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A strain of Bacillus subtilis xdk06 and its application

A technology of Bacillus subtilis and microbial strains is applied in the field of microbial application and fertilizer fermentation, which can solve the problems of pollution sources at the bottom of the pond, fertilizer nutrient loss, and equipment being easily damaged by corrosion, so as to improve the effective utilization rate, the environmental protection of the fermentation process, and the reduction of The effect of labor intensity

Active Publication Date: 2022-08-09
HESHAN SEWETE BIOLOGICAL PROD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the fertilizers fermented by the above-mentioned fermentation methods are all sinking. When applied to the aquaculture water body, they will immediately sink to the bottom of the pond, resulting in greatly reduced fertilizer efficiency, and even form a source of pollution at the bottom of the pond, thus limiting the use of organic fertilizers in aquaculture. application
The fermentation process of the above-mentioned fermentation method will also convert a large amount of carbon and nitrogen nutrients in the organic fertilizer into ammonia and carbon dioxide, thereby volatilizing into the air, causing a foul smell, resulting in a bad production environment, easy corrosion and damage to the equipment, and causing Nutrient loss from fertilizers, wasted energy

Method used

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  • A strain of Bacillus subtilis xdk06 and its application
  • A strain of Bacillus subtilis xdk06 and its application
  • A strain of Bacillus subtilis xdk06 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Strain domestication and screening

[0030] (1) Prepare the acclimation medium

[0031] Firstly, the chicken manure, mushroom residue and rice bran are pretreated such as drying, impurity removal and crushing, and then mixed uniformly according to the weight ratio of chicken manure: mushroom residue: rice bran=30~50:20~30:20~30, for use;

[0032] (2) Prepare the strain to be domesticated

[0033] 12 strains of yeast, 16 strains of Bacillus subtilis and 8 strains of Lactobacillus acidophilus were selected as the strains to be domesticated;

[0034] (3) Environmental conditions for domestication

[0035] An anaerobic fermentation environment with a water content of 50% to 60%, a pH of 4.5 to 7.5, and a temperature of 30 to 50°C;

[0036] (4) Domestication process

[0037] Use a 2-liter conical flask, put 1 kg of the above-mentioned acclimation medium, adjust the water content and pH value according to the above-mentioned conditions, and then respectively ins...

Embodiment 2

[0041]A preparation method of organic nutrients is carried out indoors, and the room temperature is between 20 and 25 DEG C. The preparation steps are as follows: chicken manure, mushroom residue and rice bran are evenly mixed in a weight ratio of 50:25:25, and then piled into piles, The weight of each pile is controlled within 100 tons, and then Bacillus subtilis XDK06 is evenly added to obtain an anaerobic fermentation system. The addition amount of Bacillus subtilis XDK06 is 2% of the weight of the organic material. Anaerobic fermentation was carried out for 60 days, the initial water content of the control material was 50%-60%, the pH value of the anaerobic fermentation system was controlled at 4.5-7.5, the fermentation process was turned once every 20 days, and the whole fermentation process was turned twice in total . During the fermentation process, because the fermented raw materials used (ie chicken manure, mushroom residue and rice bran) have undergone pretreatment s...

Embodiment 3

[0043] A preparation method of organic nutrients is carried out indoors, and the room temperature is higher than 25°C. The preparation steps are as follows: the chicken manure and mushroom residue are evenly mixed at a weight ratio of 70:30, and then piled into piles, and the weight of each pile is controlled at 100 Within 1 ton, then evenly add Bacillus subtilis XDK06 to obtain an anaerobic fermentation system, the addition amount of Bacillus subtilis XDK06 is 5% of the weight of the organic material, and the anaerobic fermentation system is anaerobic fermentation at 30 ℃ ~ 50 ℃ for 20 days, control The initial water content of the material is 50%-60%, the pH value of the anaerobic fermentation system is controlled at 4.5-7.5, and the heap is turned once on the 10th day of fermentation.

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Abstract

The present invention discloses a strain of Bacillus subtilis XDK06, which has been deposited in the Guangdong Provincial Microbial Culture Collection Center on October 30, 2020, and the preservation number is GDMCC No: 61260; the present invention also discloses the strain of the Bacillus subtilis XDK06. Cultivation method and use thereof in organic fertilizer fermentation. The Bacillus subtilis XDK06 has anaerobic fermentation properties, and can ferment organic materials under anaerobic conditions without aeration. It needs to turn the heap 1 or 2 times, which greatly reduces the labor intensity, can fully decompose the organic materials, and retain the nutrients in it to a greater extent. , which can gradually release nutrients and improve the effective utilization of fertilizers.

Description

technical field [0001] The invention belongs to the technical field of microorganism application and fertilizer fermentation, in particular to a Bacillus subtilis XDK06 with anaerobic fermentation properties, and also to the application of the Bacillus subtilis in organic fertilizer fermentation. Background technique [0002] Bacillus subtilis is widely used in the fermentation of organic fertilizers, but the oxygen consumption properties of the existing Bacillus subtilis determine that the fermentation process of organic fertilizers must adopt the method of oxygen consumption fermentation, so the fermentation process must be equipped with aeration facilities to meet the needs of In addition, the fermentation process must be turned over frequently, otherwise the effect of decomposing the material cannot be achieved, and the labor intensity is high. At the same time, in order to achieve the purpose of aerobic fermentation, the requirements for fermentation conditions such as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/20C12R1/125
CPCC12N1/20C05F3/00C05F17/20C05F5/00C05F5/002Y02W30/40
Inventor 杨雄陈永红郑秉能张其辉黄洁容
Owner HESHAN SEWETE BIOLOGICAL PROD
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