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Kit for determining SAA based on latex enhanced immunoturbidimetry, and preparation method of kit

An immunoturbidimetric and latex-enhanced technology, applied in biological testing, measuring devices, instruments, etc., can solve the problems of small linear range and low sensitivity of detecting SAA, and achieve the effect of wide linear range, fast detection speed and easy operation

Pending Publication Date: 2021-03-02
ANHUI DAQIAN BIO ENG LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a test kit and a preparation method for determining SAA based on latex-enhanced immunoturbidimetric method, aiming to solve the shortcomings of low sensitivity and small linear range of detection SAA circulating in the existing market, and fully automatic biochemical analysis can be used The instrument detects SAA, which is easy to operate, fast in detection speed, high in precision and good in repeatability

Method used

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  • Kit for determining SAA based on latex enhanced immunoturbidimetry, and preparation method of kit
  • Kit for determining SAA based on latex enhanced immunoturbidimetry, and preparation method of kit
  • Kit for determining SAA based on latex enhanced immunoturbidimetry, and preparation method of kit

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The test kit based on the latex-enhanced immune turbidimetric method for measuring SAA comprises reaction buffer and latex reagent, and the reaction buffer comprises 0.6g / L glycine buffer solution, 0.9g / L magnesium chloride, 10g / L magnesium chloride by weight. Polyethylene glycol 1000, 0.5ml / L of Proclin300 and 15g / L of BSA, the solvent is purified water, and the latex reagent includes at least two kinds of latex microspheres coupled by monoclonal antibodies.

[0027] Further, the volume ratio of the reaction buffer to the latex reagent is 3:1.

[0028] Further, the particle size of the latex microspheres is 90nm.

[0029] The preparation method of the kit for measuring SAA based on latex-enhanced immunoturbidimetric method comprises the following steps:

[0030] Step 1, measure 0.2ml of latex microspheres with a solid content of 10%, add to 3ml of phosphate buffer, then centrifuge at a speed of 20,000 rpm, remove the supernatant, and use 3ml of MES buffer for the rema...

Embodiment 2

[0037] The test kit based on the latex-enhanced immunoturbidimetric method for measuring SAA includes reaction buffer and latex reagent, and the reaction buffer includes 2.56g / L tris buffer solution and 15.4g / L chlorine in parts by weight Proclin300 and the sucrose of the Proclin300 and 36g / L of sodium chloride, 25g / L of 25g / L, the sucrose of 36g / L, the solvent is purified water, and described latex reagent comprises at least two kinds by monoclonal antibody and latex microsphere coupling become.

[0038] Further, the volume ratio of the reaction buffer to the latex reagent is 5:1.

[0039] Further, the particle diameter of the latex microspheres is 100nm.

[0040] The preparation method of the kit for measuring SAA based on latex-enhanced immunoturbidimetric method comprises the following steps:

[0041] Step 1, measure 0.6ml of latex microspheres with a solid content of 10%, add to 4ml of phosphate buffer, then centrifuge at a speed of 20,000 rpm, remove the supernatant, a...

Embodiment 3

[0048] The test kit for measuring SAA based on latex-enhanced immunoturbidimetric method includes reaction buffer and latex reagent, and the reaction buffer includes 4g / L 2-(N-morpholine)ethanesulfonic acid (MES) buffer in parts by weight , the zinc chloride of 30g / L, the macrogol 6000 of 40g / L, the sucrose of the thimerosal of 2ml / L and 60g / L, solvent is purified water, and described latex reagent comprises at least two kinds by monoclonal antibody and latex Microspheres are coupled.

[0049] Further, the volume ratio of the reaction buffer to the latex reagent is 4:1.

[0050] Further, the particle size of the latex microspheres is 300nm.

[0051] The preparation method of the kit for measuring SAA based on latex-enhanced immunoturbidimetric method comprises the following steps:

[0052] Step 1, measure 1ml of latex microspheres with a solid content of 10%, add to 5ml of phosphate buffer, then centrifuge at a speed of 20,000 rpm, remove the supernatant, and use 5ml of MES ...

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Abstract

The invention provides a kit for determining SAA based on latex enhanced immunoturbidimetry, and a preparation method thereof. The kit comprises a reaction buffer solution and a latex reagent, the reaction buffer solution comprises, by weight, 0.6-4 g / L of a buffer solution, 0.9-30 g / L of an inorganic salt, 10-40 g / L of a turbidity enhancer, 0.5-2 ml / L of a preservative and 15-60 g / L of a stabilizer, the solvent is purified water, and the latex reagent is formed by coupling at least two kinds of latex microspheres through monoclonal antibodies. A full-automatic biochemical analyzer can be usedfor detecting the SAA, operation is easy and convenient, the detection speed is high, precision is high, repeatability is good, detection sensitivity and specificity can be improved at the same time,the linear range of the kit is wider, the accuracy is higher, and clinical requirements are better met.

Description

technical field [0001] The invention relates to the field of preparation of biological detection reagents, in particular to a kit for determining SAA based on latex-enhanced immune turbidimetry and a preparation method thereof. Background technique [0002] Serum amyloid protein A (serum amyloid protein A, SAA) has multiple isomers, which are secreted by the liver, among which acute SAA (acute SAA, A-SAA) has been widely used in the auxiliary diagnosis of viral and bacterial infections. Under normal circumstances, the content of SAA in human plasma in the acute phase is very small, and when the body is in the acute phase reaction, it begins to increase rapidly in about 5 to 6 hours. [0003] In the liver, SAA is mainly combined with high-density lipoprotein (high density lipoprotein, HDL) and transported to the outside of the liver through the blood. SAA has 4 isomers, SAA1, SAA2, SAA3 and SAA4, the latter two hardly enter the bloodstream after being synthesized by the live...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/543G01N33/531
CPCG01N33/68G01N33/577G01N33/54346G01N33/531G01N2333/47
Inventor 芮双印李祥宇
Owner ANHUI DAQIAN BIO ENG LIMITED