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A kind of sph-ru-mmt@pz nano antibacterial agent and its preparation method and application

A nano antibacterial agent, sph-ru technology, applied in the field of nano materials, can solve problems such as lack of targeting, achieve the effect of improving antibacterial activity and avoiding excessive use

Active Publication Date: 2021-08-24
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Photodynamic antimicrobial therapy is less likely to cause drug resistance, but lacks targeting

Method used

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  • A kind of sph-ru-mmt@pz nano antibacterial agent and its preparation method and application
  • A kind of sph-ru-mmt@pz nano antibacterial agent and its preparation method and application
  • A kind of sph-ru-mmt@pz nano antibacterial agent and its preparation method and application

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preparation example Construction

[0031] The preparation method of a kind of Sph-Ru-MMT@PZ nano antibacterial agent of the present invention comprises the following steps:

[0032] (1) [Ru(bpy) 2 Cl 2 ]0.27g~2.7g and dppz0.15g~1.5g are dissolved in 8.0~20mL ethanol solution, the volume ratio of ethanol and water in the ethanol solution is 3:1, put it into a three-necked flask, add dropwise 500~1500µL polystyrene Suspension, mixed vigorously to obtain a mixture, the mixture was heated to reflux at 100~110°C under argon for 10~12h, the reaction mixture was cooled to room temperature to obtain a yellow solution, and the solvent was removed using a rotary evaporator, and by adding NH 4 PF 6 Precipitate the complex with an aqueous solution, use an 80KHz ultrasonic bath to suspend the solid in ethanol for 3 minutes, wash it centrifugally with tetrahydrofuran to remove polystyrene, and finally wash it centrifugally with deionized water, remove the solvent and dry it to obtain nano-ruthenium with a diameter of 5-25n...

Embodiment 1

[0039] (1) put 0.27g[Ru(bpy) 2 Cl 2 ] and 0.15gdppz dissolved in 8.0mL ethanol solution were put into a three-necked flask, 500µL polystyrene suspension was added dropwise, and the mixture was mixed vigorously to obtain a mixture. The mixture was heated to reflux at 100°C for 10h under argon, and the reaction mixture was cooled to room temperature, a yellow solution was obtained, and the solvent was removed using a rotary evaporator by adding NH 4 PF 6 Aqueous solution to precipitate the complex, use 80KHz ultrasonic bath to suspend the solid in ethanol for 3min, centrifuge wash with tetrahydrofuran to remove polystyrene, finally wash with deionized water, remove solvent and dry to obtain nanometer ruthenium complex particles Sph- Ru.

[0040] (2) Mix 1.0g of montmorillonite MMT with 6.0mL of water and 6.0mL of ethanol in an Erlenmeyer flask, then add 3.0g of cetyltrimethylammonium bromide CTAB to obtain the mixture, stir the reaction, and then use reflux The condenser was...

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Abstract

The present invention relates to a kind of Sph-Ru-MMT@PZ nano antibacterial agent, described Sph-Ru-MMT@PZ nano particle is that montmorillonite MMT and photosensitizer phthalocyanine zinc PZ are sequentially loaded onto nano-ruthenium complex particle Sph-Ru-MMT@PZ The diameter of the nanoparticle Sph-Ru-MMT@PZ obtained on Ru is 85-155nm; the present invention also provides the application of a Sph-Ru-MMT@PZ nano antibacterial agent in the field of anti-Escherichia coli. The MMT of the present invention is loaded on the Sph-Ru nanoparticles, and then anchored on the outer surface of the Escherichia coli membrane, and the membrane-anchored functional photosensitizer zinc phthalocyanine photosensitizer zinc phthalocyanine PZ is irradiated with 670nm near-infrared NIR light, which will By producing reactive oxygen species ROS to destroy the structure of bacteria, resulting in the lysis of Escherichia coli, Sph-Ru-MMT@PZ nano-antibacterial agent has the ability to target the cell membrane and effectively improve the antibacterial activity.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a Sph-Ru-MMT@PZ nanometer antibacterial agent and its preparation method and application. Background technique [0002] Bacterial diseases threaten the health and lives of millions of patients every year, and antibiotics are currently the most common method for treating infectious pathogenic diseases. Traditional antibiotics selectively inhibit or kill microbial cells by preventing cellular processes such as cell replication, repair, protein synthesis and cell wall renewal. Over the years, disease-causing bacteria have developed resistance to almost all types of traditional antibiotics by mutating or acquiring resistance genes from other organisms. However, due to the lack of intuitive results, the therapeutic effect of antibiotics cannot be fed back in time, which can easily lead to drug overdose. [0003] Photodynamic antimicrobial therapy is an effective al...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/22A61K47/02A61K41/00A61K9/51A61P31/04C07F15/00C09K11/06
CPCA61K9/5115A61K41/0071A61P31/04C07F15/0053C09K11/06C09K2211/185Y02A50/30
Inventor 汪维云尹晨阳丁晓远陈小庆杨恩东郭峰孙冬冬
Owner ANHUI AGRICULTURAL UNIVERSITY