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Method for simultaneously detecting or identifying effective components in heart-soothing lipid-lowering tablet

An active ingredient and lipid-lowering technology, which is applied in the field of detection or identification of active ingredients in Shuxin Jiangzhi Tablets and simultaneous detection or identification of active ingredients in Shuxin Jiangzhi Tablets. TLC, mutual interference and other problems of polydatin and polydatin, to achieve the effect of saving detection time and cost, strong specificity, and improved specificity

Active Publication Date: 2021-03-19
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Since the molecular structures of puerarin and polydatin are relatively close, their physical properties and solubility are relatively similar, resulting in conventional thin-layer development conditions, such as chloroform-methanol-water (7:2.5:0.25), which can be detected respectively One substance, but if the above two substances are contained in a preparation prescription, there will be mutual interference, resulting in non-specificity of the detection method
Among the currently published detection methods, there is no TLC method for the simultaneous detection of puerarin and polydatin

Method used

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  • Method for simultaneously detecting or identifying effective components in heart-soothing lipid-lowering tablet
  • Method for simultaneously detecting or identifying effective components in heart-soothing lipid-lowering tablet
  • Method for simultaneously detecting or identifying effective components in heart-soothing lipid-lowering tablet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1HPLC measures the content of puerarin, polydatin, salvianolic acid B

[0046] The present embodiment simultaneously detects the method for active ingredient in Shuxin Jiangzhi Tablets, as follows:

[0047]1. Preparation of reference substance solution: Take appropriate amount of puerarin, polydatin and salvianolic acid B reference substance, weigh them accurately, add dilute ethanol to make puerarin containing 190 μg per 1 ml, polydatin per 1 ml containing 75 μg, salvianolic acid Each 1ml of B contains 240μg mixed control solution.

[0048] 2. Preparation of the test solution: Accurately weigh 0.3g of Shuxin Jiangzhi Tablets, put it in a 10ml measuring bottle, add dilute ethanol to the mark, shake well, ultrasonicate, power 250W, frequency 50kHz, process 20min, use 0.45μm micrometer Pore ​​membrane filtration, that is.

[0049] 3. Preparation of negative control solution: Accurately weigh 0.3g of the negative sample lacking puerarin, polydatin, and salvian...

Embodiment 2

[0079] The content of puerarin, polydatin, salvianolic acid B in the embodiment 2 HPLC determination Shuxin Jiangzhi Tablets

[0080] The present embodiment simultaneously detects the method for active ingredient in Shuxin Jiangzhi Tablets, as follows:

[0081] 1. The preparation of reference substance solution, the preparation of negative control solution: with embodiment 1.

[0082] 2. Preparation of the test solution: take the test samples SXJZ-20190301, SXJZ-20190302, SXJZ-20190303 and the marketed products 20161102, 20170404, 20170701, the preparation method is the same

[0083] Example 1.

[0084] 3. Chromatographic conditions and system suitability test: the same as in Example 1.

[0085] 4. Determination method: respectively draw and mix 10 μL of the reference substance solution and the test solution, inject it into the liquid chromatograph, measure it, and obtain it.

[0086] 5. Results: All components met the baseline analysis requirements. The theoretical plate n...

Embodiment 3

[0089] Example 3 Identification of the same puerarin and polydatin TLC

[0090] In this embodiment, 3 batches of products (batch numbers: 20161102, 20170404, 20170701) and 1 batch of laboratory samples are identified, as follows:

[0091] Take Shuxin Jiangzhi Tablets, grind finely, accurately weigh 5g, add 20ml of methanol, ultrasonicate for 20 minutes, filter, and concentrate the filtrate to 10ml as the test solution.

[0092] Take 3g of the negative sample lacking kudzu root and Polygonum cuspidatum, grind it finely, add 20ml of methanol, ultrasonicate for 20 minutes, filter, concentrate the filtrate to 10ml, and use it as the negative sample solution.

[0093] Another puerarin, polydatin reference substance, were added methanol to make each 1ml solution containing 1mg, as the reference solution.

[0094] According to the thin-layer chromatography test, absorb 5 μl of each of the above four solutions, and spot them on the same high-efficiency silica gel G thin-layer plate t...

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Abstract

The invention provides a method for simultaneously detecting or identifying effective components in a heart-soothing lipid-lowering tablet. The method comprises the following steps of preparing a testsolution, preparing a reference solution and determining by high performance liquid chromatography. According to the method, the detection time and detection cost are saved, meanwhile, the correlation of the three components in the heart-soothing lipid-lowering tablet is analyzed, and the quality of the heart-soothing lipid-lowering tablet is comprehensively evaluated and controlled, so a comprehensive and reliable basis is provided for true and false identification and internal quality detection of the heart-soothing lipid-lowering tablet, and accuracy of the heart-soothing lipid-lowering tablet is improved; stability of product quality and safety and effectiveness of clinical medication are ensured. The method has advantages of good stability, good repeatability, high recovery rate andstrong specificity.

Description

technical field [0001] The invention relates to a method for detecting or identifying active ingredients in Shuxin Jiangzhi Tablets, in particular to a method for simultaneously detecting or identifying active ingredients in Shuxin Jiangzhi Tablets, belonging to the field of drug testing. Background technique [0002] Shuxin Jiangzhi Tablets is a drug approved by the State Food and Drug Administration. It is composed of 11 herbs such as purple salvia, hawthorn, knotweed, kudzu root, and red peony root. In the prescription, purple salvia miltiorrhiza is reused, which can cool blood and eliminate addiction, and can also promote blood circulation and relieve pain. A film-coated tablet prepared by extracting its active ingredients by a modern extraction method, the Chinese patent CN103690750B discloses its preparation method. [0003] The current standard of Shuxin Jiangzhi Tablets is recorded in the Food and Drug Administration WS3-B-2799-2002 standard, which does not establis...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06G01N30/90
CPCG01N30/88G01N30/06G01N30/90G01N2030/884G01N2030/047Y02A50/30
Inventor 李俊苏莎饶高雄邓雪琪罗娇
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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