A plant-derived component and its application in anti-skin photoaging, improving skin renewal and repairing ability, and then preparing related cosmetics
A plant source and seed technology, applied in cosmetics, cosmetic preparations, preparations for skin care, etc., can solve the problems of decreased skin renewal and repair ability, decreased proliferation activity of skin fibroblasts, etc., and can promote the proliferation of human skin fibroblasts. , Improve skin renewal and repair ability, and promote the effect of skin renewal
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Embodiment 1
[0031] Embodiment 1: Preparation of witch hazel polysaccharide IV
[0032] 1. Test materials
[0033] Witch hazel seeds: After the fruits of witch hazel are ripe in autumn, the fruit branches are collected and placed in the sun for 2 to 3 days. The shells are cracked, and the seeds are taken out, dried at 55°C, and set aside.
[0034] Both the extraction solvent and the separation medium are conventional solvents and media, and the separation medium is used after being pretreated by a conventional method.
[0035] 2. Methods and results
[0036] 1. Extraction and separation and purification
[0037] Extraction of total polysaccharides: crush the dried witch hazel seeds through a 40-mesh sieve, take 200 g of powder, add 2 L of ultrapure water, extract for 3 hours at a constant temperature in a water bath at 80° C., and filter. Concentrate the filtrate to 100mL under reduced pressure, add 4 times the volume of absolute ethanol after deproteinization by Sevage method, let stan...
Embodiment 2
[0044]Embodiment 2: Anti-skin photoaging test
[0045] 1. Experimental materials
[0046] Hamamelis polysaccharides IV and V were prepared according to the method in Example 1, and stored in a dry place at room temperature for future use.
[0047] Female SD rats were purchased from Qinglongshan Animal Breeding Farm, Jiangning District, Nanjing, weighing 180-200 g.
[0048] 2. Experimental method
[0049] 1. Solution preparation
[0050] Glycerin solution of hamamelis polysaccharide: weigh appropriate amount of hamamelis polysaccharides IV and Ⅴ prepared in Example 1, and dissolve them ultrasonically in glycerin at 45° C. to prepare a hamamelis polysaccharide glycerol solution with a concentration of 2 mg / mL.
[0051] 2. Modeling and administration
[0052] After the rats were fed adaptively for one week, the hair on the back of the rats was removed by rosin paraffin wax plucking 24 hours before modeling, with a range of 2cm×3cm. They were randomly divided into blank group...
Embodiment 3
[0082] Embodiment 3: Improve skin renewal repair ability test
[0083] 1. Test materials
[0084] The brand of human skin fibroblast HSF is ATCC, purchased from Shanghai Yaji Biotechnology Co., Ltd.
[0085] Fetal bovine serum and DMEM medium were purchased from Gibco, USA.
[0086] Hamamelis polysaccharides IV and V were prepared according to the method in Example 1, and stored in a dry place at room temperature for future use.
[0087] MTT and DMSO were purchased from Sigma, USA.
[0088] 2. Test method
[0089] 1. Cell culture
[0090] Human skin fibroblasts HSF were inoculated in DMEM medium (complete medium) containing 10% fetal bovine serum, at 37°C, 5% CO 2 , Cultivate under the condition of relative saturated humidity, subculture once every 2-3 days.
[0091] 2. Determination of cell proliferation activity
[0092] Human skin fibroblast HSF in the logarithmic growth phase was taken, digested, and resuspended with complete medium to make the cell concentration 5×...
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