Probiotics for preventing and treating female genital tract inflammation

A bacterial and vaginal technology, applied in the field of probiotics, can solve the problems of weak ability, inability to well inhibit the reproduction of vaginal pathogenic bacteria, and low adhesion ability

Active Publication Date: 2021-02-09
SHANGHAI SINE PHARMA LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Therefore, the technical problem to be solved in this application is to overcome the Lactobacillus lactic acid and H in the prior art. 2 o 2 Defects such as weak ability, low adhesion ability and inability to well inhibit the reproduction of vaginal pathogenic bacteria

Method used

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  • Probiotics for preventing and treating female genital tract inflammation
  • Probiotics for preventing and treating female genital tract inflammation
  • Probiotics for preventing and treating female genital tract inflammation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: the preparation of culture medium

[0050] 1. Lactobacillus selective medium (Rogosa SL Agar) preparation:

[0051] (1) Weigh 22.5g of Rogosa SL Agar medium powder into a 500mL Erlenmeyer flask, add 300mL of deionized water, stir fully with magnetic force, then heat and boil for 1min under rapid stirring, and seal the bottle mouth;

[0052] (2) Put the above-mentioned sealed Erlenmeyer flask into a biological safety cabinet, and irradiate it with ultraviolet rays for more than 20 minutes;

[0053] (3) When the temperature of the medium drops to 50-60°C, open the bottle, add 396 μL of glacial acetic acid, put it in a microwave oven and boil for 2-3 minutes, and irradiate with ultraviolet light for more than 20 minutes;

[0054] (4) After the temperature of the culture medium has dropped to room temperature, pour it into a petri dish. Pour about 10-20mL of culture medium into each dish according to the size of the petri dish. After cooling and solidifying, ...

Embodiment 2

[0066] Example 2: Isolation, purification and enrichment culture of Lactobacillus crispatus, Lactobacillus gasseri and Lactobacillus salivarius strains

[0067] 1. Isolation, purification and enrichment culture of Lactobacillus strains

[0068] Several healthy women of childbearing age without vaginal infection or any intestinal disease were recruited to participate in providing samples. The participants all passed the health examination of the physical examination center, and provided information about their age (21-30), menstrual cycle and Information on other health behaviors, etc. Starting 2 weeks before the sample collection, all participants avoided all types of formulas containing probiotics. The samples were collected using the Port.A-Cd system of BD Company in the United States. Two sterile cotton swabs were used to collect the vaginal side wall of the subjects 1 / 3 of the secretion, put it into a sterile tube, and quickly transport it to the laboratory biosafety cab...

Embodiment 3

[0082] Example 3: Determination of metabolites of Lactobacillus crispatus, Lactobacillus gasseri and Lactobacillus salivarius

[0083] 1. Determination of lactic acid content in the fermentation liquid of Lactobacillus crispatus, Lactobacillus gasseri and Lactobacillus salivarius

[0084] The lactobacilli obtained in Example 2 were subjected to a preliminary test on stress resistance such as oxygen resistance and low temperature resistance, and 5 strains of Lactobacillus crispatus, 3 strains of Lactobacillus gasseri and 7 strains of Lactobacillus salivarius were obtained through screening. 5 strains of Lactobacillus crispatus, 3 strains of Lactobacillus gasseri, 7 strains of Lactobacillus salivarius and the control strain Lactobacillus delbrueckii DM8909 were detected by D-lactic acid detection kit (Sigma) and L-lactic acid detection kit (Sigma) , Lactobacillus rhamnosus PB01, Lactobacillus rhamnosus GR-1, ATCC standard strain 33820 (purchased from ATCC) were fermented in the ...

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Abstract

The invention relates to probiotics for preventing and treating female genital tract inflammation. The invention relates to lactobacillus for producing lactic acid and H2O2, a thallus lysis componentor a metabolite of the lactobacillus. The lactobacillus is selected from Lactobacillus crispatus, Lactobacillus gasseri or Lactobacillus salivarius, the preservation number of the Lactobacillus crispatus is CGMCC No. 19239, the preservation number of the Lactobacillus gasseri is CGMCC No. 19238, and the preservation number of the Lactobacillus salivarius is CGMCC No. 19236.

Description

technical field [0001] The invention relates to probiotics for preventing and treating female reproductive tract inflammation. The probiotics can regulate vaginal microenvironment and inhibit the growth of vaginal pathogenic bacteria, thus preventing and assisting treatment of female reproductive tract inflammation. Background technique [0002] There are a variety of microorganisms in the vagina of healthy women, and they form a vaginal micro-ecological system that restricts, coordinates and dynamically balances with the host and the environment. It is reported in the literature that the vaginal flora of healthy women is mainly composed of Lactobacillus, and a certain type of Lactobacillus is absolutely dominant. Under normal circumstances, Lactobacillus can protect the vagina, but the disturbance of vaginal microecology dominated by Lactobacillus can lead to vaginitis. [0003] The occurrence of bacterial vaginosis (BV) is due to the imbalance of vaginal flora and the red...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P15/02A61P31/12A61P31/10A61P31/04A61P13/02A61P15/00C12R1/225
CPCA61K35/747A61P15/02A61P31/12A61P31/10A61P31/04A61P13/02A61P15/00C12R2001/225C12N1/205
Inventor 徐晓芬于鸿晶王莎莎梁席孙宁云
Owner SHANGHAI SINE PHARMA LAB
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