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Viola mandshurica extract, method for extracting same, and use thereof

A viola extract and technology, applied in application, food science, drug combination, etc., can solve the problems of accelerating skin aging, reducing the total number of cells, and not being able to effectively protect the inner layer of skin

Inactive Publication Date: 2021-02-23
百岳特生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If fibroblasts are affected by other external environmental damage such as ultraviolet rays, reducing their proliferation rate or even reducing the total number of cells, it will cause damage and thinning of the skin surface, which will not be able to effectively protect the inner layer of skin, thereby accelerating the process of skin aging. overall aging rate

Method used

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  • Viola mandshurica extract, method for extracting same, and use thereof
  • Viola mandshurica extract, method for extracting same, and use thereof
  • Viola mandshurica extract, method for extracting same, and use thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0051] [Example 1] Preparation of Zihua Diding Juice

[0052] In water (extraction solvent), add its total weight 0.1wt% pectinase (available from Novozymes, model Novozymes L), and then add the powder ground from the whole plant of Viola chinensis (origin: China) into the water containing pectinase (wherein the weight ratio of water: Viola chinensis powder is 30:1), make it mix Then raise the temperature of this liquid to and maintain at 55±5°C, and keep soaking for about 60 minutes to carry out the first stage of extraction. Then, the temperature of the liquid was raised to and maintained at 85±5° C. for about 30 minutes to carry out the second-stage extraction.

[0053] After the heating was stopped, the extraction solvent was filtered through a 400-mesh sieve to remove solids to obtain a supernatant. Concentrate the supernatant under reduced pressure at 60±5°C with a concentrator (purchased from BUCHI, model Rotavapor R-100) until the Brix value (Degrees Brix) of the so...

example 2

[0054] [Example 2] Total flavonoid content test

[0055] After diluting the viola extract obtained in Example 1 with water 10 times according to its volume, take 200 μL into a test tube. Add 200 μL of 5wt% sodium nitrite (purchased from Sigma, product number 31443) aqueous solution into this test tube, mix well and let stand for reaction for 6 minutes. Then add 200 μL of 10wt% aluminum nitrate (purchased from Alfa Aesar, product number 12360) aqueous solution, mix well and let stand for reaction for 6 minutes, then add 2 mL of 4wt% sodium hydroxide aqueous solution (sodium hydroxide is purchased from Macron, product number 7708-10 ) to make it evenly mixed. Finally, add 1.4 mL of water to the test tube and mix evenly to obtain the reaction solution. Take 200 μL of the reaction solution in the test tube to a 96-well reaction plate, and use a spectrophotometer (Jasco, model V-730) to detect the absorbance at a wavelength of 500 nm. .

[0056] Standards are then prepared to cr...

example 3

[0059] [Example 3] Total polyphenol content test

[0060] After diluting the viola extract obtained in Example 1 with water 10 times according to its volume, take 100 μL into a test tube. Then, add 500 μL of Folin-Ciocalteu's phenol reagent (Folin-Ciocalteu's phenol reagent, purchased from Merck, product number 1.09001.0100) to this test tube, mix well and let stand for 3 minutes, then add 400 μL of 7.5wt% sodium carbonate (purchased from Sigma, commodity number 31432) aqueous solution in test tube, make it mix uniformly and react for 30 minutes. After ensuring no air bubbles in the solution by vortex, 200 μL of the solution was taken to measure the absorbance at a wavelength of 750 nm.

[0061] Next, prepare a standard to create a calibration curve. In this case, gallic acid (Gallic acid) equivalent is used as the expression of the relative content of total polyphenols. Here, prepare 0 μL / mL, 20 μL / mL, 40 μL / mL, 60 μL / mL, 80 μL / mL, and 100 μL / mL standard solutions of galli...

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Abstract

The invention provides application of a viola mandshurica extract in preparation of a composition for promoting fibroblast growth, application in preparation of a composition for improving granulocyteactivity and application in preparation of a composition for improving skin gloss.

Description

technical field [0001] The present invention provides a use of viola extract, in particular to the use of viola extract for preparing a composition for improving skin condition. Background technique [0002] The skin is the largest barrier to protect the human individual, and it has the function of resisting water loss, pathogenic bacteria and various external environmental damage. When the skin is exposed to a large amount of ultraviolet rays (ultraviolet, UV), ionizing radiation, drugs or xenobiotics, it will promote the generation of reactive oxygen species (reactive oxygen species, ROS) in the skin. When the concentration of the accumulated reactive oxygen species exceeds the antioxidant capacity of the cell or tissue itself, oxidative stress will be formed, and the reactive oxygen species will interact with the components in the cell (including DNA, protein, lipid, etc. ) to react. When skin cells are damaged, it will have undesired effects on the skin and even accele...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61Q19/08
CPCA61P17/00A61Q19/00A23V2002/00A23L33/105A61K36/898A61K8/9794A61P39/00A23V2200/318A23V2250/21
Inventor 林咏翔姚采涵
Owner 百岳特生物科技(上海)有限公司
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