

[0047] A method for high-yielding short-chain fatty acids using microbial symbiotic fermentation technology, comprising the following steps:

[0048] 1) Jerusalem artichoke pretreatment: wash 50kg of fresh Jerusalem artichoke, after the water on the surface is naturally dried, put it into a beater for beating, beat at 20Hz for 10 minutes, and obtain Jerusalem artichoke pulp;

[0049] 2) Seaweed pretreatment: Wash 50kg of seaweed, and after the moisture on the surface is naturally dried, cut it into 2-5cm long and 2-5cm wide blocks, and dry it in a blast drying oven (70°C) until the moisture content is 10%, placed in a grinder to grind to 60 mesh, and sieved to obtain seaweed powder;

[0050] 3) Preparation of fermented pulp culture medium: 40 kg of Jerusalem artichoke pulp prepared in step 1), 10 kg of seaweed powder prepared in step 2), and 1 kg of sodium glutamate were dispersed in 49 kg of water, mixed, homogenized under a pressure of 20 MPa for 8 min, and adjusted The pH ...

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Abstract

The invention relates to a method for high-yielding short-chain fatty acids by utilizing microbial symbiotic fermentation technology. The present invention utilizes microbial symbiotic fermentation technology to produce high-yield short-chain fatty acids, takes Jerusalem artichoke and seaweed with high yield and easy acquisition as raw materials, that is, uses food-grade materials as fermentation substrates, and utilizes multi-bacteria symbiotic fermentation technology to produce short-chain fatty acids. Compared with the process of preparing short-chain fatty acids by using carbohydrate composition and pure cellulose in the technology, it overcomes the disadvantage of high cost of preparing short-chain fatty acids, and is simple to operate, safe and non-toxic, high in yield, easy to scale production, and has the advantages of The method has good economic benefits and wide application range, and provides new raw materials and new methods for preparing short-chain fatty acids.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for high-yielding short-chain fatty acids by utilizing microbial symbiotic fermentation technology. Background technique [0002] Short-chain fatty acids (SCFAs), also known as volatile fatty acids (Volatilefatty acids, VFA), are organic carboxylic acids with 1 to 6 carbon atoms, and are carbohydrates (oligosaccharides, oligosaccharides, After non-starch polysaccharides, resistant starch, etc.) enter the large intestine, the end products produced by bacterial fermentation mainly include acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, and valeric acid, of which 90% to 95% The SCFAs are acetic acid, propionic acid, and butyric acid. [0003] Short-chain fatty acids are the fermentation products of intestinal microorganisms, and their effects on the health of the body have been widely studied: ①SCFAs can improve intestinal di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/6409C12P39/00C12N1/20C12N1/16C12R1/01C12R1/645
CPCC12P7/6409C12P39/00C12N1/20C12N1/16
Inventor 朱之炜苏真真高丽鹤李文靖
Owner 山东省大健康精准医疗产业技术研究院
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