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A kind of method that biomass chemical-enzymatic method prepares L-furanserine

A technology of furanserine and glycine, which is applied in chemical instruments and methods, catalyst activation/preparation, physical/chemical process catalysts, etc., to achieve good biocompatibility, simple steps, economical and environmentally friendly processes

Active Publication Date: 2022-02-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of traditional organic synthesis of L-furanserine, the present invention proposes an efficient one-pot chemical-enzyme catalysis process approach to directly convert lignocellulosic raw materials into L-furanserine

Method used

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  • A kind of method that biomass chemical-enzymatic method prepares L-furanserine
  • A kind of method that biomass chemical-enzymatic method prepares L-furanserine
  • A kind of method that biomass chemical-enzymatic method prepares L-furanserine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Solid Acid Fe 3 O 4 @ MCM-41 / SO 4 2– Preparation

[0040] Magnetic solid acid Fe 3 O 4 @ MCM-41 / SO 4 2– Preparation as follows:

[0041] (1) Put FECL 3 · 6h 2 O and FECL 2 · 4h 2 O Dissolve in the stirred reactor, where Fe 3+ FE 2+ The molar ratio is 2.0: 1.1. Under nitrogen protection, heated to 85 ° C, stirring with NH at a high speed of 1000R / min. 3 · H 2 O Solution to the reaction liquid pH of 11, separating the deposited product by the magnetic field, ie gave Fe 3 O 4 Magnetic particles.

[0042] (2) mixing the fly ash material and mass fraction of 20% hydrochloric acid in mass ratio of 1:10, stirred at 80 ° C for 4 h, remove impurities such as iron, calcium, then filtered, washed, dried to obtain pretreatment powder coal ash. The sodium hydroxide is then uniformly mixed with the pre-treated fly as average of 1: 2, and heated at 550 ° C for 1 h. The resulting alkali flour coal ash was mixed with deionized water at a ratio of 1: 4, stirred at room temp...

Embodiment 2

[0046] Example 2 Solid acid Fe 3 O 4 @ MCM-41 / SO 4 2– Catalytic corn core prepared

[0047] 100 mL of deionized water, 10 g of corn core, and final concentration 22 g / l, for the magnetic solid acid Fe 3 O 4 @ MCM-41 / SO 4 2– The high pressure reactor was rapidly heated to 180 ° C and stirred for 40 min by electrical heating sleeve. After the reaction, the reactor was immediately soaked in an ice water bath to cool to room temperature.

[0048] After testing, the concentration of furfural after the reaction was 72 mm.

Embodiment 3

[0049] Example 3 Biomass furfural a pot method synthesized L-furan alkine

[0050] The recombinant L-threonine aldolase whole cells were prepared as follows: the engineering of L-threonine aldolase genes (the construction method of engineering bacteria) is as follows: gong, l., et al.applied Biochemistry andbiotechnology , 2020.DOI: ORG / 10.1007 / S12010-020-03447-Y) vaccinated to 50 μg · ml –1 The lb liquid medium of kanamycin was cultured at 37 ° C overnight. Then inoculate the amount of inoculated by 1% (volume concentration) to 50 μg · ml –1 In LB liquid medium of kanamycin, 37 ° C, 180 rpm culture to a bacterial concentration OD 600 In 0.6-1.0, IPTG of 0.2 mm was added, 25 ° C induced 10 h, 4 ° C, 8000 rpm centrifuge 10 min to collect wet bacteria, that is, recombinant L-threonine aldolase whole cells, and enzyme activity assay The whole cell enzyme is affected by 4000-80000U / g.

[0051] 100 ml of deionized water, 10g corn coincinal powder, 2 wt% magnetic solid acid Fe 3 O...

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Abstract

The invention discloses a method for preparing L-furanserine by a biomass chemical-enzymatic method, which belongs to the field of biochemical industry. The invention provides a Fe with optimized preparation process 3 o 4 @MCM‑41 / SO 4 2– , and use it in the process of biochemical-enzymatic production of L-furanserine. The production method of L-furanserine of the present invention saves the separation process of intermediates, has good biocompatibility, simple steps, economical and environment-friendly process, and can make the yield of L-furanserine reach 73.6%, which is β-hydroxy- Green and sustainable manufacturing of α‑amino acids provides a reference.

Description

Technical field [0001] The present invention relates to a method for preparing L-furanase-enzymatic method for biomass chemistry, which belongs to the field of biochemical. Background technique [0002] Biomass utilization can not only relieve fossil energy depletion and environmental pollution crisis, but also increase the use of waste resources, and supports agricultural economic development. [0003] Furfural, a furan formaldehyde, is a furan-based derivative as a bio-based platform compound, can be further converted to fine chemicals and fuels. One-step dilute furfural production is less in investment in its equipment, and it has been widely used in the furfural industry. However, a traditional step-by-step dilute acid production process is low, high energy consumption, severe pollution, and this process only uses hemicellulose in biomass, and cellulose is not utilized, and the raw material utilization rate is low. In order to promote the further development of the furfural i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J29/03B01J37/10C12P17/04C07D307/48
CPCB01J29/0333B01J37/10C12P17/04C07D307/48B01J2229/60B01J2229/20B01J35/33
Inventor 倪晔龚磊修元松董晋军许国超韩瑞枝
Owner JIANGNAN UNIV
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