Preparation method of self-powered bipolar micro-electrode micro-fluidic chip photoelectrochemical aptamer sensor
A technology of aptamer sensor and microfluidic chip, which is applied in chemical instruments and methods, electrochemical variables of materials, scientific instruments, etc., can solve the problems of cumbersome detection methods, high cost, high detection limit, etc., and achieve high repeatability , low detection limit and high detection sensitivity
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Embodiment 1
[0033] A method for preparing a self-powered bipolar microelectrode microfluidic chip photoelectrochemical aptasensor, the preparation steps are as follows:
[0034] (1) Design and draw microfluidic channel graphics with computer design software AUTOCAD;
[0035] (2) Use the designed pattern to draw the mask, and process the microfluidic polydimethylsiloxane PDMS chip with standard soft lithography technology;
[0036] (3) The 5 cm × 5 cm ITO conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water for 30 min, respectively, and dried with nitrogen, and the cleaned ITO conductive glass was etched to obtain the micro working electrode 1 and the bottom plate of the micro reference / counter electrode 2;
[0037] (4) 20 µL, 5.0 mg / mL iodine-doped bismuth oxychloride I-BiOCI solution was drop-coated on the micro-working electrode, dried at room temperature, and 10 µL, 0.1% (w / v) chitosan was added dropwise The solution contained 1% acetic acid on the su...
Embodiment 2
[0044] A method for preparing a self-powered bipolar microelectrode microfluidic chip photoelectrochemical aptasensor, the preparation steps are as follows:
[0045] (1) Design and draw microfluidic channel graphics with computer design software AUTOCAD;
[0046] (2) Use the designed pattern to draw the mask, and process the microfluidic polydimethylsiloxane PDMS chip with standard soft lithography technology;
[0047] (3) The 5 cm × 5 cm ITO conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water for 30 min, respectively, and dried with nitrogen, and the cleaned ITO conductive glass was etched to obtain the micro working electrode 1 and the bottom plate of the micro reference / counter electrode 2;
[0048] (4) 20 μL, 6.0 mg / mL iodine-doped bismuth oxychloride I-BiOCI solution was drop-coated on the micro-working electrode, dried at room temperature, and 10 μL, 0.1% (w / v) chitosan was added dropwise The solution contained 1% acetic acid on the su...
Embodiment 3
[0055] A method for preparing a self-powered bipolar microelectrode microfluidic chip photoelectrochemical aptasensor, the preparation steps are as follows:
[0056] (1) Design and draw microfluidic channel graphics with computer design software AUTOCAD;
[0057] (2) Use the designed pattern to draw the mask, and process the microfluidic polydimethylsiloxane PDMS chip with standard soft lithography technology;
[0058] (3) The 5 cm × 5 cm ITO conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water for 30 min, respectively, and dried with nitrogen, and the cleaned ITO conductive glass was etched to obtain the micro working electrode 1 and the bottom plate of the micro reference / counter electrode 2;
[0059] (4) 20 μL, 7.0 mg / mL iodine-doped bismuth oxychloride I-BiOCI solution was drop-coated on the micro-working electrode, dried at room temperature, and 10 μL, 0.1% (w / v) chitosan was added dropwise The solution contained 1% acetic acid on the su...
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