A kind of biologically active peptide rrecpsdecgagvf and its preparation method and application

A bioactive peptide and sequence technology, applied in the protein field, can solve the problem of lack of research, achieve the effect of good anti-inflammatory activity, promote the increase of induction and improve the quality of life

Active Publication Date: 2022-05-24
SHANGHAI JIAOTONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the functions of Ubiquitin-40S ribosomal protein S27a protein polypeptide fragments in the prior art

Method used

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  • A kind of biologically active peptide rrecpsdecgagvf and its preparation method and application
  • A kind of biologically active peptide rrecpsdecgagvf and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Artificial synthesis of bioactive peptide RRECPSDECGAGVF

[0033] 1. Synthesis of Bioactive Peptides

[0034] Synthetic bioactive peptide RRECPSDECGAGVF.

[0035] 2. Confirmation of Bioactive Peptides

[0036] 1) UPLC analysis

[0037] The UPLC conditions are as follows:

[0038] Instrument: Waters ACQUITY UPLC ultra-high performance liquid phase, electrospray, quadrupole, time-of-flight mass spectrometer

[0039] Column Specifications: BEH C18 Column

[0040] Flow rate: 0.4mL / min

[0041] Temperature: 50℃

[0042] UV detection wavelength: 210nm

[0043] Injection volume: 2μL

[0044] Gradient conditions: solution A: water containing 0.1% formic acid (v / v), solution B: acetonitrile containing 0.1% formic acid (v / v)

[0045] Time(min) %A %B

0 95.0 5.0

1.50 80.0 20.0

3.50 60.0 40.0

5.00 40.0 60.0

7.00 15.0 85.0

8.00 0.0 100.0

11.00 0.0 100.0

11.50 95.0 5.0

13.00 95.0 5.0

[0046] 2) Mass spectrometry

[0047] The mass spectrometry co...

Embodiment 2

[0061] Example 2 Immunoactivity test of biologically active peptides

[0062] 1. Determination of macrophage nitric oxide induction by bioactive peptide RRECPSDECGAGVF (Griess method)

[0063] 1. Experimental reagents and instruments:

[0064] Reagents: experimental animals balb / c mice (male 6-8 weeks old) spleen lymphocyte-derived bioactive peptide RRECPSDECGAGVF; LPS, purchased from Sigma; neutral red staining solution, produced by Biyuntian Institute of Biotechnology.

[0065] Equipment: LRH-250F biochemical incubator, Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge, Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150 CO 2 Incubator Heraeus; DragonWellscan MK3 Microplate Reader Labsystems.

[0066] 2. Test method:

[0067] The number of cells added is 2 × 10 6 100 μl / well of cell suspension / ml, and 200 μl / well of peptide-containing RPMI1640 complete culture medium (10% FBS) was added after adherence purification. In the inflam...

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Abstract

The present invention relates to the field of proteins, in particular to a biologically active peptide RRECPSDECGAGVF and its preparation method and application. The amino acid sequence of the biologically active peptide RRECPSDECGAGVF is Arg-Arg-Glu-Cys-Pro-Ser-Asp-Glu-Cys-Gly-Ala ‑Gly‑Val‑Phe. After in vitro immunoregulatory function experiments, it was found that the biologically active peptide RRECPSDECGAGVF has a better immune regulatory function. The biologically active peptide RRECPSDECGAGVF of the present invention has the ability to increase the amount of nitric oxide induced by macrophages, can improve the body's ability to resist external pathogen infection, and reduce the incidence of the body; at the same time, it can promote macrophages to secrete cytokines and regulate the body's immunity. Improving the quality of life is of great significance to the development of food, health products and medicines with immune regulation function.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active peptide RRECPSDECGAGVF and a preparation method and application thereof. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anticancer, blood pressure lowering, antibacterial, cholesterol lowering, antidiabetic and other properties. More than 3000 different bioactive peptides have been reported in BIOPEP-UMW, the most authoritative bioactive peptide database. [0003] At present, the research on bioactive peptides mostly focuses on food-derived peptides, and there are few studies and reports on non-food-derived peptides. And research results have confirmed that, compared wit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12A61K8/64A61K38/17A61Q19/00A61P29/00A61P37/02A23L33/18
CPCC07K14/47A61K8/64A61Q19/00A61P29/00A61P37/02A23L33/18A61K38/00A23V2002/00A23V2200/324A23V2250/55
Inventor 张少辉栾媛媛王娟张伯宇占文静李政郭婷婷谢雨佳
Owner SHANGHAI JIAOTONG UNIV
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