Selenium nano composite material capable of overcoming multi-drug-resistant bacterial infection, preparation method and application thereof

A technology of multi-drug-resistant bacteria and composite materials, applied in the field of nanomaterials, can solve the problems of increasing liver and kidney toxicity, penicillin resistance, etc., and achieve the effects of less drug resistance, high safety, and excellent antibacterial activity

Active Publication Date: 2021-03-12
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

Although there have been clinical cases of combining β-lactamase inhibitors with penicillin, even with effective inhibition of β-lactamase-induced drug breakdown, activated drug efflux pumps are still effective and still require higher Penicillin can only play a role in a certain dose, which will not only increase the toxicity of liver and kidney, but also make bacteria more resistant to penicillin, leading to a vicious circle

Method used

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  • Selenium nano composite material capable of overcoming multi-drug-resistant bacterial infection, preparation method and application thereof
  • Selenium nano composite material capable of overcoming multi-drug-resistant bacterial infection, preparation method and application thereof
  • Selenium nano composite material capable of overcoming multi-drug-resistant bacterial infection, preparation method and application thereof

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preparation example Construction

[0025] A kind of preparation method of the selenium nano-composite material that the present invention overcomes multidrug-resistant bacterial infection comprises the following steps:

[0026] Step S1: Dissolve 10mM-50mMD-mannose and 10mM-50mM sodium triacetylborohydride in 10-50mL pure water, place 500mg-1g chitosan in 30mL-50mL volume concentration 1% acetic acid solution, ultrasonic Stir until it is completely dissolved, slowly add chitosan in acetic acid to the mannose solution, stir at room temperature for 48 hours to form a mixed solution, add the mixed solution to a dialysis bag with a molecular weight cut-off of 8-14kDa, and give 150rpm slow stirring at the same time , using ultrapure water dialysis for 72 hours, changing the pure water every 24 hours, and obtaining mannose-modified chitosan by freeze-drying;

[0027] Step S2: Dissolve 80mg-200mg mannose-modified chitosan in 40mL-100mL volume concentration 1% acetic acid solution, and dissolve 10mg-30mg sodium tripolyp...

Embodiment 1

[0030] (1): Dissolve 20mMD-mannose and 20mM sodium triacetylborohydride in 20mL pure water, place 500mg chitosan in 30mL volume concentration 1% acetic acid solution, stir ultrasonically until completely dissolved, and place Slowly add chitosan into the mannose solution, stir at room temperature for 48 hours to form a mixed solution, add the mixed solution to a dialysis bag with a molecular weight cut-off of 8-14kDa, and give slow stirring at 150rpm at the same time, use ultrapure water for dialysis for 72h, every Replace the pure water once every 24 hours, and obtain mannose-modified chitosan by freeze-drying;

[0031] (2): 80mg mannose modified chitosan is dissolved in 40mL volume concentration 1% acetic acid solution, and the sodium tripolyphosphate of 18mg is dissolved in 15mL pure water, then, the 20mM sodium selenite of 1mL, 1mL of 80mM ascorbic acid and 55mg ampicillin were added to the sodium tripolyphosphate solution, and stirred for 10min, then the sodium tripolyphos...

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Abstract

The invention relates to a selenium nano composite material capable of overcoming multi-drug-resistant bacterial infection. The selenium nano composite material comprises nano-selenium composite particles, which are obtained by coating mannose modified chitosan on the outer surface of a complex of selenium nano particles and beta-lactam antibiotics, and are of a spherical structure, wherein selenium nanoparticles and beta-lactam antibiotics are arranged in the nano-selenium composite particles, mannose-modified chitosan is arranged on the outer surface of the nano-selenium composite particles,the average particle size of the nano-selenium composite particles is 40-80 nm, and the thickness of shells of the mannose-modified chitosan on the surface is smaller than or equal to 5 nm. The invention also provides a selenium nano composite material capable of overcoming multi-drug-resistant bacterial infection, and application of the selenium nano composite material in a nano antibacterial material. The nano antibacterial material prepared by the invention inhibits the expression of a drug efflux pump of multi-drug-resistant bacteria and the activity of beta-lactamase, and chitosan is used for embedding selenium nanoparticles and antibiotics, so that the dispersity and stability of the nano material are favorably improved.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a selenium nanocomposite material for overcoming multidrug-resistant bacterial infection, a preparation method and application thereof. Background technique [0002] Bacterial drug resistance caused by clinical abuse of penicillin has seriously affected its clinical efficacy. Designing new drugs or finding new methods to combat penicillin-mediated drug resistance is the biggest clinical challenge. [0003] Activation of drug efflux pumps and overexpression of β-lactamase are the main mechanisms of resistance to penicillin in Escherichia coli. Accumulated studies have confirmed that the bacterial efflux pump system AcrAB-TolC can be activated by reactive oxygen species produced by non-lethal concentrations of antibiotics, on the other hand, β-lactamase can hydrolyze the β-lactam ring of penicillin, destroying the drug structure and making Penicillin loses its an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/04A61K9/51A61K47/36A61K47/54A61K45/06A61K9/19A61P31/04B82Y5/00B82Y30/00B82Y40/00
CPCA61K33/04A61K9/5161A61K45/06A61K9/19A61P31/04B82Y5/00B82Y30/00B82Y40/00A61K47/549
Inventor 孙冬冬汪泽坤王静媛黎雨晴杨恩东郭峰汪维云
Owner ANHUI AGRICULTURAL UNIVERSITY
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