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High-yield bacillus mucilaginosus Industrial production fermentation process

A colloidal bacillus and fermentation process technology, applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of high cost, low product quality, and inability to produce high yields, so as to reduce fermentation costs, increase the number of viable fermentation bacteria, and increase profit value Effect

Pending Publication Date: 2021-02-26
朝阳华星生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the production process of the existing colloidal bacillus, due to the high cost of raw materials in the culture medium during the cultivation and fermentation steps, mass production and fermentation cannot be realized, and the number of viable fermentation bacteria in the existing fermentation technology is low , resulting in low product quality and incapable of high yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A high-yield colloidal bacillus industrial production fermentation process, comprising: 1) primary culture: take a proper amount of aerosol bacillus and inoculate it into an eggplant bottle containing a culture solution for activation treatment, and cultivate it in a culture shaker to obtain primary seeds. Inoculate the primary seeds into a 50L seed tank, adjust the pH value before actual sterilization to 7.5, and cultivate at a temperature of 32°C for 15 hours in an aerobic environment, and put into the seed medium to form primary seeds;

[0023] 2) Secondary cultivation: inoculate primary seeds into a 300L seed tank, adjust the pH value before actual sterilization to 7.5, and cultivate at 32°C for 18 hours in an aerobic environment, and put in seed medium to form secondary seed;

[0024] 3) Tertiary cultivation: sterilize the 5000L seed tank at a temperature of 121°C for 30 minutes, adjust the pH value of the 5000L seed tank to 7.5, inoculate the secondary seeds into ...

Embodiment 2

[0029] A high-yield colloidal bacillus industrial production fermentation process, comprising: 1) primary culture: take a proper amount of aerosol bacillus and inoculate it into an eggplant bottle containing a culture solution for activation treatment, and cultivate it in a culture shaker to obtain primary seeds. Inoculate the primary seeds into a 50L seed tank, adjust the pH value before actual sterilization to 7.7, and cultivate at a temperature of 38°C for 19 hours in an aerobic environment, and put into the seed medium to form primary seeds;

[0030] 2) Secondary cultivation: Inoculate primary seeds into a 300L seed tank, adjust the pH value before actual sterilization to 7.7, and cultivate at 38°C for 21 hours in an aerobic environment, and put in seed medium to form secondary seed;

[0031] 3) Tertiary cultivation: adjust the pH value of the 5000L seed tank to 7.7, carry out the actual sterilization treatment on the 5000L seed tank at a temperature of 124°C for 30 minute...

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PUM

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Abstract

The invention discloses a high-yield bacillus mucilaginosus industrial production fermentation process. The problems of low yield and high cost in the bacillus mucilaginosus processing process are solved. According to the key point of the technical scheme, the high-yield bacillus mucilaginosus industrial production fermentation process comprises the steps of primary culture, secondary culture, tertiary culture, fermentation tank transfer and drying treatment, and is applied to a 5000L seed tank and a 60m<3> fermentation tank. The raw materials of a seed culture medium and a fermentation culture medium are the same, the seed culture medium and the fermentation culture medium are each prepared from the raw materials: soybean meal, cane sugar, yeast extract, dipotassium phosphate, magnesium sulfate and calcium carbonate, and the purposes of increasing the product yield and reducing the production cost are achieved.

Description

technical field [0001] The invention belongs to the technical field of bacillus processing, in particular to a high-yielding colloid bacillus industrial production fermentation process. Background technique [0002] In agriculture, Bacillus colloidis has the characteristics of dissolving potassium and phosphorus and is easy to form dominant species of root system. It is a relatively important kind of microbial fertilizer products in my country at present. In terms of industry, Bacillus glialis is easy to produce significant extracellular polysaccharide capsules in nitrogen-free medium or larger C / N medium. The use of this polysaccharide flocculation to produce microbial flocculants with obvious advantages has broad prospects. In the metallurgical industry, Bacillus colloidus can be used in bacterial leaching and improving the properties of certain mineral materials, especially for the development of low-grade minerals and the recovery and utilization of some useful metals. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N3/00C12R1/07
CPCC12N1/20C12N3/00
Inventor 韩国宝梁爱民张天航卢倩雯
Owner 朝阳华星生物工程有限公司
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