Process for producing arginine by fed-batch culture solution fermentation
A technology of fed culture and arginine, applied in the field of microbial fermentation, can solve the problems of low acid production capacity and conversion rate, immature technical conditions of arginine, and high cost of arginine, so as to avoid uncontrolled fermentation and cost. Increase, improve synthesis efficiency, reduce the effect of metabolic by-product accumulation
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Embodiment 1
[0035] A process for producing arginine by fed-batch culture broth fermentation, comprising:
[0036] Step 1) The activated arginine-producing strain Escherichia coli CGMCC No.11674 bacterial liquid (1×10 8 cfu / ml) into the seed tank for 24 hours of seed culture to obtain the seed liquid, the culture temperature is 38°C, the pH is 6.8, the air volume is 200L / h, the rotation speed is 350rpm, and the tank pressure is 0.04mpa.
[0037] Step 2) Inoculate the seed liquid with 12% inoculum amount in a 100L fermenter with 50L fermentation medium for cultivation, the rotation speed is 280rpm, the temperature is 34°C, and the pH is 7.1 (pH value is controlled by adjusting the ammonia water). The pressure is 0.03mpa, the dissolved oxygen is controlled at more than 18% by alternately increasing the rotation speed and air volume, and the fermentation cycle is 55h.
[0038] The process of adding culture medium during fermentation is as follows:
[0039] 1) When the fermentation culture r...
Embodiment 2
[0046] A process for producing arginine by fed-batch culture broth fermentation, comprising:
[0047] Step 1) The activated arginine-producing strain Escherichia coli CGMCC No.11674 bacterial liquid (1×10 8 cfu / ml) into the seed tank for seed culture for 24 hours to obtain the seed liquid, the culture temperature is 38°C, the pH is 6.9, the air volume is 200L / h, the rotation speed is 300rpm, and the tank pressure is 0.05mpa.
[0048] Step 2) Inoculate the seed liquid with 12% inoculum amount in a 100L fermenter with 50L fermentation medium for cultivation, the rotation speed is 300rpm, the temperature is 34°C, and the pH is 7.2 (pH value is controlled by adjusting the ammonia water). The pressure is 0.04mpa, the dissolved oxygen is controlled at more than 18% by alternately increasing the rotation speed and air volume, and the fermentation cycle is 55h.
[0049] The process of adding culture medium during fermentation is as follows:
[0050] 1) When the fermentation culture re...
Embodiment 3
[0068] Effect of fermentation medium components on arginine production.
[0069] 1. On the basis of Comparative Document 1, additionally add different carbon sources, including sucrose and glycerol, the added concentrations are 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and the unit is g / L, such as figure 1 As shown, the addition of sucrose has no obvious effect on the production of arginine, but glycerol can increase the production of arginine. With the increase of glycerol concentration, the content of arginine increases, but when the concentration increases to 5g / L, it is close to When the peak value continued to increase, the production of arginine did not change significantly. Considering factors such as yield and cost comprehensively, when the concentration of glycerin is 5g / L, it is the optimal amount to add.
[0070] 2. To verify the effect of the amount and composition of glucose aqueous solution on the acid production of arginine.
[0071] Experiments were conducted to ve...
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