Organic lentinan soluble ball mixture capable of improving intestinal immunity and preparation method thereof
A technology of lentinan and immunity, applied in polysaccharide/gum-containing food ingredients, bacteria used in food preparation, dairy products, etc., to eliminate toxins in the body, avoid protein denaturation, and enhance the body's immune and disease resistance effects
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[0059] The preparation method of the lentinan soluble beans for improving intestinal immunity comprises the following steps:
[0060] 1) Weigh by weight: lentinan solution 10-40 parts, trehalose 5-10 parts, skimmed milk powder 100-120 parts, whey powder 10-20 parts, corn starch 25-30 parts, acetylated di 50-100 parts of starch phosphate, 1-5 parts of baking powder, 1-5 parts of compound probiotics, 0.5-1 part of multivitamins; wherein, the organic lentinan solution is prepared by the following steps:
[0061] a. Preparation of organic lentinan crude product:
[0062] Wash well-grown and non-mold organic fruiting bodies of mushrooms, cut off the roots, cut the caps into cubes, dry them at 70-80°C, grind them into powder, add cellulase and papain for enzymatic hydrolysis, and then add bile chloride Alkali-1,4-butanediol deep eutectic solvent extraction, filtration, the resulting filtrate was dialyzed to remove impurities, microfiltration activated carbon adsorption decolorizati...
Embodiment 1
[0103] The preparation method of the lentinan soluble beans for improving intestinal immunity comprises the following steps:
[0104] 1) Lentinan solution is prepared by the following steps:
[0105] a. Preparation of crude lentinan:
[0106] ①Wash well-grown and non-mold mushroom fruiting bodies, cut off the roots, cut the mushroom caps into cubes, dry them at a temperature of 100-120°C, and grind them into powder.
[0107] 2. Add cellulase (concentration of cellulase is 0.5wt%, aqueous solution) in powder, be that 45 ℃, pH are enzymolysis 45min under the condition of 4.6 at temperature; Then add papain (concentration of papain is 1.5wt%) , aqueous solution), at a temperature of 50°C and a pH of 6.1, enzymatic hydrolysis for 30 minutes; add water to boil for extraction, and filter; the resulting filtrate is removed by dialysis, adsorbed and decolorized by microfiltration activated carbon, and then heated and concentrated; finally, ethanol (volume concentration: 95% ethanol ...
Embodiment 2
[0124] The preparation method of the lentinan soluble beans for improving intestinal immunity comprises the following steps:
[0125] 1) Lentinan solution is prepared by the following steps:
[0126] a. Preparation of crude lentinan:
[0127] ①Wash well-grown and non-mold mushroom fruiting bodies, cut off the roots, cut the mushroom caps into cubes, dry them at a temperature of 100-120°C, and grind them into powder.
[0128] 2. add cellulase (concentration of cellulase is 0.5wt%, aqueous solution) to powder, enzymolysis is 45min under the condition of 45 ℃ and pH at temperature; then add papain (concentration of papain is 1.5wt%, aqueous solution), enzymolysis at a temperature of 50°C and a pH of 6.1 for 30 minutes; add water to boil for extraction, and filter; the resulting filtrate is dialysis-removed, decolorized by microfiltration activated carbon, and then heated and concentrated; finally, ethanol (volume concentration of 95 % ethanol solution.) Stirring, after static c...
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