Nostoc sphaeroids kutz polyculture method

A technology of Gexianmi and Gexianmi algae, applied in the direction of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem of small number of mixed cultured microalgae, great influence of external environmental factors, and problems in cultivating Low density and other problems, to achieve the effect of preventing bacterial contamination, increasing diversity, and rich nutrition

Inactive Publication Date: 2021-02-09
CHANGDE YANDI BIOTECH LTD CO +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of photosynthetic autotrophy are low culture density, long culture period, slow growth, open culture is greatly affected by external environmental factors, and it is easy to pollute miscellaneous algae and microorganisms. Therefore, it needs to consume a lot of manpower for physical cleaning and screening. Microbial risk, sterilization required
In recent years, the application of polyculture technology in other microalgae (such as chlorella, euglena, Scenedesmus, etc.) has achieved remarkable results. report

Method used

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  • Nostoc sphaeroids kutz polyculture method
  • Nostoc sphaeroids kutz polyculture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0062] Embodiment 3: Detection of protein and crude polysaccharide content

[0063] The protein content is determined according to the Kjeldahl method in GB5009.5-2016

[0064] Crude polysaccharide content was determined according to the sulfuric acid-phenol method in NYT1676-2008

Embodiment 4

[0065] Example 4: Dry matter yield and wet weight growth rate

[0066] Dry product yield (R), select 150g of fresh kudzu rice, absorb the surface moisture with absorbent paper, weigh 100g, dry at 55°C for 48 hours, then collect the dry product and weigh it to get the dry product yield .

[0067] Wet weight growth rate: record the initial inoculum size (m) and at the end of the cultivation, use a sampling sieve to collect all Ge Xianmi, drain in the sampling sieve for 15 minutes, weigh the total weight (M), and calculate according to the following formula, wet weight rate of growth.

[0068]

[0069] S: growth rate of wet weight (g / 100g.day); M: total weight of fresh Pueraria japonica at harvest (g or kg);

[0070] m: total initial inoculation weight of Gexianmi (g or kg); T: culture period (day).

[0071] Table 1 Test results

[0072]

[0073] The results in Table 1 show that polyculture can accelerate the average daily growth rate of Gexianmi, the protein content an...

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Abstract

The invention relates to a nostoc sphaeroids kutz polyculture method. The method comprises the steps of nostoc sphaeroids kutz algae species polyculture domestication, polyculture medium preparation,inoculation, fermentation tank polyculture and the like. Review bioactive water adopted by the method disclosed by the invention is brand-new agricultural water, has rich nutrition and antibacterial and bactericidal effects, can provide nutrition for nostoc sphaeroids kutz in polyculture, and can also play a role in preventing infectious microbe pollution. EOW (acidic electrolyzed oxidizing water)is used for spectral sterilization, is non-toxic and harmless to nostoc sphaeroids kutz, has little harm to the nostoc sphaeroids kutz, effectively protects the activity of nostoc sphaeroids kutz algae cells, is beneficial to human bodies, and does not bring food safety risks. The nostoc sphaeroids kutz algae species with strong polyculture adaptability are obtained by combining ultraviolet raysand an organic carbon source for domestication culture. A dissolved oxygen index is used as an alarm signal, so that light and dark alternate culture regulation and control are effectively realized, and the poison of the dissolved oxygen to the polyculture nostoc sphaeroides is effectively avoided. A heterotrophic carbon source is hydrolytic enzymolysis starch, so that the diversity of heterotrophy to the existence form of the carbon source is increased. In the polyculture mode, the nostoc sphaeroids kutz grows in a photoautotrophic mode, and grows in a heterotrophy mode, the whole heterotrophy process is in a relatively sterile state, and the influence by the outside is small.

Description

technical field [0001] The invention relates to the technical field of cultivation of Radix Radix et Rhizoma, in particular to a method for polyculture of Radix Radix et Rhizoma. Background technique [0002] Kudzu rice is the common name for Nostoc sphaeoides Kützing in the genus Nostoc Vauch, which belongs to Cyanophyta, Cyanophyceae, Nostocales, Nostocaceae . Ge Xian Mi, also known as green bird's nest, is very precious and is a good product for both food and medicine. The total protein content of the dry matter is 28.38% to 52%, and the total sugar is 24.19%. Contains 18 amino acids, including 8 essential amino acids for the human body; rich in V C , V E And B vitamins, and a variety of mineral elements, especially edible algae with high calcium content. [0003] There are three ways to cultivate microalgae: autotrophy, heterotrophy, and polyculture. At present, the culture of kudzu is mainly used in suspension culture, running track tank stirring culture, static g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/20C12R1/01
CPCC12N1/36C12N1/20
Inventor 王卫易思富周丰田云文戈郭小玲彭锦桃侯丽华杨胜辉
Owner CHANGDE YANDI BIOTECH LTD CO
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